PMID- 26629026
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151202
LR  - 20200929
IS  - 1940-5901 (Print)
IS  - 1940-5901 (Electronic)
IS  - 1940-5901 (Linking)
VI  - 8
IP  - 9
DP  - 2015
TI  - Function of CD163 fragments in porcine reproductive and respiratory syndrome 
      virus infection.
PG  - 15373-82
AB  - Monocyte/macrophage scavenger receptor CD163 plays an important role in porcine 
      reproductive and respiratory syndrome virus (PRRSV) infection. To identify the 
      domains of CD163 involved in PRRSV infection, CD163 fragments P1 (1-798 bp), P2 
      (790-2046 bp), P3 (2023-3345 bp), Y-P1 (160-798 bp), Y-P2 (790-2046 bp) and Y-P3 
      (2143-3084 bp) were expressed by eukaryotic and prokaryotic expression systems, 
      respectively. Infection experiments revealed that non-permissive BHK-21 cells 
      transfected with pCD163 could be infected by PRRSV. However, cells with truncated 
      CD163 (P1, P2, or P3) were not susceptible to PRRSV. Meanwhile, Y-P1, Y-P2, and 
      Y-P3 were expressed in E. coli and antisera to these peptides were prepared in 
      mice. A virus blocking test showed that Y-P2 protein and anti-Y-P2 mouse serum 
      could block PRRSV infection in a dose-dependent manner, while Y-P3 protein could 
      improve virus infection.
FAU - Li, Hong
AU  - Li H
AD  - Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of 
      Agricultural Sciences Shanghai 201106, China.
FAU - Zhou, En-Min
AU  - Zhou EM
AD  - Veterinary Immunology Institute of Northwest A & F University, College of 
      Veterinary Medicine, Northwest A & F University Yangling 712100, Shaanxi 
      Province, China.
FAU - Liu, Cheng-Qian
AU  - Liu CQ
AD  - Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of 
      Agricultural Sciences Shanghai 201106, China.
FAU - Yi, Jian-Zhong
AU  - Yi JZ
AD  - Institute of Animal Husbandry and Veterinary Science, Shanghai Academy of 
      Agricultural Sciences Shanghai 201106, China.
LA  - eng
PT  - Journal Article
DEP - 20150915
PL  - United States
TA  - Int J Clin Exp Med
JT  - International journal of clinical and experimental medicine
JID - 101471010
PMC - PMC4658915
OTO - NOTNLM
OT  - CD163 fragments
OT  - PRRSV
OT  - infection
OT  - virus blocking
EDAT- 2015/12/03 06:00
MHDA- 2015/12/03 06:01
PMCR- 2015/09/15
CRDT- 2015/12/03 06:00
PHST- 2015/05/10 00:00 [received]
PHST- 2015/06/26 00:00 [accepted]
PHST- 2015/12/03 06:00 [entrez]
PHST- 2015/12/03 06:00 [pubmed]
PHST- 2015/12/03 06:01 [medline]
PHST- 2015/09/15 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Med. 2015 Sep 15;8(9):15373-82. eCollection 2015.