PMID- 26635882
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151204
LR  - 20230119
IS  - 1687-966X (Print)
IS  - 1687-9678 (Electronic)
VI  - 2016
DP  - 2016
TI  - Improvement in Isolation and Identification of Mouse Oogonial Stem Cells.
PG  - 2749461
LID - 10.1155/2016/2749461 [doi]
LID - 2749461
AB  - Female germline stem cells (FGSCs) or oogonial stem cells (OSCs) have the 
      capacity to generate newborn oocytes and thus open a new door to fight ovarian 
      aging and female infertility. However, the production and identification of OSCs 
      are difficult for investigators. Rare amount of these cells in the ovary results 
      in the failure of the acquisition of OSCs. Furthermore, the oocyte formation by 
      OSCs in vivo was usually confirmed using tissue sections by immunofluorescence or 
      immunohistochemistry in previous studies. STO or MEF feeder cells are derived 
      from mouse, not human. In our study, we modified the protocol. The cells were 
      digested from ovaries and cultured for 2-3 days and then were purified by 
      magnetic-activated cell sorting (MACS). The ovaries and fetus of mice injected 
      with EGFP-positive OSCs were prepared and put on the slides to directly visualize 
      oocyte and progeny formation under microscope. Additionally, the human umbilical 
      cord mesenchymal stem cells (hUC-MSCs) were also used as feeder cells to support 
      the proliferation of OSCs. The results showed that all the modified procedures 
      can significantly improve and facilitate the generation and characterization of 
      OSCs, and hUC-MSCs as feeder will be useful for isolation and proliferation of 
      human OSCs avoiding contamination from mouse.
FAU - Lu, Zhiyong
AU  - Lu Z
AD  - Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan, Hubei 
      430030, China ; Hubei Key Laboratory of Embryonic Stem Cell Research, Tai-He 
      Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
FAU - Wu, Meng
AU  - Wu M
AD  - Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan, Hubei 
      430030, China.
FAU - Zhang, Jinjin
AU  - Zhang J
AD  - Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan, Hubei 
      430030, China.
FAU - Xiong, Jiaqiang
AU  - Xiong J
AD  - Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan, Hubei 
      430030, China.
FAU - Cheng, Jing
AU  - Cheng J
AD  - Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan, Hubei 
      430030, China.
FAU - Shen, Wei
AU  - Shen W
AD  - Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan, Hubei 
      430030, China.
FAU - Luo, Aiyue
AU  - Luo A
AD  - Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan, Hubei 
      430030, China.
FAU - Fang, Li
AU  - Fang L
AD  - Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan, Hubei 
      430030, China.
FAU - Wang, Shixuan
AU  - Wang S
AD  - Department of Obstetrics and Gynecology, Tongji Hospital, Tongji Medical College, 
      Huazhong University of Science and Technology, 1095 Jiefang Road, Wuhan, Hubei 
      430030, China.
LA  - eng
PT  - Journal Article
DEP - 20151109
PL  - United States
TA  - Stem Cells Int
JT  - Stem cells international
JID - 101535822
PMC - PMC4655301
EDAT- 2015/12/05 06:00
MHDA- 2015/12/05 06:01
PMCR- 2015/11/09
CRDT- 2015/12/05 06:00
PHST- 2015/04/13 00:00 [received]
PHST- 2015/06/19 00:00 [revised]
PHST- 2015/06/22 00:00 [accepted]
PHST- 2015/12/05 06:00 [entrez]
PHST- 2015/12/05 06:00 [pubmed]
PHST- 2015/12/05 06:01 [medline]
PHST- 2015/11/09 00:00 [pmc-release]
AID - 10.1155/2016/2749461 [doi]
PST - ppublish
SO  - Stem Cells Int. 2016;2016:2749461. doi: 10.1155/2016/2749461. Epub 2015 Nov 9.