PMID- 26635145
OWN - NLM
STAT- MEDLINE
DCOM- 20161006
LR  - 20181113
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 5
DP  - 2015 Dec 4
TI  - Busulfan and cyclosphamide induce liver inflammation through NLRP3 activation in 
      mice after hematopoietic stem cell transplantation.
PG  - 17828
LID - 10.1038/srep17828 [doi]
LID - 17828
AB  - The aim of this study was to evaluate the role of NLRP3 inflammasome on 
      BU/CY-induced liver inflammation in mice after HSCT. HSCT mice model was 
      established through infusion of 5 x 10(6) bone marrow mononuclear cells after 
      conditioned with BU/CY. On day 7, 14, 21 and 28 after HSCT, mice were sacrificed 
      for analysis of liver inflammation, cytokine secretion, NLRP3 expression and 
      caspase-1 activation as well as release of ATP and high-mobility group protein B1 
      (HMGB1). Furthermore, NLRP3 selective inhibitor (BAY 11-7082) was administrated 
      into mice after HSCT to evaluate its effects on liver inflammation. Severe liver 
      inflammation and damage with elevated secretion of IL-1beta and IL-18 were found in 
      mice after HSCT. Meanwhile, elevated expressions of NLRP3 and caspase-1 
      activation in liver were found. In addition, increased release of ATP and HMGB1 
      were observed. Selective inhibition of NLRP3 decreased caspase-1 activation and 
      secretion of IL-1beta and IL-18. Furthermore, NLRP3 inhibition also reduced 
      infiltration of macrophages and neutrophils and improved liver function. In 
      conclusion, NLRP3 was involved in BU/CY-induced liver inflammation after HSCT and 
      selectively inhibited it ameliorated liver inflammation and improved liver 
      function, suggesting targeting NLRP3 might be a new approach in the prophylaxis 
      of liver inflammation after HSCT.
FAU - Qiao, Jianlin
AU  - Qiao J
AD  - Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, 
      Xuzhou 221002, China.
AD  - Key Laboratory of Bone Marrow Stem Cell, Jiangsu Province, Xuzhou 221002, China.
FAU - Huang, Yujin
AU  - Huang Y
AD  - Key Laboratory of Bone Marrow Stem Cell, Jiangsu Province, Xuzhou 221002, China.
FAU - Xia, Yuan
AU  - Xia Y
AD  - Key Laboratory of Bone Marrow Stem Cell, Jiangsu Province, Xuzhou 221002, China.
FAU - Chu, Peipei
AU  - Chu P
AD  - Key Laboratory of Bone Marrow Stem Cell, Jiangsu Province, Xuzhou 221002, China.
FAU - Yao, Haina
AU  - Yao H
AD  - Key Laboratory of Bone Marrow Stem Cell, Jiangsu Province, Xuzhou 221002, China.
FAU - Xu, Linyan
AU  - Xu L
AD  - Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, 
      Xuzhou 221002, China.
AD  - Key Laboratory of Bone Marrow Stem Cell, Jiangsu Province, Xuzhou 221002, China.
FAU - Qi, Kunming
AU  - Qi K
AD  - Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, 
      Xuzhou 221002, China.
FAU - Liu, Yun
AU  - Liu Y
AD  - Key Laboratory of Bone Marrow Stem Cell, Jiangsu Province, Xuzhou 221002, China.
FAU - Xu, Kailin
AU  - Xu K
AD  - Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, 
      Xuzhou 221002, China.
AD  - Key Laboratory of Bone Marrow Stem Cell, Jiangsu Province, Xuzhou 221002, China.
FAU - Zeng, Lingyu
AU  - Zeng L
AD  - Department of Hematology, the Affiliated Hospital of Xuzhou Medical College, 
      Xuzhou 221002, China.
AD  - Key Laboratory of Bone Marrow Stem Cell, Jiangsu Province, Xuzhou 221002, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151204
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Carrier Proteins)
RN  - 0 (HMGB1 Protein)
RN  - 0 (HMGB1 protein, mouse)
RN  - 0 (Inflammasomes)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Nlrp3 protein, mouse)
RN  - 0 (Reactive Oxygen Species)
RN  - 8L70Q75FXE (Adenosine Triphosphate)
RN  - G1LN9045DK (Busulfan)
SB  - IM
MH  - Adenosine Triphosphate/metabolism
MH  - Animals
MH  - Busulfan/toxicity
MH  - Carrier Proteins/*biosynthesis/genetics
MH  - Chemical and Drug Induced Liver Injury/*genetics/metabolism/pathology
MH  - Disease Models, Animal
MH  - HMGB1 Protein/biosynthesis/genetics
MH  - Hematopoietic Stem Cell Transplantation/*adverse effects
MH  - Humans
MH  - Inflammasomes/genetics/metabolism
MH  - Inflammation/chemically induced/*genetics/pathology
MH  - Liver/drug effects/injuries/*metabolism/pathology
MH  - Mice
MH  - NLR Family, Pyrin Domain-Containing 3 Protein
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction
PMC - PMC4669461
EDAT- 2015/12/05 06:00
MHDA- 2016/10/08 06:00
PMCR- 2015/12/04
CRDT- 2015/12/05 06:00
PHST- 2015/06/10 00:00 [received]
PHST- 2015/11/06 00:00 [accepted]
PHST- 2015/12/05 06:00 [entrez]
PHST- 2015/12/05 06:00 [pubmed]
PHST- 2016/10/08 06:00 [medline]
PHST- 2015/12/04 00:00 [pmc-release]
AID - srep17828 [pii]
AID - 10.1038/srep17828 [doi]
PST - epublish
SO  - Sci Rep. 2015 Dec 4;5:17828. doi: 10.1038/srep17828.