PMID- 26659122 OWN - NLM STAT- MEDLINE DCOM- 20171013 LR - 20200930 IS - 1863-2661 (Electronic) IS - 1863-2653 (Print) IS - 1863-2653 (Linking) VI - 221 IP - 9 DP - 2016 Dec TI - Alcohol dependence-induced regulation of the proliferation and survival of adult brain progenitors is associated with altered BDNF-TrkB signaling. PG - 4319-4335 AB - Effects of withdrawal from ethanol drinking in chronic intermittent ethanol vapor (CIE)-exposed dependent rats and air-exposed nondependent rats on proliferation and survival of progenitor cells in the hippocampus and the medial prefrontal cortex (mPFC) were investigated. Rats were injected with 5'-Bromo 2-deoxyuridine 72 h post-CIE to measure proliferation (2 h-old cells) and survival (29-day-old cells) of progenitors born during a time-point previously reported to elicit a proliferative burst in the hippocampus. Hippocampal and mPFC brain-derived neurotrophic factor (BDNF) and tropomyosin-related kinase B receptor (TrkB) expression were measured 3 h or 21d post-CIE to evaluate neurotrophic signaling during a time point preceding the proliferative burst and survival of newly born progenitors. CIE rats demonstrated elevated drinking compared to nondependent rats and CIE rats maintained elevated drinking following protracted abstinence. Withdrawal from CIE increased BDNF levels in the hippocampus and mPFC, and subsequently increased proliferation in the hippocampus and mPFC compared to nondependent rats and controls. Protracted abstinence from CIE reduced BDNF expression to control levels, and subsequently reduced neurogenesis compared to controls and nondependent rats in the hippocampus. In the mPFC, protracted abstinence reduced BDNF expression to control levels, whereas increased oligodendrogenesis in dependent rats compared to nondependent rats and controls. These results suggest a novel relationship between BDNF and progenitors in the hippocampus and mPFC, in which increased ethanol drinking may alter hippocampal and cortical function in alcohol dependent subjects by altering the cellular composition of newly born progenitors in the hippocampus and mPFC. FAU - Somkuwar, Sucharita S AU - Somkuwar SS AD - Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, La Jolla, CA, 92037, USA. FAU - Fannon, McKenzie J AU - Fannon MJ AD - Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, La Jolla, CA, 92037, USA. FAU - Staples, Miranda C AU - Staples MC AD - Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, La Jolla, CA, 92037, USA. FAU - Zamora-Martinez, Eva R AU - Zamora-Martinez ER AD - Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, La Jolla, CA, 92037, USA. FAU - Navarro, Alvaro I AU - Navarro AI AD - Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, La Jolla, CA, 92037, USA. FAU - Kim, Airee AU - Kim A AD - Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, La Jolla, CA, 92037, USA. FAU - Quigley, Jacqueline A AU - Quigley JA AD - Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, La Jolla, CA, 92037, USA. FAU - Edwards, Scott AU - Edwards S AD - Department of Physiology and Alcohol and Drug Abuse Center of Excellence, Louisiana State University Health Sciences Center, New Orleans, LA, USA. FAU - Mandyam, Chitra D AU - Mandyam CD AD - Committee on the Neurobiology of Addictive Disorders, The Scripps Research Institute, 10550 North Torrey Pines Road, SP30-2400, La Jolla, CA, 92037, USA. cmandyam@scripps.edu. LA - eng GR - P50 AA006420/AA/NIAAA NIH HHS/United States GR - R01 AA020098/AA/NIAAA NIH HHS/United States GR - K01 DA022473/DA/NIDA NIH HHS/United States GR - T32 AA007456/AA/NIAAA NIH HHS/United States GR - K99 AA020839/AA/NIAAA NIH HHS/United States GR - P60 AA006420/AA/NIAAA NIH HHS/United States GR - R00 AA020839/AA/NIAAA NIH HHS/United States GR - R01 DA034140/DA/NIDA NIH HHS/United States PT - Journal Article DEP - 20151211 PL - Germany TA - Brain Struct Funct JT - Brain structure & function JID - 101282001 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 3K9958V90M (Ethanol) RN - EC 2.7.10.1 (Ntrk2 protein, rat) RN - EC 2.7.10.1 (Receptor, trkB) SB - IM MH - Adult Stem Cells/drug effects/metabolism/*physiology MH - Alcoholism/*metabolism MH - Animals MH - Brain-Derived Neurotrophic Factor/*metabolism MH - Cell Proliferation/drug effects MH - Cell Survival/drug effects MH - Ethanol/*administration & dosage MH - Hippocampus/drug effects/metabolism/*physiology MH - Male MH - Neuroglia/drug effects/metabolism/physiology MH - Neurons/drug effects/metabolism/physiology MH - Prefrontal Cortex/drug effects/metabolism/*physiology MH - Rats MH - Rats, Wistar MH - Receptor, trkB/*metabolism MH - Self Administration MH - Signal Transduction/drug effects MH - Substance Withdrawal Syndrome PMC - PMC4903099 MID - NIHMS744495 OTO - NOTNLM OT - Acute withdrawal OT - BrdU OT - Hippocampus OT - Medial prefrontal cortex OT - Protracted abstinence OT - Self-administration EDAT- 2015/12/15 06:00 MHDA- 2017/10/14 06:00 PMCR- 2017/12/01 CRDT- 2015/12/15 06:00 PHST- 2015/03/10 00:00 [received] PHST- 2015/11/26 00:00 [accepted] PHST- 2015/12/15 06:00 [pubmed] PHST- 2017/10/14 06:00 [medline] PHST- 2015/12/15 06:00 [entrez] PHST- 2017/12/01 00:00 [pmc-release] AID - 10.1007/s00429-015-1163-z [pii] AID - 10.1007/s00429-015-1163-z [doi] PST - ppublish SO - Brain Struct Funct. 2016 Dec;221(9):4319-4335. doi: 10.1007/s00429-015-1163-z. Epub 2015 Dec 11.