PMID- 26633319 OWN - NLM STAT- MEDLINE DCOM- 20160915 LR - 20220410 IS - 1420-3049 (Electronic) IS - 1420-3049 (Linking) VI - 20 IP - 12 DP - 2015 Nov 27 TI - Improved Antioxidant Capacity of Optimization of a Self-Microemulsifying Drug Delivery System for Resveratrol. PG - 21167-77 LID - 10.3390/molecules201219750 [doi] AB - The use of nano-encapsulated resveratrol (RSV) in self-micro-emulsified drug delivery systems (SMEDDS) formulations was investigated. Self-emulsifying grading tests were used to establish the optimal ratio of oil, surfactant, and co-surfactant. The optimized system was further investigated for the droplet size and zeta potential at the different medium pH values by a Malvern Zetasizer and transmission electron microscopy (TEM). The antioxidant capacity and cytotoxicity of the formulation were detected by DCFH-DA and a CCK-8 assays. The results showed that the nano-emulsion based on ethyl oleate, Tween-80, and PEG-400 (35:40:25, w/w/w) was the most stable formulation due to the small droplet size (approximately 50 nm) and high zeta potential in a neutral environment. Furthermore, this formulation also exhibited a greater antioxidant capacity with less toxicity than free RSV. Taken together, considering these results and the simple fabrication process, this formulation could be used to deliver nutritional food supplements in a stable, efficient, and safe manner. FAU - Chen, Ying AU - Chen Y AD - College of Life Sciences, Henan Normal University, Xinxiang 453007, China. yingch0816@163.com. FAU - Zhang, Huiyong AU - Zhang H AD - College of Life Science and Biotechnology, Xinxiang Medical University, Xinxiang 453003, China. huiyongzhang115@163.com. FAU - Yang, Jing AU - Yang J AD - School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China. yangjing_228@sohu.com. FAU - Sun, Haiyan AU - Sun H AD - School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003, China. haiyan20050101@163.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20151127 PL - Switzerland TA - Molecules JT - Molecules (Basel, Switzerland) JID - 100964009 RN - 0 (Antioxidants) RN - 0 (Drug Carriers) RN - 0 (Emulsions) RN - 0 (Reactive Oxygen Species) RN - 0 (Stilbenes) RN - Q369O8926L (Resveratrol) SB - IM MH - Animals MH - Antioxidants/chemistry/*pharmacology MH - Biological Availability MH - Cell Proliferation/drug effects MH - *Drug Carriers MH - *Drug Delivery Systems MH - *Emulsions MH - PC12 Cells MH - Rats MH - Reactive Oxygen Species/*metabolism MH - Resveratrol MH - Stilbenes/chemistry/*pharmacology PMC - PMC6331851 OTO - NOTNLM OT - antioxidant OT - cytotoxicity OT - nano-encapsulation OT - oil-in-water OT - resveratrol OT - self-emulsifying COIS- The authors declare no conflict of interest. EDAT- 2015/12/04 06:00 MHDA- 2016/09/16 06:00 PMCR- 2015/11/27 CRDT- 2015/12/04 06:00 PHST- 2015/09/16 00:00 [received] PHST- 2015/11/13 00:00 [revised] PHST- 2015/11/17 00:00 [accepted] PHST- 2015/12/04 06:00 [entrez] PHST- 2015/12/04 06:00 [pubmed] PHST- 2016/09/16 06:00 [medline] PHST- 2015/11/27 00:00 [pmc-release] AID - molecules201219750 [pii] AID - molecules-20-19750 [pii] AID - 10.3390/molecules201219750 [doi] PST - epublish SO - Molecules. 2015 Nov 27;20(12):21167-77. doi: 10.3390/molecules201219750.