PMID- 26653981
OWN - NLM
STAT- MEDLINE
DCOM- 20160427
LR  - 20151215
IS  - 1879-3592 (Electronic)
IS  - 1383-5718 (Linking)
VI  - 794
DP  - 2015 Dec
TI  - Construction of a cytogenetic dose-response curve for low-dose range 
      gamma-irradiation in human peripheral blood lymphocytes using three-color FISH.
PG  - 32-8
LID - S1383-5718(15)00263-6 [pii]
LID - 10.1016/j.mrgentox.2015.10.002 [doi]
AB  - In order to estimate biological doses after low-dose ionizing radiation exposure, 
      fluorescence in situ hybridization (FISH) using three differentially colored 
      chromosome painting probes was employed to detect exchange-type chromosome 
      aberrations. A reference dose response curve was constructed using blood samples 
      from a female donor whose lymphocytes consistently exhibited a low frequency of 
      cells at the second mitosis under routine culture conditions. Aberration yields 
      were studied for a total of about 155 thousand metaphases obtained from seven 
      dose-points of gamma irradiations (0, 50, 100, 150, 200, 250 and 300mGy). In situ 
      hybridization was performed using commercially available painting probes for 
      chromosomes 1, 2 and 4. With the aid of an automated image-capturing method, 
      exchange-type aberrations involving painted chromosomes were detected with 
      considerable accuracy and speed. The results on the exchange-type aberrations 
      (dicentrics plus translocations) at the seven dose-points showed a good fit to 
      the linear-quadratic model (y=0.0023+0.0015x+0.0819x(2), P=0.83). A blind test 
      proved the reproducibility of the reference dose-response relationship. In the 
      control experiments using blood samples from another donor, the estimated doses 
      calculated on the basis of the present reference curve were proved to be in good 
      agreement with the actual physical doses applied. The present dose-response curve 
      may serve as a means to assess the individual differences in cytogenetical 
      radio-sensitivities.
CI  - Copyright (c) 2015 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Suto, Yumiko
AU  - Suto Y
AD  - Biodosimetry Research Team, Research Center for Radiation Emergency Medicine, 
      National Institute of Radiological Sciences, Chiba, Japan. Electronic address: 
      y-suto@nirs.go.jp.
FAU - Akiyama, Miho
AU  - Akiyama M
AD  - Biodosimetry Research Team, Research Center for Radiation Emergency Medicine, 
      National Institute of Radiological Sciences, Chiba, Japan.
FAU - Noda, Takashi
AU  - Noda T
AD  - REMAT, National Institute of Radiological Sciences, Chiba, Japan.
FAU - Hirai, Momoki
AU  - Hirai M
AD  - Biodosimetry Research Team, Research Center for Radiation Emergency Medicine, 
      National Institute of Radiological Sciences, Chiba, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151024
PL  - Netherlands
TA  - Mutat Res Genet Toxicol Environ Mutagen
JT  - Mutation research. Genetic toxicology and environmental mutagenesis
JID - 101632149
SB  - IM
MH  - Adult
MH  - Chromosome Aberrations/*radiation effects
MH  - Dose-Response Relationship, Radiation
MH  - Female
MH  - *Gamma Rays
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphocytes/*radiation effects
MH  - Metaphase
MH  - Reproducibility of Results
MH  - Translocation, Genetic
OTO - NOTNLM
OT  - 3-color FISH
OT  - Biodosimetry
OT  - Dose response curve
OT  - Gamma-ray irradiation
OT  - Human peripheral lymphocytes
OT  - Low-dose range
EDAT- 2015/12/15 06:00
MHDA- 2016/04/28 06:00
CRDT- 2015/12/15 06:00
PHST- 2015/08/01 00:00 [received]
PHST- 2015/10/17 00:00 [revised]
PHST- 2015/10/20 00:00 [accepted]
PHST- 2015/12/15 06:00 [entrez]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2016/04/28 06:00 [medline]
AID - S1383-5718(15)00263-6 [pii]
AID - 10.1016/j.mrgentox.2015.10.002 [doi]
PST - ppublish
SO  - Mutat Res Genet Toxicol Environ Mutagen. 2015 Dec;794:32-8. doi: 
      10.1016/j.mrgentox.2015.10.002. Epub 2015 Oct 24.