PMID- 26639580
OWN - NLM
STAT- MEDLINE
DCOM- 20161006
LR  - 20181113
IS  - 1347-4405 (Electronic)
IS  - 1342-6311 (Print)
IS  - 1342-6311 (Linking)
VI  - 30
IP  - 4
DP  - 2015
TI  - Identification and Detection of Prokaryotic Symbionts in the Ciliate Metopus from 
      Anaerobic Granular Sludge.
PG  - 335-8
LID - 10.1264/jsme2.ME15154 [doi]
AB  - The aim of the present study was to investigate the prokaryotic community 
      structure of the anaerobic ciliate, Metopus sp. using rRNA sequencing, 
      fluorescence in situ hybridization (FISH), and transmission electron microscopy 
      (TEM). Metopus sp. was physically separated from anaerobic granular sludge in a 
      domestic wastewater treatment plant and anoxically cultivated for 7 d. 16S rRNA 
      gene sequences from the prokaryotes Methanoregula boonei and Clostridium 
      aminobutyricum were abundantly detected in Metopus ciliates. The FISH analysis 
      using the oligonucleotide probes Mg1200b and Cla568 demonstrated that these 
      prokaryotes were localized within Metopus cells. These results identify M. 
      boonei- and C. aminobutyricum-like prokaryotes as novel endosymbionts of Metopus 
      ciliates.
FAU - Hirakata, Yuga
AU  - Hirakata Y
AD  - Department of Civil Engineering, National Institute of Technology, Nagaoka 
      College.
FAU - Oshiki, Mamoru
AU  - Oshiki M
FAU - Kuroda, Kyohei
AU  - Kuroda K
FAU - Hatamoto, Masashi
AU  - Hatamoto M
FAU - Kubota, Kengo
AU  - Kubota K
FAU - Yamaguchi, Takashi
AU  - Yamaguchi T
FAU - Harada, Hideki
AU  - Harada H
FAU - Araki, Nobuo
AU  - Araki N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151204
PL  - Japan
TA  - Microbes Environ
JT  - Microbes and environments
JID - 9710937
RN  - 0 (DNA, Archaeal)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 0 (Sewage)
SB  - IM
MH  - *Biota
MH  - Ciliophora/*microbiology
MH  - Clostridium/classification/genetics/*isolation & purification
MH  - Cluster Analysis
MH  - DNA, Archaeal/chemistry/genetics
MH  - DNA, Bacterial/chemistry/genetics
MH  - DNA, Ribosomal/chemistry/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Methanomicrobiales/classification/genetics/*isolation & purification
MH  - Microbial Consortia
MH  - Microscopy, Electron, Transmission
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Ribosomal, 16S/genetics
MH  - Sequence Analysis, DNA
MH  - Sewage/*microbiology
MH  - *Symbiosis
PMC - PMC4676557
EDAT- 2015/12/08 06:00
MHDA- 2016/10/08 06:00
PMCR- 2015/12/01
CRDT- 2015/12/08 06:00
PHST- 2015/12/08 06:00 [entrez]
PHST- 2015/12/08 06:00 [pubmed]
PHST- 2016/10/08 06:00 [medline]
PHST- 2015/12/01 00:00 [pmc-release]
AID - 30_335 [pii]
AID - 10.1264/jsme2.ME15154 [doi]
PST - ppublish
SO  - Microbes Environ. 2015;30(4):335-8. doi: 10.1264/jsme2.ME15154. Epub 2015 Dec 4.