PMID- 26656937
OWN - NLM
STAT- MEDLINE
DCOM- 20161006
LR  - 20180816
IS  - 1473-558X (Electronic)
IS  - 0959-4965 (Linking)
VI  - 27
IP  - 2
DP  - 2016 Jan 20
TI  - Effect of controlled release of brain-derived neurotrophic factor and 
      neurotrophin-3 from collagen gel on neural stem cells.
PG  - 116-23
LID - 10.1097/WNR.0000000000000507 [doi]
AB  - This study aimed to examine the effect of controlled release of brain-derived 
      neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) from collagen gel on rat 
      neural stem cells (NSCs). With three groups of collagen gel, BDNF/collagen gel, 
      and NT-3/collagen gel as controls, BDNF and NT-3 were tested in the 
      BDNF-NT-3/collagen gel group at different time points. The enzyme-linked 
      immunosorbent assay results showed that BDNF and NT-3 were steadily released from 
      collagen gels for 10 days. The cell viability test and the bromodeoxyuridine 
      incorporation assay showed that BDNF-NT-3/collagen gel supported the survival and 
      proliferation of NSCs. The results also showed that the length of processes was 
      markedly longer and differentiation percentage from NSCs into neurons was much 
      higher in the BDNF-NT-3/collagen gel group than those in the collagen gel, 
      BDNF/collagen gel, and NT-3/collagen gel groups. These findings suggest that 
      BDNF-NT-3/collagen gel could significantly improve the ability of NSCs 
      proliferation and differentiation.
FAU - Huang, Fei
AU  - Huang F
AD  - aDepartment of Orthopaedics, The First Affiliated Hospital of Anhui Medical 
      University bDepartment of Orthopaedics, The Fourth Affiliated Hospital of Anhui 
      Medical University, Hefei, Anhui, People' s Republic of China.
FAU - Wu, Yunfeng
AU  - Wu Y
FAU - Wang, Hao
AU  - Wang H
FAU - Chang, Jun
AU  - Chang J
FAU - Ma, Guangwen
AU  - Ma G
FAU - Yin, Zongsheng
AU  - Yin Z
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Neuroreport
JT  - Neuroreport
JID - 9100935
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Neurotrophin 3)
RN  - 7171WSG8A2 (BDNF protein, human)
RN  - 9007-34-5 (Collagen)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/pharmacokinetics/*pharmacology
MH  - Cell Differentiation/drug effects
MH  - Cell Proliferation/drug effects
MH  - Cell Survival/drug effects
MH  - Cells, Cultured
MH  - Collagen/*pharmacology
MH  - Female
MH  - Neural Stem Cells/*drug effects
MH  - Neurotrophin 3/pharmacokinetics/*pharmacology
MH  - Phenotype
MH  - Rats
MH  - Rats, Sprague-Dawley
EDAT- 2015/12/15 06:00
MHDA- 2016/10/08 06:00
CRDT- 2015/12/15 06:00
PHST- 2015/12/15 06:00 [entrez]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2016/10/08 06:00 [medline]
AID - 10.1097/WNR.0000000000000507 [doi]
PST - ppublish
SO  - Neuroreport. 2016 Jan 20;27(2):116-23. doi: 10.1097/WNR.0000000000000507.