PMID- 26644454 OWN - NLM STAT- MEDLINE DCOM- 20160512 LR - 20160115 IS - 1470-7926 (Electronic) IS - 1351-0711 (Linking) VI - 73 IP - 2 DP - 2016 Feb TI - Markers of oxidative damage of nucleic acids and proteins among workers exposed to TiO2 (nano) particles. PG - 110-8 LID - 10.1136/oemed-2015-103161 [doi] AB - OBJECTIVE: The use of nanotechnology is growing enormously and occupational physicians have an increasing interest in evaluating potential hazards and finding biomarkers of effect in workers exposed to nanoparticles. METHODS: A study was carried out with 36 workers exposed to (nano)TiO2 pigment and 45 controls. Condensate (EBC) titanium and markers of oxidation of nucleic acids (including 8-hydroxy-2-deoxyguanosine (8-OHdG), 8-hydroxyguanosine (8-OHG), 5-hydroxymethyl uracil (5-OHMeU)) and proteins (such as o-tyrosine (o-Tyr), 3-chlorotyrosine (3-ClTyr) and 3-nitrotyrosine (3-NOTyr)) were analysed from samples of their exhaled breath. RESULTS: In the production workshops, the median total mass 2012 and 2013 TiO2 concentrations were 0.65 and 0.40 mg/m(3), respectively. The median numbers of concentrations measured by the scanning mobility particle sizer (SMPS) and aerodynamic particle sizer (APS) were 1.98 x 10(4) and 2.32 x 10(4) particles/cm(3), respectively; and about 80% of those particles were smaller than 100 nm in diameter. In the research workspace, lower aerosol concentrations (0.16 mg/m(3) and 1.32 x 10(4) particles/cm(3)) were found. Titanium in the EBC was significantly higher in production workers (p<0.001) than in research workers and unexposed controls. Accordingly, most EBC oxidative stress markers, including in the preshift samples, were higher in production workers than in the two other groups. Multiple regression analysis confirmed an association between the production of TiO2 and the levels of studied biomarkers. CONCLUSIONS: The concentration of titanium in EBC may serve as a direct exposure marker in workers producing TiO2 pigment; the markers of oxidative stress reflect the local biological effect of (nano)TiO2 in the respiratory tract of the exposed workers. CI - Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://www.bmj.com/company/products-services/rights-and-licensing/ FAU - Pelclova, D AU - Pelclova D AD - First Faculty of Medicine, Department of Occupational Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic. FAU - Zdimal, V AU - Zdimal V AD - Institute of Chemical Process Fundamentals of the AS CR, vvi, Prague, Czech Republic. FAU - Fenclova, Z AU - Fenclova Z AD - First Faculty of Medicine, Department of Occupational Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic. FAU - Vlckova, S AU - Vlckova S AD - First Faculty of Medicine, Department of Occupational Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic. FAU - Turci, F AU - Turci F AD - Interdepartmental Centre "G Scansetti" for Studies on Asbestos and Other Toxic Particulates and NIS Interdepartmental Centre for Nanostructured Interfaces and Surfaces, University of Torino, Torino, Italy. FAU - Corazzari, I AU - Corazzari I AD - Interdepartmental Centre "G Scansetti" for Studies on Asbestos and Other Toxic Particulates and NIS Interdepartmental Centre for Nanostructured Interfaces and Surfaces, University of Torino, Torino, Italy. FAU - Kacer, P AU - Kacer P AD - Institute of Chemical Technology, Prague, Czech Republic. FAU - Schwarz, J AU - Schwarz J AD - Institute of Chemical Process Fundamentals of the AS CR, vvi, Prague, Czech Republic. FAU - Zikova, N AU - Zikova N FAU - Makes, O AU - Makes O AD - Institute of Chemical Process Fundamentals of the AS CR, vvi, Prague, Czech Republic Institute of Chemical Process Fundamentals of the AS CR, vvi, Prague, Czech Republic. FAU - Syslova, K AU - Syslova K AD - Institute of Chemical Technology, Prague, Czech Republic. FAU - Komarc, M AU - Komarc M AD - First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Institute of Biophysics and Informatics, Prague, Czech Republic Faculty of Physical Education and Sport, Department of Kinanthropology and Humanities, Charles University in Prague, Prague, Czech Republic. FAU - Belacek, J AU - Belacek J AD - First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Institute of Biophysics and Informatics, Prague, Czech Republic. FAU - Navratil, T AU - Navratil T AD - J Heyrovsky Institute of Physical Chemistry of the AS CR, vvi, Prague, Czech Republic First Faculty of Medicine, Charles University in Prague and General University Hospital in Prague, Institute of Medical Biochemistry and Laboratory Diagnostics, Prague, Czech Republic. FAU - Machajova, M AU - Machajova M AD - Faculty of Health Sciences and Social Work, Department of Public Health, Trnava University, Trnava, Slovakia. FAU - Zakharov, S AU - Zakharov S AD - First Faculty of Medicine, Department of Occupational Medicine, Charles University in Prague and General University Hospital in Prague, Prague, Czech Republic. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20151207 PL - England TA - Occup Environ Med JT - Occupational and environmental medicine JID - 9422759 RN - 0 (Biomarkers) RN - 0 (Nucleic Acids) RN - 0 (Particulate Matter) RN - 0 (Proteins) RN - 15FIX9V2JP (titanium dioxide) RN - D1JT611TNE (Titanium) SB - IM MH - Adult MH - Biomarkers/metabolism MH - Breath Tests MH - DNA Damage MH - Humans MH - Male MH - Middle Aged MH - Nanoparticles/*adverse effects MH - Nucleic Acids/*metabolism MH - Occupational Exposure/*adverse effects MH - Oxidation-Reduction MH - Oxidative Stress/*drug effects MH - Particle Size MH - Particulate Matter/*adverse effects MH - Proteins/*metabolism MH - Titanium/*adverse effects MH - Work MH - Young Adult EDAT- 2015/12/09 06:00 MHDA- 2016/05/14 06:00 CRDT- 2015/12/09 06:00 PHST- 2015/07/02 00:00 [received] PHST- 2015/11/11 00:00 [accepted] PHST- 2015/12/09 06:00 [entrez] PHST- 2015/12/09 06:00 [pubmed] PHST- 2016/05/14 06:00 [medline] AID - oemed-2015-103161 [pii] AID - 10.1136/oemed-2015-103161 [doi] PST - ppublish SO - Occup Environ Med. 2016 Feb;73(2):110-8. doi: 10.1136/oemed-2015-103161. Epub 2015 Dec 7.