PMID- 26652375
OWN - NLM
STAT- MEDLINE
DCOM- 20160909
LR  - 20191210
IS  - 1873-0191 (Electronic)
IS  - 0928-4931 (Linking)
VI  - 59
DP  - 2016 Feb
TI  - Adhesion and internalization differences of COM nanocrystals on Vero cells before 
      and after cell damage.
PG  - 286-295
LID - S0928-4931(15)30442-2 [pii]
LID - 10.1016/j.msec.2015.10.012 [doi]
AB  - The adhesion and internalization between African green monkey kidney epithelial 
      (Vero) cells (before and after oxidative damage by hydrogen peroxide) and calcium 
      oxalate monohydrate (COM) nanocrystals (97+/-35nm) were investigated so as to 
      discuss the molecular and cellular mechanism of kidney stone formation. Scanning 
      electron microscope (SEM) was used to observe the Vero-COM nanocrystal adhesion; 
      the nanocrystal-cell adhesion was evaluated by measuring the content of 
      malonaldehyde (MDA), the activity of superoxide dismutase (SOD), the expression 
      level of cell surface osteopontin (OPN) and the change of Zeta potential. 
      Confocal microscopy and flow cytometry were used for the observation and 
      quantitative analysis of crystal internalization. In the process of adhesion, the 
      cell viability and the SOD activity declined, the MDA content, Zeta potential, 
      and the OPN expression level increased. The adhesive capacity of injured Vero was 
      obviously stronger than normal cells; in addition the injured cells promoted the 
      aggregation of COM nanocrystals. The capacity of normal cells to internalize 
      crystals was obviously stronger than that of injured cells. Cell injury increased 
      adhesive sites on cell surface, thereby facilitating the aggregation of COM 
      nanocrystals and their attachment, which results in enhanced risk of calcium 
      oxalate stone formation.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Gan, Qiong-Zhi
AU  - Gan QZ
AD  - Department of chemistry, Jinan University, Guangzhou 510632, China;; Institute of 
      Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, 
      China.
FAU - Sun, Xin-Yuan
AU  - Sun XY
AD  - Department of chemistry, Jinan University, Guangzhou 510632, China;; Institute of 
      Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, 
      China.
FAU - Ouyang, Jian-Ming
AU  - Ouyang JM
AD  - Department of chemistry, Jinan University, Guangzhou 510632, China;; Institute of 
      Biomineralization and Lithiasis Research, Jinan University, Guangzhou 510632, 
      China. Electronic address: toyjm@jnu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151023
PL  - Netherlands
TA  - Mater Sci Eng C Mater Biol Appl
JT  - Materials science & engineering. C, Materials for biological applications
JID - 101484109
RN  - 2612HC57YE (Calcium Oxalate)
SB  - IM
MH  - Animals
MH  - Calcium Oxalate/*metabolism
MH  - Cell Adhesion/physiology
MH  - Chlorocebus aethiops
MH  - Nanoparticles/*chemistry
MH  - Vero Cells
OTO - NOTNLM
OT  - Calcium oxalate monohydrate
OT  - Crystal adhesion
OT  - Crystal internalization
OT  - Oxidative damage
EDAT- 2015/12/15 06:00
MHDA- 2016/09/10 06:00
CRDT- 2015/12/15 06:00
PHST- 2015/07/14 00:00 [received]
PHST- 2015/09/18 00:00 [revised]
PHST- 2015/10/05 00:00 [accepted]
PHST- 2015/12/15 06:00 [entrez]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2016/09/10 06:00 [medline]
AID - S0928-4931(15)30442-2 [pii]
AID - 10.1016/j.msec.2015.10.012 [doi]
PST - ppublish
SO  - Mater Sci Eng C Mater Biol Appl. 2016 Feb;59:286-295. doi: 
      10.1016/j.msec.2015.10.012. Epub 2015 Oct 23.