PMID- 26633527
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151204
LR  - 20200929
IS  - 2076-393X (Print)
IS  - 2076-393X (Electronic)
IS  - 2076-393X (Linking)
VI  - 3
IP  - 4
DP  - 2015 Nov 27
TI  - Immune Response of Multiparous Hyper-Immunized Sows against Peptides from 
      Non-Structural and Structural Proteins of PRRSV.
PG  - 973-87
LID - 10.3390/vaccines3040973 [doi]
AB  - The purpose of this study was to evaluate the humoral and cellular responses of 
      commercial multiparous and hyper-immunized sows against peptides from 
      non-structural (nsp) and structural proteins of porcine reproductive and 
      respiratory syndrome virus (PRRSV). We selected sows with different numbers of 
      parities from a commercial farm. Management practices on this farm include the 
      use of the MLV commercial vaccine four times per year, plus two vaccinations 
      during the acclimation period. The humoral response was evaluated via the 
      antibody recognition of peptides from nsp and structural proteins, and the 
      cellular response was assessed by measuring the frequency of peptide and 
      PRRSV-specific IFN-gamma-secreting cells (IFNgamma-SC). Our results show that sows 
      with six parities have more antibodies against peptides from structural proteins 
      than against peptides from nsp. The analysis of the cellular response revealed 
      that the number of immunizations did not affect the frequency of IFNgamma-SC and that 
      the response was stronger against peptides from structural proteins (M protein) 
      than against nsp (nsp2). In summary, these results demonstrate that multiparous, 
      hyper-immunized sows have a stronger immune humoral response to PRRSV structural 
      peptides than nsp, but no differences in IFNgamma-SC against the same peptides were 
      observed.
FAU - Rascon-Castelo, Edgar
AU  - Rascon-Castelo E
AD  - Laboratorio de Inmunologia, Centro de Investigacion en Alimentacion y Desarrollo 
      A.C (CIAD) Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83304, Mexico. 
      erascon83@gmail.com.
FAU - Burgara-Estrella, Alexel
AU  - Burgara-Estrella A
AD  - Laboratorio de Inmunologia, Centro de Investigacion en Alimentacion y Desarrollo 
      A.C (CIAD) Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83304, Mexico. 
      alexel.burgara@gmail.com.
FAU - Resendiz-Sandoval, Monica
AU  - Resendiz-Sandoval M
AD  - Laboratorio de Inmunologia, Centro de Investigacion en Alimentacion y Desarrollo 
      A.C (CIAD) Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83304, Mexico. 
      mresendiz@ciad.mx.
FAU - Hernandez-Lugo, Andres
AU  - Hernandez-Lugo A
AD  - Consultoria Agropecuaria del Noroeste, Hermosillo, Sonora 83106, Mexico. 
      ahernandez_lugo@hotmail.com.
FAU - Hernandez, Jesus
AU  - Hernandez J
AD  - Laboratorio de Inmunologia, Centro de Investigacion en Alimentacion y Desarrollo 
      A.C (CIAD) Carretera a la Victoria Km 0.6, Hermosillo, Sonora 83304, Mexico. 
      jhdez@ciad.mx.
LA  - eng
PT  - Journal Article
DEP - 20151127
PL  - Switzerland
TA  - Vaccines (Basel)
JT  - Vaccines
JID - 101629355
PMC - PMC4693227
OTO - NOTNLM
OT  - IFN-gamma secreting cells
OT  - PRRSV
OT  - non-structural proteins
OT  - peptides
OT  - structural proteins
EDAT- 2015/12/04 06:00
MHDA- 2015/12/04 06:01
PMCR- 2015/11/27
CRDT- 2015/12/04 06:00
PHST- 2015/09/23 00:00 [received]
PHST- 2015/11/12 00:00 [revised]
PHST- 2015/11/23 00:00 [accepted]
PHST- 2015/12/04 06:00 [entrez]
PHST- 2015/12/04 06:00 [pubmed]
PHST- 2015/12/04 06:01 [medline]
PHST- 2015/11/27 00:00 [pmc-release]
AID - vaccines3040973 [pii]
AID - vaccines-03-00973 [pii]
AID - 10.3390/vaccines3040973 [doi]
PST - epublish
SO  - Vaccines (Basel). 2015 Nov 27;3(4):973-87. doi: 10.3390/vaccines3040973.