PMID- 26632463
OWN - NLM
STAT- MEDLINE
DCOM- 20160510
LR  - 20200717
IS  - 0253-2727 (Print)
IS  - 2707-9740 (Electronic)
IS  - 0253-2727 (Linking)
VI  - 36
IP  - 11
DP  - 2015 Nov
TI  - [Application of BIOMED- 2 standardized Ig gene rearrangement system in multiple 
      myeloma].
PG  - 918-21
LID - 10.3760/cma.j.issn.0253-2727.2015.11.006 [doi]
AB  - OBJECTIVE: To explore the application of BIOMED- 2 standardized immunoglobulin 
      (Ig) gene rearrangement system in the diagnosis of multiple myeloma (MM), and the 
      significance of clonality analysis by multiplex-PCR amplifications. METHODS: A 
      total of 167 cases of MM bone marrow samples from 2009 to 2013, and 20 cases of 
      reactive plasmacytosis used as the controls were included in this study. 
      Multiplex-PCR amplifications were performed and the Ig gene rearrangements were 
      analyzed using BIOMED-2 standardized clonality analysis system. RESULTS: 1 in circle Of 167 
      MM cases, 107 showed IgH VH-JH rearrangement, 33 showed IgH DH-JH rearrangement, 
      and 30% showed IgH DH-JH rearrangement in 60 IgH VH-JH rearrangement negative MM 
      cases. The difference was statistically significant between IgH VH-JH 
      rearrangement positive and negative cases (14.0% vs 30.0%, P=0.032). The total 
      positive rate of IgH VH-JH, IgH DH-JH and IgK was 94.6%. The 20 reactive 
      plasmacytosis (RP) cases showed negative Ig gene rearrangement. 2 of 167 MM 
      cases, 9 (5.4%) showed clonal IgH rearrangement by agarose electrophoresis were 
      confirmed as polyclonality by capillary electrophoresis. 3 in circle Of 53 MM cases who 
      have been detected by Ig gene rearrangement system and fluorescence in situ 
      hybridization (FISH) for IgH simultaneously, 36 showed IgH rearrangement, 26 
      showed FISH IgH positive, and the difference was statistically significant (67.9% 
      vs 49.1%, P=0.049). CONCLUSION: Combined detection of IgH VH- JH, IgH DH- JH and 
      IgK could improve the positive rate of MM clonality dramatically, and measurement 
      of IgH DH-JH rearrangement was more important in the IgH VH- JH negative cases. 
      Ig gene rearrangement system was a faster and more sensitive method than FISH 
      IgH. Application of BIOMED- 2 standardized immunoglobulin (Ig) gene rearrangement 
      system is of significance for MM diagnosis.
FAU - Ai, Xiaofei
AU  - Ai X
AD  - Department of Pathology, Institute of Hematology and Blood Diseases Hospital, 
      CAMS & PUMC, Tianjin 300020, China.
FAU - Wang, Xiaojing
AU  - Wang X
AD  - Department of Pathology, Institute of Hematology and Blood Diseases Hospital, 
      CAMS & PUMC, Tianjin 300020, China.
FAU - Wang, Bo
AU  - Wang B
FAU - Chen, Shenghua
AU  - Chen S
AD  - Department of Pathology, Institute of Hematology and Blood Diseases Hospital, 
      CAMS & PUMC, Tianjin 300020, China.
FAU - Wang, Jun
AU  - Wang J
AD  - Department of Pathology, Institute of Hematology and Blood Diseases Hospital, 
      CAMS & PUMC, Tianjin 300020, China.
FAU - Li, Qinghua
AU  - Li Q
AD  - Department of Pathology, Institute of Hematology and Blood Diseases Hospital, 
      CAMS & PUMC, Tianjin 300020, China.
FAU - An, Gang
AU  - An G
AD  - Department of Pathology, Institute of Hematology and Blood Diseases Hospital, 
      CAMS & PUMC, Tianjin 300020, China.
FAU - Ru, Kun
AU  - Ru K
AD  - Department of Pathology, Institute of Hematology and Blood Diseases Hospital, 
      CAMS & PUMC, Tianjin 300020, China.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
RN  - 0 (Immunoglobulins)
SB  - IM
MH  - Humans
MH  - Immunoglobulins/*genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Multiple Myeloma/*diagnosis/*genetics
MH  - Polymerase Chain Reaction
MH  - *V(D)J Recombination
PMC - PMC7342417
EDAT- 2015/12/04 06:00
MHDA- 2016/05/11 06:00
PMCR- 2015/11/01
CRDT- 2015/12/04 06:00
PHST- 2015/12/04 06:00 [entrez]
PHST- 2015/12/04 06:00 [pubmed]
PHST- 2016/05/11 06:00 [medline]
PHST- 2015/11/01 00:00 [pmc-release]
AID - cjh-36-11-918 [pii]
AID - 10.3760/cma.j.issn.0253-2727.2015.11.006 [doi]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2015 Nov;36(11):918-21. doi: 
      10.3760/cma.j.issn.0253-2727.2015.11.006.