PMID- 26650940
OWN - NLM
STAT- MEDLINE
DCOM- 20170404
LR  - 20220321
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 12
DP  - 2015
TI  - Retinal Arteriolar Morphometry Based on Full Width at Half Maximum Analysis of 
      Spectral-Domain Optical Coherence Tomography Images.
PG  - e0144437
LID - 10.1371/journal.pone.0144437 [doi]
LID - e0144437
AB  - OBJECTIVES: In this study, we develop a microdensitometry method using full width 
      at half maximum (FWHM) analysis of the retinal vascular structure in a 
      spectral-domain optical coherence tomography (SD-OCT) image and present the 
      application of this method in the morphometry of arteriolar changes during 
      hypertension. METHODS: Two raters using manual and FWHM methods measured retinal 
      vessel outer and lumen diameters in SD-OCT images. Inter-rater reproducibility 
      was measured using coefficients of variation (CV), intraclass correlation 
      coefficient and a Bland-Altman plot. OCT images from forty-three eyes of 43 
      hypertensive patients and 40 eyes of 40 controls were analyzed using an FWHM 
      approach; wall thickness, wall cross-sectional area (WCSA) and wall to lumen 
      ratio (WLR) were subsequently calculated. RESULTS: Mean difference in inter-rater 
      agreement ranged from -2.713 to 2.658 mum when using a manual method, and ranged 
      from -0.008 to 0.131 mum when using a FWHM approach. The inter-rater CVs were 
      significantly less for the FWHM approach versus the manual method (P < 0.05). 
      Compared with controls, the wall thickness, WCSA and WLR of retinal arterioles 
      were increased in the hypertensive patients, particular in diabetic hypertensive 
      patients. CONCLUSIONS: The microdensitometry method using a FWHM algorithm 
      markedly improved inter-rater reproducibility of arteriolar morphometric 
      analysis, and SD-OCT may represent a promising noninvasive method for in vivo 
      arteriolar morphometry.
FAU - Tong, Yu Hua
AU  - Tong YH
AD  - Quzhou People's Hospital, Quzhou Central Hospital of Zhejiang Chinese Medical 
      University, Quzhou, Zhejiang Province, China.
FAU - Zhu, Tie Pei
AU  - Zhu TP
AD  - Eye Center of Affiliated Second Hospital, Medical College of Zhejiang University, 
      Hangzhou, Zhejiang Province, China.
FAU - Zhao, Ze Lin
AU  - Zhao ZL
AD  - Eye Hospital of Wenzhou Medical University, Zhejiang Eye Hospital, Wenzhou, 
      Zhejiang Province, China.
FAU - Zhan, Hai Jing
AU  - Zhan HJ
AD  - Quzhou People's Hospital, Quzhou Central Hospital of Zhejiang Chinese Medical 
      University, Quzhou, Zhejiang Province, China.
FAU - Jiang, Fang Zheng
AU  - Jiang FZ
AD  - Quzhou People's Hospital, Quzhou Central Hospital of Zhejiang Chinese Medical 
      University, Quzhou, Zhejiang Province, China.
FAU - Lian, Heng Li
AU  - Lian HL
AD  - Eye Hospital of Wenzhou Medical University, Zhejiang Eye Hospital, Wenzhou, 
      Zhejiang Province, China.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Randomized Controlled Trial
PT  - Research Support, Non-U.S. Gov't
DEP - 20151209
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
SB  - IM
MH  - Aged
MH  - Aged, 80 and over
MH  - Arterioles/*pathology
MH  - Case-Control Studies
MH  - Female
MH  - Follow-Up Studies
MH  - Healthy Volunteers
MH  - Humans
MH  - Hypertension/*complications
MH  - Image Interpretation, Computer-Assisted/*methods
MH  - Male
MH  - Middle Aged
MH  - Optic Disk/blood supply
MH  - Prognosis
MH  - Prospective Studies
MH  - Reproducibility of Results
MH  - Retinal Diseases/*diagnosis/etiology
MH  - Tomography, Optical Coherence/instrumentation/*methods
PMC - PMC4674079
COIS- Competing Interests: The authors have declared that no competing interests exist.
EDAT- 2015/12/10 06:00
MHDA- 2017/04/05 06:00
PMCR- 2015/12/09
CRDT- 2015/12/10 06:00
PHST- 2015/07/09 00:00 [received]
PHST- 2015/11/18 00:00 [accepted]
PHST- 2015/12/10 06:00 [entrez]
PHST- 2015/12/10 06:00 [pubmed]
PHST- 2017/04/05 06:00 [medline]
PHST- 2015/12/09 00:00 [pmc-release]
AID - PONE-D-15-29468 [pii]
AID - 10.1371/journal.pone.0144437 [doi]
PST - epublish
SO  - PLoS One. 2015 Dec 9;10(12):e0144437. doi: 10.1371/journal.pone.0144437. 
      eCollection 2015.