PMID- 26640335 OWN - NLM STAT- MEDLINE DCOM- 20161107 LR - 20221207 IS - 2219-2840 (Electronic) IS - 1007-9327 (Print) IS - 1007-9327 (Linking) VI - 21 IP - 44 DP - 2015 Nov 28 TI - Potential effect of chronic Helicobacter pylori infection on glucose metabolism of Mongolian gerbils. PG - 12593-604 LID - 10.3748/wjg.v21.i44.12593 [doi] AB - AIM: To assess the effect of Helicobacter pylori (H. pylori) infection on metabolic parameters in Mongolian gerbils. METHODS: A total of 40 male, 5- to 8-wk-old, specific-pathogen-free Mongolian gerbils (30-50 g) were randomly allocated into two groups: a control group (n = 20) and an H. pylori group (n = 20). After a two-week acclimation period, the control group was administered Brucella broth and the H. pylori group was challenged intra-gastrically five times every other day with approximately 10(9)/CFU H. pylori ATCC43504 (CagA+, VacA+). Each group was then divided into two subgroups, which were sacrificed at either 6 or 12 mo. The control and H. pylori subgroups each contained 10 Mongolian gerbils. Body weight, abdominal circumference, and body length were measured, and body mass index (BMI) and Lee's index were calculated. Biochemical assays were used to detect serum indexes, including glucose, glycated hemoglobin (GHb), glycated hemoglobin A1c (HbA1c), triacylglycerol, and total cholesterol, using an automatic biochemistry analyzer. Inflammatory cytokines, including interleukin (IL)-1beta, IL-2, IL-4, IL-10, IL-12, tumor necrosis factor-alpha (TNF-alpha) and interferon (IFN)-gamma, were assayed using ELISA. The expression of insulin and insulin-like growth factor 1 (IGF-1) was detected by immunohistochemistry, and islet apoptosis was measured using the terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL) assay. RESULTS: At each time point, body weight, abdominal circumference, BMI, and Lee's index were increased after H. pylori infection. However, these differences were not significant. H. pylori infection significantly increased the GHb (5.45 +/- 0.53 vs 4.98 +/- 0.22, P < 0.05) and HbA1c (4.91 +/- 0.61 vs 4.61 +/- 0.15, P < 0.05) levels at 12 mo. We observed no significant differences in serum biochemical indexes, including fasting blood glucose, triacylglycerol and total cholesterol, at 6 or 12 mo after infection. H. pylori infection significantly increased the expression of IGF-1 (P < 0.05). Insulin levels from the pancreas and the apoptotic rate of islet beta-cells remained unchanged. Also, we observed no significant differences among cytokines levels, including IL-1beta, IL-2, IL-4, IL-10, IL-12, TNF-alpha and IFN-gamma. IL-4 was the only exception, which increased at 6 (44.36 +/- 25.17 vs 17.38 +/- 3.47, P < 0.05) and 12 mo (33.41 +/- 10.00 vs 18.91 +/- 5.31, P < 0.05) after H. pylori infection. CONCLUSION: Long-term H. pylori infection is significantly associated with high levels of HbA1c in Mongolian gerbils, indicating a potential role of H. pylori infection in glucose dysregulation. FAU - Yang, Zhen AU - Yang Z AD - Zhen Yang, Wei Li, Cong He, Chuan Xie, Yin Zhu, Nong-Hua Lu, Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China. FAU - Li, Wei AU - Li W AD - Zhen Yang, Wei Li, Cong He, Chuan Xie, Yin Zhu, Nong-Hua Lu, Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China. FAU - He, Cong AU - He C AD - Zhen Yang, Wei Li, Cong He, Chuan Xie, Yin Zhu, Nong-Hua Lu, Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China. FAU - Xie, Chuan AU - Xie C AD - Zhen Yang, Wei Li, Cong He, Chuan Xie, Yin Zhu, Nong-Hua Lu, Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China. FAU - Zhu, Yin AU - Zhu Y AD - Zhen Yang, Wei Li, Cong He, Chuan Xie, Yin Zhu, Nong-Hua Lu, Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China. FAU - Lu, Nong-Hua AU - Lu NH AD - Zhen Yang, Wei Li, Cong He, Chuan Xie, Yin Zhu, Nong-Hua Lu, Department of Gastroenterology, The First Affiliated Hospital of Nanchang University, Nanchang 330006, Jiangxi Province, China. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - United States TA - World J Gastroenterol JT - World journal of gastroenterology JID - 100883448 RN - 0 (Biomarkers) RN - 0 (Blood Glucose) RN - 0 (Cytokines) RN - 0 (Glycated Hemoglobin A) RN - 0 (Inflammation Mediators) RN - 0 (Insulin) RN - 0 (Triglycerides) RN - 67763-96-6 (Insulin-Like Growth Factor I) RN - 97C5T2UQ7J (Cholesterol) SB - IM MH - Animals MH - Apoptosis MH - Biomarkers/blood MH - Blood Glucose/*metabolism MH - Cholesterol/blood MH - Chronic Disease MH - Cytokines/blood MH - Disease Models, Animal MH - Gerbillinae MH - Glucose Metabolism Disorders/blood/*microbiology/pathology MH - Glycated Hemoglobin/*metabolism MH - Helicobacter Infections/complications/metabolism/*microbiology/pathology MH - Helicobacter pylori/*pathogenicity MH - Inflammation Mediators/blood MH - Insulin/metabolism MH - Insulin-Like Growth Factor I/metabolism MH - Insulin-Secreting Cells/*metabolism/microbiology/pathology MH - Male MH - Time Factors MH - Triglycerides/blood PMC - PMC4658613 OTO - NOTNLM OT - Glucose metabolism OT - Glycated hemoglobin A1c OT - Helicobacter pylori OT - Inflammatory cytokines EDAT- 2015/12/08 06:00 MHDA- 2016/11/08 06:00 PMCR- 2015/11/28 CRDT- 2015/12/08 06:00 PHST- 2015/04/16 00:00 [received] PHST- 2015/06/19 00:00 [revised] PHST- 2015/09/13 00:00 [accepted] PHST- 2015/12/08 06:00 [entrez] PHST- 2015/12/08 06:00 [pubmed] PHST- 2016/11/08 06:00 [medline] PHST- 2015/11/28 00:00 [pmc-release] AID - 10.3748/wjg.v21.i44.12593 [doi] PST - ppublish SO - World J Gastroenterol. 2015 Nov 28;21(44):12593-604. doi: 10.3748/wjg.v21.i44.12593.