PMID- 26658710
OWN - NLM
STAT- MEDLINE
DCOM- 20161104
LR  - 20161230
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 94
IP  - 2
DP  - 2016 Feb
TI  - The Effect of D-Aspartate on Spermatogenesis in Mouse Testis.
PG  - 30
LID - 10.1095/biolreprod.115.134692 [doi]
AB  - Spermatogenesis is controlled by hormonal secretions from the hypothalamus and 
      pituitary gland, by factors produced locally in the testis, and by direct 
      interaction between germ cells and Sertoli cells in seminiferous tubules. 
      Although the mammalian testis contains high levels of D-aspartate (D-Asp), and 
      D-Asp is known to stimulate the secretion of testosterone in cultured Leydig 
      cells, its role in testis is unclear. We describe here biochemical, 
      immunohistochemical, and flow cytometric studies designed to elucidate 
      developmental changes in testicular D-Asp levels and the direct effect of D-Asp 
      on germ cells. We found that the concentration of D-Asp in mouse testis increased 
      with growth and that fluctuations in D-Asp levels were controlled in part by its 
      degradative enzyme, D-aspartate oxidase expressed in Sertoli cells. In vitro 
      sperm production studies showed that mitosis in premeiotic germ cells was 
      strongly inhibited by the addition of D-Asp to the culture medium. Moreover, 
      immunohistochemical analysis demonstrated that d-Asp accumulated in the 
      differentiated spermatids, indicating either transport of D-Asp to spermatids or 
      its de novo synthesis in these cells. Such compartmentation seems to prevent 
      premeiotic germ cells in mouse testis from being exposed to the excess amount of 
      D-Asp. In concert, our results indicate that in mouse testis, levels of D-Asp are 
      regulated in a spatiotemporal manner and that D-Asp functions as a modulator of 
      spermatogenesis.
CI  - (c) 2016 by the Society for the Study of Reproduction, Inc.
FAU - Tomita, Keiji
AU  - Tomita K
AD  - Department of Urology, Shiga University of Medical Science, Otsu, Shiga, Japan.
FAU - Tanaka, Hiroyuki
AU  - Tanaka H
AD  - Department of Biochemistry and Molecular Biology, Shiga University of Medical 
      Science, Otsu, Shiga, Japan tanakah@belle.shiga-med.ac.jp.
FAU - Kageyama, Susumu
AU  - Kageyama S
AD  - Department of Urology, Shiga University of Medical Science, Otsu, Shiga, Japan.
FAU - Nagasawa, Masayuki
AU  - Nagasawa M
AD  - Department of Urology, Shiga University of Medical Science, Otsu, Shiga, Japan.
FAU - Wada, Akinori
AU  - Wada A
AD  - Department of Urology, Shiga University of Medical Science, Otsu, Shiga, Japan.
FAU - Murai, Ryosuke
AU  - Murai R
AD  - Department of Urology, Shiga University of Medical Science, Otsu, Shiga, Japan.
FAU - Kobayashi, Kenichi
AU  - Kobayashi K
AD  - Department of Urology, Shiga University of Medical Science, Otsu, Shiga, Japan.
FAU - Hanada, Eiki
AU  - Hanada E
AD  - Department of Urology, Shiga University of Medical Science, Otsu, Shiga, Japan.
FAU - Agata, Yasutoshi
AU  - Agata Y
AD  - Department of Biochemistry and Molecular Biology, Shiga University of Medical 
      Science, Otsu, Shiga, Japan.
FAU - Kawauchi, Akihiro
AU  - Kawauchi A
AD  - Department of Urology, Shiga University of Medical Science, Otsu, Shiga, Japan 
      kawauchi@belle.shiga-med.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151209
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
RN  - 4SR0Q8YD1X (D-Aspartic Acid)
SB  - IM
MH  - Animals
MH  - D-Aspartic Acid/metabolism/*pharmacology
MH  - Male
MH  - Mice
MH  - Sertoli Cells/drug effects/metabolism
MH  - Spermatogenesis/*drug effects/physiology
MH  - Spermatozoa/cytology/*drug effects/metabolism
MH  - Testis/cytology/*drug effects/metabolism
OTO - NOTNLM
OT  - D-aspartate
OT  - Sertoli cell
OT  - spermatogenesis
OT  - testis
EDAT- 2015/12/15 06:00
MHDA- 2016/11/05 06:00
CRDT- 2015/12/15 06:00
PHST- 2015/08/19 00:00 [received]
PHST- 2015/12/07 00:00 [accepted]
PHST- 2015/12/15 06:00 [entrez]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2016/11/05 06:00 [medline]
AID - biolreprod.115.134692 [pii]
AID - 10.1095/biolreprod.115.134692 [doi]
PST - ppublish
SO  - Biol Reprod. 2016 Feb;94(2):30. doi: 10.1095/biolreprod.115.134692. Epub 2015 Dec 
      9.