PMID- 26628956
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151202
LR  - 20200929
IS  - 1940-5901 (Print)
IS  - 1940-5901 (Electronic)
IS  - 1940-5901 (Linking)
VI  - 8
IP  - 9
DP  - 2015
TI  - Dexmedetomidine alleviates the spinal cord ischemia-reperfusion injury through 
      blocking mast cell degranulation.
PG  - 14741-9
AB  - OBJECTIVE: To investigate the neuro-protective effects of dexmedetomidine (dex) 
      on I/R-induced spinal injury and potential mechanisms. METHODS: sprague-Dawley 
      rats in the treatment group received intraperitoneal injections of 25 mg/kg 
      dexmedetomidine, MC stabilizer cromolyn (100 mg/kg), MCs stimuliser compound 
      48/80 (80 mg/kg), PBS at 24 h befor IR. Underwent 5 minutes of aortic occlusion 
      via median sternotomy, functional scores were recorded at 12, 24, 36 and 48 hours 
      after reperfusion. Additionally, 3 mice underwent sham surgery with sternotomy 
      and dissection of the aorta and subclavian artery with no occlusion. Spinal cords 
      were examined for protein kinase B (AKT), CREB, and brain-derived neurotrophic 
      factor (BDNF) following treatment alone or ischemia-reperfusion surgery. 
      Collected the serum to observe the expression of pro-inflammation cytokines 
      (TNF-alpha, INF-gamma and IL-1beta) and anti-inflammation cytokines (TGF-beta, IL-10 and IL-6). 
      Then the MCs were harvested to test the expression surface molecular of FcepsilonR and 
      MCs' degranulation. RESULTS: Pretreated the rats with dexmedetomidine has higher 
      neurologic function at all time points after I/R injury. We collected the serum 
      of rats then detected the pro-inflammation cytokines TNF-alpha, INF-gamma and IL-1beta 
      levels and anti-inflammation cytokinses TGF-beta, IL-10 and IL-6 levels, found that 
      the pro-inflammation cytokines of dexmedetomidine group was decreased whereas the 
      anti-inflammation cytokinses was increased. At the same time the protect protein 
      of AKT, CREB and mRNA BDNF were increased. They had the same results with 
      cromolyn group, and opposite with the compound 48/80 group. We pretreated MCs 
      with dexmedetomidine in vitro, and found that the activity surface molecular of 
      MCs was down-regulation, and MCs degranulation was decreased. CONCLUSION: We thus 
      demonstrate a possible mechanism by which dexmedetomidine alleviates spinal cord 
      I/R injury through blocking the MCs degranulation.
FAU - Ma, Jun
AU  - Ma J
AD  - Department of Neurosurgery, Shaoxing Central Hospital Shaoxing, 312003, China.
FAU - Zhang, Xiao-Long
AU  - Zhang XL
AD  - Department of Intensive Care Unit, The Second Affiliated Hospital & Yuying 
      Children's Hospital of Wenzhou Medical University Wenzhou, 325027, China.
FAU - Wang, Cheng-Yu
AU  - Wang CY
AD  - Department of Anesthesiology, The Second Affiliated Hospital & Yuying Children's 
      Hospital of Wenzhou Medical University Wenzhou, 325027, China.
FAU - Lin, Zhi
AU  - Lin Z
AD  - Department of Anesthesiology, The Second Affiliated Hospital & Yuying Children's 
      Hospital of Wenzhou Medical University Wenzhou, 325027, China.
FAU - Tao, Jie-Ru
AU  - Tao JR
AD  - Department of Anesthesiology, The Second Affiliated Hospital & Yuying Children's 
      Hospital of Wenzhou Medical University Wenzhou, 325027, China.
FAU - Liu, Hua-Cheng
AU  - Liu HC
AD  - Department of Anesthesiology, The Second Affiliated Hospital & Yuying Children's 
      Hospital of Wenzhou Medical University Wenzhou, 325027, China.
LA  - eng
PT  - Journal Article
DEP - 20150915
PL  - United States
TA  - Int J Clin Exp Med
JT  - International journal of clinical and experimental medicine
JID - 101471010
PMC - PMC4658845
OTO - NOTNLM
OT  - Dexmedetomidine
OT  - degranulation
OT  - ischemia-reperfusion injury
OT  - mast cell
EDAT- 2015/12/03 06:00
MHDA- 2015/12/03 06:01
PMCR- 2015/09/15
CRDT- 2015/12/03 06:00
PHST- 2015/02/15 00:00 [received]
PHST- 2015/05/28 00:00 [accepted]
PHST- 2015/12/03 06:00 [entrez]
PHST- 2015/12/03 06:00 [pubmed]
PHST- 2015/12/03 06:01 [medline]
PHST- 2015/09/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Med. 2015 Sep 15;8(9):14741-9. eCollection 2015.