PMID- 26651015
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20181113
IS  - 1931-8405 (Electronic)
IS  - 0889-2229 (Print)
IS  - 0889-2229 (Linking)
VI  - 32
IP  - 2
DP  - 2016 Feb
TI  - Dendritic Cells Enhance HIV Infection of Memory CD4(+) T Cells in Human Lymphoid 
      Tissues.
PG  - 203-10
LID - 10.1089/AID.2015.0235 [doi]
AB  - Dendritic cells (DCs) play a key role in controlling infections by coordinating 
      innate and adaptive immune responses to invading pathogens. Paradoxically, DCs 
      can increase HIV-1 dissemination in vitro by binding and transferring infectious 
      virions to CD4(+) T cells, a process called transinfection. Transinfection has 
      been well characterized in cultured cell lines and circulating primary T cells, 
      but it is unknown whether DCs enhance infection of CD4(+) T cells in vivo. In 
      untreated HIV infection, massive CD4(+) T-cell infection and depletion occur in 
      secondary lymphoid tissues long before decline is evident in the peripheral 
      circulation. To study the role of DCs in HIV infection of lymphoid tissues, we 
      utilized human tonsil tissues, cultured either as tissue blocks or as aggregate 
      suspension cultures, in single-round infection experiments. In these experiments, 
      addition of monocyte-derived DCs (MDDCs) to the cultures increased T-cell 
      infection, particularly in CD4(+) T cells expressing lower levels of HLA-DR. 
      Subset analysis demonstrated that MDDCs increased HIV-1 infection of central and 
      effector memory T-cell populations. Depletion of endogenous myeloid DCs (myDCs) 
      from the cultures decreased memory T-cell infection, and readdition of MDDCs 
      restored infection to predepletion levels. Using an HIV-1 fusion assay, we found 
      that MDDCs equally increased HIV delivery into naive, central, and effector 
      memory T cells in the cultures, whereas predepletion of myDCs reduced fusion into 
      memory T cells. Together, these data suggest that resident myDCs facilitate 
      memory T-cell infection in lymphoid tissues, implicating DC-mediated 
      transinfection in driving HIV dissemination within these tissues in untreated 
      HIV/AIDS.
FAU - Reyes-Rodriguez, Angel L
AU  - Reyes-Rodriguez AL
AD  - 1 Department of Molecular Biology and Microbiology, Case Western Reserve 
      University School of Medicine , Cleveland, Ohio.
FAU - Reuter, Morgan A
AU  - Reuter MA
AD  - 2 Department of Microbiology, University of Pennsylvania , Philadelphia, 
      Pennsylvania.
FAU - McDonald, David
AU  - McDonald D
AD  - 1 Department of Molecular Biology and Microbiology, Case Western Reserve 
      University School of Medicine , Cleveland, Ohio.
LA  - eng
GR  - P30 AI036219/AI/NIAID NIH HHS/United States
GR  - R01-AI087511/AI/NIAID NIH HHS/United States
GR  - P30-AI036219/AI/NIAID NIH HHS/United States
GR  - R01-DE025464/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20160107
PL  - United States
TA  - AIDS Res Hum Retroviruses
JT  - AIDS research and human retroviruses
JID - 8709376
RN  - 0 (HLA-DR Antigens)
SB  - IM
MH  - CD4-Positive T-Lymphocytes/immunology/*virology
MH  - Cell Fusion
MH  - Cells, Cultured
MH  - Dendritic Cells/immunology/*virology
MH  - HIV Infections/immunology/*transmission
MH  - HIV-1/immunology
MH  - HLA-DR Antigens/immunology
MH  - Humans
MH  - Immunologic Memory/immunology
MH  - Lymphocyte Depletion
MH  - Palatine Tonsil/cytology/*virology
PMC - PMC4761827
EDAT- 2015/12/10 06:00
MHDA- 2016/12/15 06:00
PMCR- 2017/02/01
CRDT- 2015/12/10 06:00
PHST- 2015/12/10 06:00 [entrez]
PHST- 2015/12/10 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
PHST- 2017/02/01 00:00 [pmc-release]
AID - 10.1089/aid.2015.0235 [pii]
AID - 10.1089/AID.2015.0235 [doi]
PST - ppublish
SO  - AIDS Res Hum Retroviruses. 2016 Feb;32(2):203-10. doi: 10.1089/AID.2015.0235. 
      Epub 2016 Jan 7.