PMID- 26645425
OWN - NLM
STAT- MEDLINE
DCOM- 20160919
LR  - 20220316
IS  - 1460-9592 (Electronic)
IS  - 1155-5645 (Linking)
VI  - 26
IP  - 1
DP  - 2016 Jan
TI  - Effects of sevoflurane exposure during late pregnancy on brain development of 
      offspring mice.
PG  - 52-9
LID - 10.1111/pan.12785 [doi]
AB  - BACKGROUND: Exposure to some anesthetic agents during the fetal period has been 
      shown to induce neurodegeneration or learning deficits in animal models. 
      Sevoflurane is one of the most prevalent general anesthetics; however, the 
      influence of sevoflurane at a clinically relevant concentration on the developing 
      fetal brain remains unknown. OBJECTIVE: We investigated whether a single 
      sevoflurane exposure during the fetal period would affect neuronal development 
      and learning/memory ability in mice. METHODS: Pregnant mice at gestational day 17 
      were anesthetized with 1.5% sevoflurane in 50% oxygen for 6 h. Mice in the 
      control group were exposed in 50% oxygen without sevoflurane. Pups of some mice 
      in both groups subsequently were delivered early by cesarean section and whole 
      fetal brains were excised. The rest of the pups were delivered naturally at 
      gestational day 20 and were maintained for 8 weeks. The mRNA expression levels of 
      caspase-3, brain derived neurotrophic factor (BDNF), nerve growth factor (NGF), 
      and LIM kinase-1 (LIMK-1) were measured in fetal whole brain and 8-week-old 
      hippocampus sections. Synaptophysin protein in adult hippocampus was assessed 
      immunochemically. In addition, 8-week-old mice were subjected to the radial maze 
      test. RESULTS: No significant difference between sevoflurane and control groups 
      regarding mRNA expression levels of all targets was seen, nor was there an 
      obvious change in synaptophysin protein expression. The results of the maze test 
      revealed that the each-day performance ratios (the rate of errors) of the 
      sevoflurane group were not altered as compared with the control group. 
      CONCLUSIONS: These results suggest that the exposure during late pregnancy to a 
      clinically relevant concentration of sevoflurane does not affect neuronal 
      development and learning/memory ability of offspring mice.
CI  - (c) 2015 John Wiley & Sons Ltd.
FAU - Suehara, Tomomi
AU  - Suehara T
AD  - Division of Anesthesiology, Kobe University Graduate School of Medicine, Kobe, 
      Hyogo, Japan.
FAU - Morishita, Jun
AU  - Morishita J
AD  - Department of Anesthesiology, Higashiosaka City General Hospital, Higashiosaka, 
      Osaka, Japan.
FAU - Ueki, Masaaki
AU  - Ueki M
AD  - Department of Anesthesiology, Nishiwaki Municipal Hospital, Nishiwaki, Hyogo, 
      Japan.
FAU - Ueno, Masaki
AU  - Ueno M
AD  - Department of Pathology and Host Defense, Faculty of Medicine, Kagawa University, 
      Miki-cho, Kagawa, Japan.
FAU - Maekawa, Nobuhiro
AU  - Maekawa N
AD  - Department of Anesthesiology, Nishiwaki Municipal Hospital, Nishiwaki, Hyogo, 
      Japan.
FAU - Mizobuchi, Satoshi
AU  - Mizobuchi S
AD  - Division of Anesthesiology, Kobe University Graduate School of Medicine, Kobe, 
      Hyogo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151013
PL  - France
TA  - Paediatr Anaesth
JT  - Paediatric anaesthesia
JID - 9206575
RN  - 0 (Anesthetics, Inhalation)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Methyl Ethers)
RN  - 38LVP0K73A (Sevoflurane)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - 9061-61-4 (Nerve Growth Factor)
RN  - EC 2.7.11.1 (Lim Kinases)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Anesthetics, Inhalation/*adverse effects/metabolism
MH  - Animals
MH  - Animals, Newborn
MH  - Brain-Derived Neurotrophic Factor/drug effects/metabolism
MH  - Caspase 3/metabolism
MH  - Female
MH  - Hippocampus/*drug effects/metabolism/physiology
MH  - Lim Kinases/drug effects/metabolism
MH  - Maze Learning/*drug effects/physiology
MH  - Methyl Ethers/*adverse effects/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Models, Animal
MH  - Nerve Growth Factor/drug effects/metabolism
MH  - Pregnancy
MH  - Prenatal Exposure Delayed Effects
MH  - Sevoflurane
OTO - NOTNLM
OT  - developing brain
OT  - fetal mouse
OT  - neurotoxicity
OT  - neurotrophin
OT  - sevoflurane
OT  - synaptophysin
EDAT- 2015/12/10 06:00
MHDA- 2016/09/20 06:00
CRDT- 2015/12/10 06:00
PHST- 2015/08/27 00:00 [accepted]
PHST- 2015/12/10 06:00 [entrez]
PHST- 2015/12/10 06:00 [pubmed]
PHST- 2016/09/20 06:00 [medline]
AID - 10.1111/pan.12785 [doi]
PST - ppublish
SO  - Paediatr Anaesth. 2016 Jan;26(1):52-9. doi: 10.1111/pan.12785. Epub 2015 Oct 13.