PMID- 26658377
OWN - NLM
STAT- MEDLINE
DCOM- 20161104
LR  - 20161230
IS  - 1532-0987 (Electronic)
IS  - 0891-3668 (Linking)
VI  - 35
IP  - 3
DP  - 2016 Mar
TI  - Reduced Expression of HLA-DR on Monocytes During Severe Respiratory Syncytial 
      Virus Infections.
PG  - e89-96
LID - 10.1097/INF.0000000000001007 [doi]
AB  - BACKGROUND: Respiratory syncytial virus (RSV) is a common cause of bronchiolitis 
      in infants with a wide spectrum of disease severity. Besides environmental and 
      genetic factors, it is thought that the innate immune system plays a pivotal 
      role. The aim of this study was to investigate the expression of immune receptors 
      on monocytes and the in vitro responsiveness from infants with severe RSV 
      infections. METHODS: Peripheral blood mononuclear cells (PBMCs) from infants with 
      RSV infections were isolated. Classical, intermediate and nonclassical monocytes 
      were immunophenotyped for the expression of CD14, CD16, human leukocyte antigen 
      (HLA)-ABC and HLA-DR. PBMCs were stimulated with lipopolysaccharide to determine 
      the secretion of tumor necrosis factor and interleukin (IL)-10 with enzyme-linked 
      immunosorbent assay. RESULTS: During RSV infection, intermediate monocytes are 
      increased in the peripheral blood, whereas classical and nonclassical monocytes 
      are reduced. The expression of CD14 and HLA-ABC is increased on monocytes, 
      whereas the expression of HLA-DR is suppressed. Low HLA-DR expression is 
      correlated with increased disease severity. PBMCs from infants with severe RSV 
      infections show an impaired IL-10 response in vitro. CONCLUSIONS: Phenotyping 
      subpopulations of monocytes combined with in vitro responsiveness reveals 
      significant differences between nonsevere and severe RSV infections. Reduced 
      HLA-DR expression and impaired IL-10 production in vitro during severe RSV 
      infections indicate that an imbalanced innate immune response may play an 
      important role in disease severity.
FAU - Ahout, Inge M L
AU  - Ahout IM
AD  - From the Laboratory of Pediatric Infectious Diseases, Department of Pediatrics, 
      Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, 
      Nijmegen, The Netherlands.
FAU - Jans, Jop
AU  - Jans J
FAU - Haroutiounian, Lilid
AU  - Haroutiounian L
FAU - Simonetti, Elles R
AU  - Simonetti ER
FAU - van der Gaast-de Jongh, Christa
AU  - van der Gaast-de Jongh C
FAU - Diavatopoulos, Dimitri A
AU  - Diavatopoulos DA
FAU - de Jonge, Marien I
AU  - de Jonge MI
FAU - de Groot, Ronald
AU  - de Groot R
FAU - Ferwerda, Gerben
AU  - Ferwerda G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Pediatr Infect Dis J
JT  - The Pediatric infectious disease journal
JID - 8701858
RN  - 0 (Biomarkers)
RN  - 0 (HLA-DR Antigens)
RN  - 130068-27-8 (Interleukin-10)
SB  - IM
MH  - Biomarkers
MH  - Case-Control Studies
MH  - Cohort Studies
MH  - Female
MH  - *Gene Expression
MH  - HLA-DR Antigens/*genetics/*immunology
MH  - Humans
MH  - Immunophenotyping
MH  - Infant
MH  - Interleukin-10/biosynthesis
MH  - Leukocyte Count
MH  - Leukocytes, Mononuclear/immunology/metabolism
MH  - Male
MH  - Monocytes/*immunology/*metabolism
MH  - Phenotype
MH  - Respiratory Syncytial Virus Infections/diagnosis/*genetics/*immunology/virology
MH  - Respiratory Syncytial Virus, Human/*immunology
MH  - Risk Factors
MH  - Severity of Illness Index
EDAT- 2015/12/15 06:00
MHDA- 2016/11/05 06:00
CRDT- 2015/12/15 06:00
PHST- 2015/12/15 06:00 [entrez]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2016/11/05 06:00 [medline]
AID - 10.1097/INF.0000000000001007 [doi]
PST - ppublish
SO  - Pediatr Infect Dis J. 2016 Mar;35(3):e89-96. doi: 10.1097/INF.0000000000001007.