PMID- 26632186
OWN - NLM
STAT- MEDLINE
DCOM- 20160920
LR  - 20181202
IS  - 1347-5215 (Electronic)
IS  - 0918-6158 (Linking)
VI  - 38
IP  - 12
DP  - 2015
TI  - Compound 48/80, a Mast Cell Stimulator, Enhances Synthesis of IgE and IgG Induced 
      by Intranasal Application of Ovalbumin in Mice.
PG  - 1954-9
LID - 10.1248/bpb.b15-00637 [doi]
AB  - Mast cells are well established effector cells of type I hypersensitivity 
      reactions such as allergic rhinitis. However, recent studies have suggested that 
      activated mast cells enhance local immunoglobulin E (IgE) synthesis in the nasal 
      mucosa of allergic rhinitis patients. Therefore, we hypothesized that 
      non-immunological mast cell activators may have the potential to enhance local 
      IgE synthesis. Here, we examined the effect of compound 48/80 (C48/80), a mast 
      cell activator, on IgE and immunoglobulin G (IgG) synthesis. Female Balb/c mice 
      were intranasally administered a mixture of ovalbumin (OVA) (1-10 microg/nose) and 
      C48/80 (1-100 microg/nose) on days 0, 7, 14 and 21 and on consecutive days from day 
      28 to day 42. Intranasal administration of C48/80 with OVA increased serum 
      OVA-specific IgE and IgG. Double staining with fluorescent-labeled OVA and 
      fluorescent-labeled IgE- or IgG-specific antibody demonstrated the presence of 
      OVA-specific IgE- or IgG-producing cells in the nasal mucosa of sensitized mice. 
      Moreover, intranasal administration of C48/80 with OVA increased the nasal 
      mucosal interleukin (IL)-4 level and enhanced the OVA-induced symptom of 
      sneezing. These results suggested that simultaneous activation of mast cells with 
      antigen exposure enhances local IgE and IgG synthesis.
FAU - Matsui, Nobuaki
AU  - Matsui N
AD  - Department of Pharmacology, Faculty of Pharmaceutical Sciences, Tokushima Bunri 
      University.
FAU - Ito, Daisuke
AU  - Ito D
FAU - Takabatake, Yukari
AU  - Takabatake Y
FAU - Nashioka, Eri
AU  - Nashioka E
FAU - Tada, Shingo
AU  - Tada S
FAU - Kanagawa, Masato
AU  - Kanagawa M
FAU - Fukuishi, Nobuyuki
AU  - Fukuishi N
FAU - Akagi, Masaaki
AU  - Akagi M
LA  - eng
PT  - Journal Article
PL  - Japan
TA  - Biol Pharm Bull
JT  - Biological & pharmaceutical bulletin
JID - 9311984
RN  - 0 (Immunoglobulin G)
RN  - 207137-56-2 (Interleukin-4)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 4091-50-3 (p-Methoxy-N-methylphenethylamine)
RN  - 9006-59-1 (Ovalbumin)
SB  - IM
MH  - Administration, Intranasal
MH  - Animals
MH  - Female
MH  - Immunoglobulin E/*metabolism
MH  - Immunoglobulin G/*metabolism
MH  - Interleukin-4/metabolism
MH  - Mast Cells/*drug effects/metabolism
MH  - Mice, Inbred BALB C
MH  - Mucous Membrane/metabolism
MH  - Nasal Mucosa/*metabolism
MH  - Ovalbumin/*immunology
MH  - Rhinitis, Allergic/*immunology/metabolism
MH  - p-Methoxy-N-methylphenethylamine/*pharmacology
EDAT- 2015/12/04 06:00
MHDA- 2016/09/22 06:00
CRDT- 2015/12/04 06:00
PHST- 2015/12/04 06:00 [entrez]
PHST- 2015/12/04 06:00 [pubmed]
PHST- 2016/09/22 06:00 [medline]
AID - 10.1248/bpb.b15-00637 [doi]
PST - ppublish
SO  - Biol Pharm Bull. 2015;38(12):1954-9. doi: 10.1248/bpb.b15-00637.