PMID- 26644089
OWN - NLM
STAT- MEDLINE
DCOM- 20161114
LR  - 20171116
IS  - 1532-4281 (Electronic)
IS  - 1079-9893 (Linking)
VI  - 36
IP  - 2
DP  - 2016
TI  - High glucose induces rat mesangial cells proliferation and MCP-1 expression via 
      ROS-mediated activation of NF-kappaB pathway, which is inhibited by eleutheroside E.
PG  - 152-7
LID - 10.3109/10799893.2015.1061002 [doi]
AB  - Glomerular hypertrophy and extracellular matrix accumulation are early features 
      of diabetic nephropathy (DN). High glucose-induced oxidative stress is implicated 
      in the etiology of DN. This study aims to investigate the effect of eleutheroside 
      E (EE) on high glucose mediated rat mesangial cells (MCs) proliferation and 
      monocyte chemoattractant protein-1 (MCP-1) expression and the underlying 
      mechanism. MCs proliferation was assessed by MTT assay. Reactive oxygen species 
      (ROS) level and MCP-1 expression were evaluated by ELISA kit. The protein 
      expression of p47, NF-kappaB p65, p-NF-kappaB p65, IkappaBalpha, p-IkappaBalpha, IKKbeta and p-IKKbeta were 
      determined by Western blot. The results showed that treatment with EE markedly 
      attenuated high glucose induced MCs proliferation and in a dose-dependent manner. 
      Intervention with EE also significantly blocked high glucose induced 
      intracellular ROS production by decreasing NADPH oxidase activity. Meanwhile, EE 
      administration could effectively alleviate the high glucose-stimulated activation 
      of NF-kappaB, the degradation of IkappaBalpha and the expression of MCP-1. These results 
      demonstrate that high glucose enhances MCs proliferation and MCP-1 expression by 
      activating the ROS/NF-kappaB pathway and can be inhibited by EE. Our findings provide 
      a new perspective for the clinical treatment of DN.
FAU - Yang, Xiuqin
AU  - Yang X
AD  - a Department of Nephrology , Linyi People's Hospital , Linyi , P.R. China .
FAU - Wang, Yangang
AU  - Wang Y
AD  - b Department of Endocrinology and Metabolism , The Affiliated Hospital of Qingdao 
      University , Qingdao , P.R. China , and.
FAU - Gao, Guanqi
AU  - Gao G
AD  - c Department of Endocrinology , Linyi People's Hospital , Linyi , P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20151207
PL  - England
TA  - J Recept Signal Transduct Res
JT  - Journal of receptor and signal transduction research
JID - 9509432
RN  - 0 (Ccl2 protein, rat)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Glucosides)
RN  - 0 (I-kappa B Proteins)
RN  - 0 (Lignans)
RN  - 0 (NF-kappa B)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Transcription Factor RelA)
RN  - 139874-52-5 (NF-KappaB Inhibitor alpha)
RN  - 8JP2P44H3Z (eleutheroside E)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Cell Proliferation/drug effects
MH  - Chemokine CCL2/*biosynthesis/genetics
MH  - Diabetic Nephropathies/*drug therapy/genetics/pathology
MH  - Disease Models, Animal
MH  - Gene Expression Regulation/drug effects
MH  - Glucose/administration & dosage
MH  - Glucosides/*administration & dosage
MH  - Humans
MH  - I-kappa B Proteins/biosynthesis/genetics
MH  - Lignans/*administration & dosage
MH  - Mesangial Cells/*drug effects/metabolism/pathology
MH  - NADPH Oxidases/biosynthesis/genetics
MH  - NF-KappaB Inhibitor alpha
MH  - NF-kappa B/*biosynthesis/genetics
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
MH  - Signal Transduction/drug effects
MH  - Transcription Factor RelA/biosynthesis/genetics
OTO - NOTNLM
OT  - Cell proliferation
OT  - eleutheroside E
OT  - high glucose
OT  - mesangial cells
OT  - monocyte chemoattractant protein-1
OT  - nuclear factor kappa B
OT  - reactive oxygen species
EDAT- 2015/12/09 06:00
MHDA- 2016/11/15 06:00
CRDT- 2015/12/09 06:00
PHST- 2015/12/09 06:00 [entrez]
PHST- 2015/12/09 06:00 [pubmed]
PHST- 2016/11/15 06:00 [medline]
AID - 10.3109/10799893.2015.1061002 [doi]
PST - ppublish
SO  - J Recept Signal Transduct Res. 2016;36(2):152-7. doi: 
      10.3109/10799893.2015.1061002. Epub 2015 Dec 7.