PMID- 26656852
OWN - NLM
STAT- MEDLINE
DCOM- 20161013
LR  - 20220316
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 5
DP  - 2015 Dec 10
TI  - A method for reproducible measurements of serum BDNF: comparison of the 
      performance of six commercial assays.
PG  - 17989
LID - 10.1038/srep17989 [doi]
LID - 17989
AB  - Brain-Derived Neurotrophic Factor (BDNF) has attracted increasing interest as 
      potential biomarker to support the diagnosis or monitor the efficacy of therapies 
      in brain disorders. Circulating BDNF can be measured in serum, plasma or whole 
      blood. However, the use of BDNF as biomarker is limited by the poor 
      reproducibility of results, likely due to the variety of methods used for sample 
      collection and BDNF analysis. To overcome these limitations, using sera from 40 
      healthy adults, we compared the performance of five ELISA kits 
      (Aviscera-Bioscience, Biosensis, Millipore-ChemiKine(TM), Promega-Emax((R)), 
      R&D-System-Quantikine((R))) and one multiplexing assay (Millipore-Milliplex((R))). 
      All kits showed 100% sample recovery and comparable range. However, they 
      exhibited very different inter-assay variations from 5% to 20%. Inter-assay 
      variations were higher than those declared by the manufacturers with only one 
      exception which also had the best overall performance. Dot-blot analysis revealed 
      that two kits selectively recognize mature BDNF, while the others reacted with 
      both pro-BDNF and mature BDNF. In conclusion, we identified two assays to obtain 
      reliable measurements of human serum BDNF, suitable for future clinical 
      applications.
FAU - Polacchini, Alessio
AU  - Polacchini A
AD  - Department of Life Sciences, University of Trieste, 34127 Trieste, Italy.
FAU - Metelli, Giuliana
AU  - Metelli G
AD  - Department of Life Sciences, University of Trieste, 34127 Trieste, Italy.
FAU - Francavilla, Ruggiero
AU  - Francavilla R
AD  - Department of Life Sciences, University of Trieste, 34127 Trieste, Italy.
FAU - Baj, Gabriele
AU  - Baj G
AD  - Department of Life Sciences, University of Trieste, 34127 Trieste, Italy.
FAU - Florean, Marina
AU  - Florean M
AD  - Department of Transfusion Medicine, Trieste University Hospital, 34142 Trieste, 
      Italy.
FAU - Mascaretti, Luca Giovanni
AU  - Mascaretti LG
AD  - Department of Transfusion Medicine, Trieste University Hospital, 34142 Trieste, 
      Italy.
FAU - Tongiorgi, Enrico
AU  - Tongiorgi E
AD  - Department of Life Sciences, University of Trieste, 34127 Trieste, Italy.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151210
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Biomarkers)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Reagent Kits, Diagnostic)
SB  - IM
MH  - Adult
MH  - Aged
MH  - *Biomarkers
MH  - Brain-Derived Neurotrophic Factor/*blood
MH  - Enzyme-Linked Immunosorbent Assay/*methods/*standards
MH  - Female
MH  - Healthy Volunteers
MH  - Humans
MH  - Male
MH  - Middle Aged
MH  - Reagent Kits, Diagnostic
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
PMC - PMC4675070
EDAT- 2015/12/15 06:00
MHDA- 2016/10/14 06:00
PMCR- 2015/12/10
CRDT- 2015/12/15 06:00
PHST- 2015/03/30 00:00 [received]
PHST- 2015/11/10 00:00 [accepted]
PHST- 2015/12/15 06:00 [entrez]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2016/10/14 06:00 [medline]
PHST- 2015/12/10 00:00 [pmc-release]
AID - srep17989 [pii]
AID - 10.1038/srep17989 [doi]
PST - epublish
SO  - Sci Rep. 2015 Dec 10;5:17989. doi: 10.1038/srep17989.