PMID- 26640571
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200929
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 10
IP  - 5
DP  - 2015 Nov
TI  - Protective effects of Tougu Xiaotong capsule on tumor necrosis factor-alpha-injured 
      UMR-106 cells.
PG  - 1908-1914
AB  - Tumor necrosis factor-alpha (TNF-alpha) plays an important role in the abnormal 
      metabolism of osteoblasts (OBs), which leads to subchondral bone (SB) alterations 
      in osteoarthritis. In the present study, Tougu Xiaotong capsule (TXC), a 
      traditional Chinese medicine, was used to treat TNF-alpha-injured OB-like cells. The 
      cellular viability, mortality and ultramicroscopic morphology were evaluated. 
      Thereafter, the activity of alkaline phosphatase (ALP), secretion of osteocalcin 
      (OCN) and mineralization of nodules were analyzed. The results showed that TXC 
      treatment significantly promoted cell proliferation, reduced cellular mortality 
      and improved cellular ultrastructure, particularly that of the endoplasmic 
      reticulum and nucleus. These data indicate that TXC is able to promote cell 
      growth, as well as prevent inflammation in OB-like cells. Furthermore, the 
      activity of ALP, secretion of OCN and mineralization of nodules were accelerated, 
      and the calcium content of the TNF-alpha-injured OB-like cells was promoted by TXC 
      treatment. These results indicate that TXC protected the OB-like cells from 
      TNF-alpha-induced injuries. This may be a potential mechanism through which TXC 
      regulates SB remodeling in the clinical treatment of osteoarthritis.
FAU - Liao, Naishun
AU  - Liao N
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China.
FAU - Huang, Yunmei
AU  - Huang Y
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China ; Fujian Key Laboratory of 
      Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China ; National Laboratory of Traditional 
      Chinese Medicine Pharmacology (Cell Structure and Function), Fujian Academy of 
      Integrative Medicine, Fuzhou, Fujian 350122, P.R. China.
FAU - Ye, Jinxia
AU  - Ye J
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China ; Fujian Key Laboratory of 
      Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China.
FAU - Chen, Wenlie
AU  - Chen W
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China ; Fujian Key Laboratory of 
      Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China ; National Laboratory of Traditional 
      Chinese Medicine Pharmacology (Cell Structure and Function), Fujian Academy of 
      Integrative Medicine, Fuzhou, Fujian 350122, P.R. China.
FAU - Li, Zuan Fang
AU  - Li ZF
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China ; Fujian Key Laboratory of 
      Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China ; National Laboratory of Traditional 
      Chinese Medicine Pharmacology (Cell Structure and Function), Fujian Academy of 
      Integrative Medicine, Fuzhou, Fujian 350122, P.R. China.
FAU - Lin, Ruhui
AU  - Lin R
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China ; Fujian Key Laboratory of 
      Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China ; National Laboratory of Traditional 
      Chinese Medicine Pharmacology (Cell Structure and Function), Fujian Academy of 
      Integrative Medicine, Fuzhou, Fujian 350122, P.R. China.
FAU - Li, Xihai
AU  - Li X
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China.
FAU - Zheng, Liangpu
AU  - Zheng L
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China ; Fujian Key Laboratory of 
      Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China.
FAU - Liu, Xianxiang
AU  - Liu X
AD  - Academy of Integrative Medicine, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China ; Fujian Key Laboratory of 
      Integrative Medicine on Geriatrics, Fujian University of Traditional Chinese 
      Medicine, Fuzhou, Fujian 350122, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20150910
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC4665338
OTO - NOTNLM
OT  - Tougu Xiaotong capsule
OT  - differentiation
OT  - osteoblast
OT  - proliferation
OT  - tumor necrosis factor-alpha
EDAT- 2015/12/08 06:00
MHDA- 2015/12/08 06:01
PMCR- 2015/09/10
CRDT- 2015/12/08 06:00
PHST- 2014/09/26 00:00 [received]
PHST- 2015/08/05 00:00 [accepted]
PHST- 2015/12/08 06:00 [entrez]
PHST- 2015/12/08 06:00 [pubmed]
PHST- 2015/12/08 06:01 [medline]
PHST- 2015/09/10 00:00 [pmc-release]
AID - ETM-0-0-2739 [pii]
AID - 10.3892/etm.2015.2739 [doi]
PST - ppublish
SO  - Exp Ther Med. 2015 Nov;10(5):1908-1914. doi: 10.3892/etm.2015.2739. Epub 2015 Sep 
      10.