PMID- 26634533
OWN - NLM
STAT- MEDLINE
DCOM- 20160915
LR  - 20151204
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 14
IP  - 4
DP  - 2015 Dec 2
TI  - Matrix metalloproteinase-3 gene polymorphism and its mRNA expression in 
      rheumatoid arthritis.
PG  - 15652-9
LID - 10.4238/2015.December.1.17 [doi]
AB  - Matrix metalloproteinase-3 (MMP-3) can mediate the occurrence and development of 
      rheumatoid arthritis (RA). The MMP3 promoter gene exhibits polymorphism with 
      5A/6A alleles. We investigated the correlation between the expression of MMP3 
      gene polymorphism and RA to provide an objective basis for prognosis evaluation. 
      We enrolled 80 RA patients and 80 healthy subjects. Enzyme-linked immunosorbent 
      assay was used to detect MMP-3 serum levels, pyrosequencing was used to test MMP3 
      genotypes, and real-time polymerase chain reaction determined MMP-3 mRNA 
      expression levels. Compared with the control group, the serum level of MMP-3 in 
      the RA patients increased significantly (P < 0.05). The serum level of MMP-3 in 
      RA patients in the active period was markedly elevated compared with that in 
      patients in the relief period (P < 0.05). There was no statistically significant 
      difference between MMP3 gene frequency distribution in the RA patients and the 
      control group (P > 0.05). MMP-3 mRNA expression in the RA patients was markedly 
      upregulated compared with the control group (P < 0.05), while RA patients in the 
      active period exhibited higher MMP-3 mRNA expression (P < 0.05). There was no 
      significant difference in MMP-3 mRNA expression between RA patients with or 
      without the 6A/6A genotype (P > 0.05). RA patients exhibited higher serum MMP-3 
      levels and mRNA expression, which were more obvious in the active period. MMP-3 
      is associated with the occurrence and development of RA bone erosion, and its 
      serum level and mRNA expression can be treated as important predictors of joint 
      damage.
FAU - Ma, M J
AU  - Ma MJ
AD  - Department of Surgery, Victory Hospital of China Petrochemical Group, Victory 
      Petroleum Administration, Dongying, Shandong, China.
FAU - Liu, H C
AU  - Liu HC
AD  - Department of Orthopedics, Affiliated Hospital of Weifang Medical University, 
      Weifang, Shandong, China.
FAU - Qu, X Q
AU  - Qu XQ
AD  - Orthopaedic Institute of PLA, 89th Hospital, Weifang, Shandong, China.
FAU - Wang, J L
AU  - Wang JL
AD  - Orthopaedic Institute of PLA, 89th Hospital, Weifang, Shandong, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151202
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (RNA, Messenger)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Alleles
MH  - Arthritis, Rheumatoid/blood/diagnosis/*genetics
MH  - Case-Control Studies
MH  - Female
MH  - *Gene Expression
MH  - Gene Frequency
MH  - Genotype
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinase 3/blood/*genetics
MH  - Middle Aged
MH  - *Polymorphism, Single Nucleotide
MH  - Promoter Regions, Genetic
MH  - RNA, Messenger/*genetics
MH  - Young Adult
EDAT- 2015/12/05 06:00
MHDA- 2016/09/16 06:00
CRDT- 2015/12/05 06:00
PHST- 2015/12/05 06:00 [entrez]
PHST- 2015/12/05 06:00 [pubmed]
PHST- 2016/09/16 06:00 [medline]
AID - gmr6304 [pii]
AID - 10.4238/2015.December.1.17 [doi]
PST - epublish
SO  - Genet Mol Res. 2015 Dec 2;14(4):15652-9. doi: 10.4238/2015.December.1.17.