PMID- 26650931
OWN - NLM
STAT- MEDLINE
DCOM- 20170224
LR  - 20181202
IS  - 1573-7241 (Electronic)
IS  - 0920-3206 (Linking)
VI  - 30
IP  - 2
DP  - 2016 Apr
TI  - Extracellular Vesicles Derived from Adipose Mesenchymal Stem Cells Regulate the 
      Phenotype of Smooth Muscle Cells to Limit Intimal Hyperplasia.
PG  - 111-8
LID - 10.1007/s10557-015-6630-5 [doi]
AB  - PURPOSE: Extracellular vesicles (EVs) derived from mesenchymal stem cells (MSCs) 
      play important roles in the reduction of inflammation in multiple disease models. 
      However, their role in vein graft (VG) remodeling is undefined. We aimed to 
      investigate the effect of EVs from adipose MSCs (ADMSC-EVs) on VG intimal 
      hyperplasia and to explore the possible mechanisms. METHODS: After generation and 
      characterization of control-EVs and ADMSC-EVs in vitro, we investigated their 
      effect on the proliferation and migration of vascular smooth muscle cells (VSMCs) 
      in vitro. Next, we established a mouse model of VG transplantation. Mice 
      underwent surgery and received control-EVs or ADMSC-EVs by intraperitoneal 
      injection every other day for 20 days. VG remodeling was evaluated after 4 weeks. 
      We also assessed the effect of ADMSC-EVs on macrophage migration and inflammatory 
      cytokine expression. RESULTS: Significant inhibitory effects of ADMSC-EVs on in 
      vitro VSMC proliferation (p < 0.05) and migration (p < 0.05) were observed 
      compared with control-EVs. The extent of intimal hyperplasia was significantly 
      decreased in ADMSC-EV-treated mice compared with control-EV-treated mice 
      (26 +/- 8.4 vs. 45 +/- 9.0 mum, p < 0.05). A reduced presence of macrophages was 
      observed in ADMSC-EV-treated mice (p < 0.05). Significantly decreased expression 
      of inflammatory cytokines interleukin (IL)-6 and monocyte chemoattractant 
      protein-1 (MCP-1) was also found in the ADMSC-EV-treated group (both p < 0.05). 
      In addition, phosphorylation of Akt, Erk1/2, and p38 in VGs was decreased in the 
      ADMSC-EV-treated group. CONCLUSIONS: We demonstrated that ADMSC-EVs exert an 
      inhibitory effect on VG neointima formation by regulating VSMC proliferation and 
      migration, macrophage migration, inflammatory cytokine expression, and the 
      related signaling pathways.
FAU - Liu, Rong
AU  - Liu R
AD  - Department of Cardiology, Shanghai Jiao Tong University Affiliated Sixth People's 
      Hospital, 600 Yishan Rd, Shanghai, 200233, China.
FAU - Shen, Hong
AU  - Shen H
AD  - Department of Cardiology, Shanghai Jiao Tong University Affiliated Sixth People's 
      Hospital, 600 Yishan Rd, Shanghai, 200233, China.
FAU - Ma, Jian
AU  - Ma J
AD  - Department of Cardiology, Shanghai Jiao Tong University Affiliated Sixth People's 
      Hospital, 600 Yishan Rd, Shanghai, 200233, China.
FAU - Sun, Leiqing
AU  - Sun L
AD  - Department of Cardiology, Shanghai Jiao Tong University Affiliated Sixth People's 
      Hospital, 600 Yishan Rd, Shanghai, 200233, China.
FAU - Wei, Meng
AU  - Wei M
AD  - Department of Cardiology, Shanghai Jiao Tong University Affiliated Sixth People's 
      Hospital, 600 Yishan Rd, Shanghai, 200233, China. weimeng6h@126.com.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Cardiovasc Drugs Ther
JT  - Cardiovascular drugs and therapy
JID - 8712220
RN  - 0 (Chemokine CCL2)
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Adipose Tissue/metabolism/*pathology
MH  - Animals
MH  - Cell Movement/physiology
MH  - Cell Proliferation/physiology
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Disease Models, Animal
MH  - Endothelium, Vascular/metabolism/pathology
MH  - Extracellular Vesicles/metabolism/*pathology/physiology
MH  - Humans
MH  - Hyperplasia/metabolism/*pathology
MH  - Inflammation/metabolism/pathology
MH  - Interleukin-6/metabolism
MH  - Male
MH  - Mesenchymal Stem Cells/metabolism/*pathology/physiology
MH  - Muscle, Smooth, Vascular/metabolism/pathology
MH  - Myocytes, Smooth Muscle/metabolism/*pathology
MH  - Neointima/metabolism/pathology
MH  - Phenotype
MH  - Phosphorylation/physiology
MH  - Signal Transduction/physiology
OTO - NOTNLM
OT  - Adipose-derived mesenchymal stem cells
OT  - Extracellular vesicles
OT  - Migration
OT  - Proliferation
OT  - Vascular smooth muscle cells
OT  - Vein grafts
EDAT- 2015/12/10 06:00
MHDA- 2017/02/25 06:00
CRDT- 2015/12/10 06:00
PHST- 2015/12/10 06:00 [entrez]
PHST- 2015/12/10 06:00 [pubmed]
PHST- 2017/02/25 06:00 [medline]
AID - 10.1007/s10557-015-6630-5 [pii]
AID - 10.1007/s10557-015-6630-5 [doi]
PST - ppublish
SO  - Cardiovasc Drugs Ther. 2016 Apr;30(2):111-8. doi: 10.1007/s10557-015-6630-5.