PMID- 26644229
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1365-2826 (Electronic)
IS  - 0953-8194 (Linking)
VI  - 28
IP  - 2
DP  - 2016 Feb
TI  - The Content of Thyroid Hormone Receptor alpha in Ewe Kisspeptin Neurones is not 
      Season-Dependent.
PG  - 12344
LID - 10.1111/jne.12344 [doi]
AB  - Seasonal reproduction is grounded in several mechanisms, among which are 
      plasticity in both hormone synthesis and neuronal networks. Increased daylength 
      on long days (LD) translates into local tri-iodothyronin (T3) production in the 
      mediobasal hypothalamus that will enable the transition to the anoestrus season 
      in sheep. The photoperiod also strongly affects the content of kisspeptin (Kiss), 
      a hypothalamic neuropeptide exerting a potent stimulatory effect on 
      gonadotrophin-releasing hormone release. Our hypothesis was that T3 directly 
      inhibits Kiss release during LD. Using double immunocytochemistry, we first 
      searched for coexpression of thyroid hormone receptor (THR)alpha in Kiss neurones in 
      ewes with an active or inactive gonadotrophic axis. In both the preoptic area and 
      the arcuate nucleus, most Kiss neurones were labelled by THR antibody under both 
      physiological/photoperiodic conditions. These results suggest thyroid hormones 
      may affect Kiss synthesis and release all through the year. We then attempted to 
      assess the influence of T3 on Kiss content in hypothalamic explants sampled from 
      ewes with an active gonadotrophic axis. Kiss produced by hypothalamic explants 
      cultured with different doses of T3 (300 or 600 pg) and subjected to different 
      times of incubation (2 or 24 h) was measured. No significant effects of T3 on 
      Kiss tissular content were observed for the two doses of T3 and for the two 
      incubation times. In light of these findings, potential reasons for the divergent 
      effects of thyroid hormones on Kiss content are discussed. Our data emphasise 
      that the effects of thyroid hormone on Kiss synthesis are not one-sided and may 
      affect a wide range of functions.
CI  - (c) 2015 British Society for Neuroendocrinology.
FAU - Dufourny, L
AU  - Dufourny L
AD  - UMR85 Physiologie de la Reproduction et des Comportements, INRA, F-37380, 
      Nouzilly, France.
AD  - UMR 7247, CNRS, Nouzilly, France.
AD  - Universite de Tours, Tours, France.
AD  - IFCE, Nouzilly, France.
FAU - Gennetay, D
AU  - Gennetay D
AD  - UMR85 Physiologie de la Reproduction et des Comportements, INRA, F-37380, 
      Nouzilly, France.
AD  - UMR 7247, CNRS, Nouzilly, France.
AD  - Universite de Tours, Tours, France.
AD  - IFCE, Nouzilly, France.
FAU - Martinet, S
AU  - Martinet S
AD  - UMR85 Physiologie de la Reproduction et des Comportements, INRA, F-37380, 
      Nouzilly, France.
AD  - UMR 7247, CNRS, Nouzilly, France.
AD  - Universite de Tours, Tours, France.
AD  - IFCE, Nouzilly, France.
FAU - Lomet, D
AU  - Lomet D
AD  - UMR85 Physiologie de la Reproduction et des Comportements, INRA, F-37380, 
      Nouzilly, France.
AD  - UMR 7247, CNRS, Nouzilly, France.
AD  - Universite de Tours, Tours, France.
AD  - IFCE, Nouzilly, France.
FAU - Caraty, A
AU  - Caraty A
AD  - UMR85 Physiologie de la Reproduction et des Comportements, INRA, F-37380, 
      Nouzilly, France.
AD  - UMR 7247, CNRS, Nouzilly, France.
AD  - Universite de Tours, Tours, France.
AD  - IFCE, Nouzilly, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Neuroendocrinol
JT  - Journal of neuroendocrinology
JID - 8913461
RN  - 0 (Kisspeptins)
RN  - 0 (Thyroid Hormone Receptors alpha)
RN  - 06LU7C9H1V (Triiodothyronine)
SB  - IM
MH  - Animals
MH  - Arcuate Nucleus of Hypothalamus/metabolism
MH  - Dose-Response Relationship, Drug
MH  - Female
MH  - Hypothalamus/metabolism
MH  - Kisspeptins/*metabolism
MH  - Neurons/*metabolism
MH  - Preoptic Area/metabolism
MH  - *Seasons
MH  - *Sheep
MH  - Thyroid Hormone Receptors alpha/*metabolism
MH  - Tissue Culture Techniques
MH  - Triiodothyronine/pharmacology
OTO - NOTNLM
OT  - hypothalamic explants
OT  - immunocytochemistry
OT  - seasonal reproduction
OT  - sheep
OT  - triiodothyronin
EDAT- 2015/12/09 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/12/09 06:00
PHST- 2015/08/19 00:00 [received]
PHST- 2015/11/16 00:00 [revised]
PHST- 2015/11/26 00:00 [accepted]
PHST- 2015/12/09 06:00 [entrez]
PHST- 2015/12/09 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1111/jne.12344 [doi]
PST - ppublish
SO  - J Neuroendocrinol. 2016 Feb;28(2):12344. doi: 10.1111/jne.12344.