PMID- 26655910
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 2211-1247 (Electronic)
VI  - 13
IP  - 9
DP  - 2015 Dec 1
TI  - Long-Term Two-Photon Calcium Imaging of Neuronal Populations with Subcellular 
      Resolution in Adult Non-human Primates.
PG  - 1989-99
LID - S2211-1247(15)01239-5 [pii]
LID - 10.1016/j.celrep.2015.10.050 [doi]
AB  - Two-photon imaging with genetically encoded calcium indicators (GECIs) enables 
      long-term observation of neuronal activity in vivo. However, there are very few 
      studies of GECIs in primates. Here, we report a method for long-term imaging of a 
      GECI, GCaMP6f, expressed from adeno-associated virus vectors in cortical neurons 
      of the adult common marmoset (Callithrix jacchus), a small New World primate. We 
      used a tetracycline-inducible expression system to robustly amplify neuronal 
      GCaMP6f expression and up- and downregulate it for more than 100 days. We 
      succeeded in monitoring spontaneous activity not only from hundreds of neurons 
      three-dimensionally distributed in layers 2 and 3 but also from single dendrites 
      and axons in layer 1. Furthermore, we detected selective activities from somata, 
      dendrites, and axons in the somatosensory cortex responding to specific tactile 
      stimuli. Our results provide a way to investigate the organization and plasticity 
      of cortical microcircuits at subcellular resolution in non-human primates.
CI  - Copyright (c) 2015 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Sadakane, Osamu
AU  - Sadakane O
AD  - Division of Brain Biology, National Institute for Basic Biology, Aichi 444-8585, 
      Japan; Department of Basic Biology, The Graduate University for Advanced Studies 
      (Sokendai), Aichi 444-8585, Japan; Laboratory for Molecular Analysis of Higher 
      Brain Function, RIKEN Brain Science Institute, Saitama 351-0198, Japan.
FAU - Masamizu, Yoshito
AU  - Masamizu Y
AD  - Department of Basic Biology, The Graduate University for Advanced Studies 
      (Sokendai), Aichi 444-8585, Japan; Division of Brain Circuits, National Institute 
      for Basic Biology, Aichi 444-8585, Japan.
FAU - Watakabe, Akiya
AU  - Watakabe A
AD  - Division of Brain Biology, National Institute for Basic Biology, Aichi 444-8585, 
      Japan; Department of Basic Biology, The Graduate University for Advanced Studies 
      (Sokendai), Aichi 444-8585, Japan; Laboratory for Molecular Analysis of Higher 
      Brain Function, RIKEN Brain Science Institute, Saitama 351-0198, Japan.
FAU - Terada, Shin-Ichiro
AU  - Terada S
AD  - Division of Brain Circuits, National Institute for Basic Biology, Aichi 444-8585, 
      Japan; Laboratory of Cell Recognition and Pattern Formation, Graduate School of 
      Biostudies, Kyoto University, Kyoto 606-8501, Japan.
FAU - Ohtsuka, Masanari
AU  - Ohtsuka M
AD  - Division of Brain Biology, National Institute for Basic Biology, Aichi 444-8585, 
      Japan; Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Brain 
      Science Institute, Saitama 351-0198, Japan.
FAU - Takaji, Masafumi
AU  - Takaji M
AD  - Division of Brain Biology, National Institute for Basic Biology, Aichi 444-8585, 
      Japan; Laboratory for Molecular Analysis of Higher Brain Function, RIKEN Brain 
      Science Institute, Saitama 351-0198, Japan.
FAU - Mizukami, Hiroaki
AU  - Mizukami H
AD  - Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical 
      University, Tochigi 329-0498, Japan.
FAU - Ozawa, Keiya
AU  - Ozawa K
AD  - Division of Genetic Therapeutics, Center for Molecular Medicine, Jichi Medical 
      University, Tochigi 329-0498, Japan.
FAU - Kawasaki, Hiroshi
AU  - Kawasaki H
AD  - Department of Medical Neuroscience, Graduate School of Medicine, Kanazawa 
      University, Ishikawa 920-8640, Japan.
FAU - Matsuzaki, Masanori
AU  - Matsuzaki M
AD  - Department of Basic Biology, The Graduate University for Advanced Studies 
      (Sokendai), Aichi 444-8585, Japan; Division of Brain Circuits, National Institute 
      for Basic Biology, Aichi 444-8585, Japan. Electronic address: mzakim@nibb.ac.jp.
FAU - Yamamori, Tetsuo
AU  - Yamamori T
AD  - Division of Brain Biology, National Institute for Basic Biology, Aichi 444-8585, 
      Japan; Department of Basic Biology, The Graduate University for Advanced Studies 
      (Sokendai), Aichi 444-8585, Japan; Laboratory for Molecular Analysis of Higher 
      Brain Function, RIKEN Brain Science Institute, Saitama 351-0198, Japan. 
      Electronic address: tetsuo.yamamori@riken.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151119
PL  - United States
TA  - Cell Rep
JT  - Cell reports
JID - 101573691
RN  - 0 (Intracellular Calcium-Sensing Proteins)
RN  - 80168379AG (Doxorubicin)
RN  - F8VB5M810T (Tetracycline)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Axons/metabolism
MH  - Calcium/*metabolism
MH  - Callithrix
MH  - Dendrites/metabolism
MH  - Dependovirus/genetics
MH  - Doxorubicin/toxicity
MH  - Intracellular Calcium-Sensing Proteins/genetics/metabolism
MH  - Microscopy, Fluorescence, Multiphoton
MH  - Neurons/drug effects/*metabolism
MH  - Plasmids/genetics/metabolism
MH  - Promoter Regions, Genetic
MH  - Somatosensory Cortex/drug effects/metabolism
MH  - Tetracycline/pharmacology
EDAT- 2015/12/15 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/12/15 06:00
PHST- 2015/06/18 00:00 [received]
PHST- 2015/08/28 00:00 [revised]
PHST- 2015/10/15 00:00 [accepted]
PHST- 2015/12/15 06:00 [entrez]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - S2211-1247(15)01239-5 [pii]
AID - 10.1016/j.celrep.2015.10.050 [doi]
PST - ppublish
SO  - Cell Rep. 2015 Dec 1;13(9):1989-99. doi: 10.1016/j.celrep.2015.10.050. Epub 2015 
      Nov 19.