PMID- 26641462
OWN - NLM
STAT- MEDLINE
DCOM- 20160506
LR  - 20220316
IS  - 1423-0097 (Electronic)
IS  - 1018-2438 (Linking)
VI  - 168
IP  - 2
DP  - 2015
TI  - The Role of Toll-Like Receptors and Myeloid Differentiation Factor 88 in 
      Bjerkandera adusta-Induced Lung Inflammation.
PG  - 96-106
LID - 10.1159/000441895 [doi]
AB  - BACKGROUND: Recently, a cluster of patients with an intractable allergic fungal 
      cough who were characterized by sensitization to Bjerkandera adusta was reported. 
      In the present study, the role of Toll-like receptors and myeloid differentiation 
      factor 88 (MyD88) in B. adusta-induced lung inflammation was investigated. 
      METHODS: Wild-type (WT), TLR2-/-,TLR4-/-, and MyD88-/- BALB/c mice were 
      intratracheally challenged with B. adusta 4 times at 2-week intervals. Lung 
      pathology, bronchoalveolar lavage fluid (BALF) cytological profiles, and 
      inflammatory mediators in BALF were investigated. Bone marrow-derived macrophages 
      (BMDM) from TLR2-/-,TLR4-/-, TLR2/4-/-, TLR7/9-/-,MyD88-/-, and WT C57BL/6J mice 
      were stimulated with B. adusta for 12 h, and inflammatory mediators in the 
      culture medium were measured. RESULTS: B. adusta caused lung inflammation along 
      with Th2 cytokine [interleukin (IL)-5 and IL-13] and eosinophil-related chemokine 
      [eotaxin and monocyte chemotactic protein (MCP-3)] production, an increase in 
      eosinophils in BALF, and eosinophil infiltration in the airways in WT and TLR4-/- 
      mice. However, Th2 and eosinophil-related responses in TLR2-/- and MyD88-/- mice 
      were low or undetectable. The induction of neutrophils and IL-6, IL-12, IL-17A, 
      and MCP-1 in the BALF of MyD88-/- mice was attenuated compared to that in WT 
      mice. The induction of IL-6, TNF-alpha, MCP-1, and macrophage inflammatory protein-1alpha 
      was reduced or undetectable in B. adusta-stimulated BMDM from TLR7/9-/- and 
      MyD88-/- mice compared to WT mice. CONCLUSIONS: These results suggest that TLR2 
      and the adapter protein MyD88 may play an important role in the induction of 
      eosinophils by B. adusta. However, TLR7/9-MyD88 might be important in the 
      induction of neutrophils and the relevant inflammatory mediators, especially 
      IL-17A.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - He, Miao
AU  - He M
AD  - Environment and Non-Communicable Disease Research Center, School of Public 
      Health, China Medical University, Shenyang, China.
FAU - Ichinose, Takamichi
AU  - Ichinose T
FAU - Song, Yuan
AU  - Song Y
FAU - Yoshida, Yasuhiro
AU  - Yoshida Y
FAU - Kobayashi, Fumihisa
AU  - Kobayashi F
FAU - Maki, Teruya
AU  - Maki T
FAU - Yoshida, Seiichi
AU  - Yoshida S
FAU - Takano, Hirohisa
AU  - Takano H
FAU - Shibamoto, Takayuki
AU  - Shibamoto T
FAU - Sun, Guifan
AU  - Sun G
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151208
PL  - Switzerland
TA  - Int Arch Allergy Immunol
JT  - International archives of allergy and immunology
JID - 9211652
RN  - 0 (Cytokines)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (Toll-Like Receptors)
RN  - 0 (beta-Glucans)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology
MH  - Bronchoalveolar Lavage Fluid/cytology/immunology
MH  - Cell Count
MH  - Cells, Cultured
MH  - *Coriolaceae/metabolism
MH  - Cytokines/immunology
MH  - Lipopolysaccharides/metabolism
MH  - Lung/immunology/pathology
MH  - Macrophages/immunology
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mycoses/*immunology/pathology
MH  - Myeloid Differentiation Factor 88/genetics/*immunology
MH  - Pneumonia/*immunology/pathology
MH  - Toll-Like Receptors/genetics/*immunology
MH  - beta-Glucans/metabolism
EDAT- 2015/12/08 06:00
MHDA- 2016/05/07 06:00
CRDT- 2015/12/08 06:00
PHST- 2015/01/23 00:00 [received]
PHST- 2015/10/19 00:00 [accepted]
PHST- 2015/12/08 06:00 [entrez]
PHST- 2015/12/08 06:00 [pubmed]
PHST- 2016/05/07 06:00 [medline]
AID - 000441895 [pii]
AID - 10.1159/000441895 [doi]
PST - ppublish
SO  - Int Arch Allergy Immunol. 2015;168(2):96-106. doi: 10.1159/000441895. Epub 2015 
      Dec 8.