PMID- 26659792
OWN - NLM
STAT- MEDLINE
DCOM- 20171020
LR  - 20180214
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1433
DP  - 2016
TI  - Fluorescence In Situ Hybridization with Concomitant Immunofluorescence in Human 
      Pancreas.
PG  - 153-8
LID - 10.1007/7651_2015_288 [doi]
AB  - The ability to identify the presence of non-host cells in human pancreas with 
      concomitant characterization of cell phenotype is particularly important to 
      facilitate studies of transplantation and microchimerism resulted from pregnancy. 
      The steps involved in processing tissue for fluorescence in situ hybridization 
      (FISH) can however remove epitopes that are crucial for immunofluorescence and 
      antigen retrieval strategies for immunofluorescence can negatively influence 
      FISH. We describe a robust method to analyze X/Y chromosome constitution and cell 
      phenotype simultaneously on the same pancreatic tissue section.
FAU - Ye, Jody
AU  - Ye J
AD  - Diabetes and Metabolism Unit, School of Clinical Sciences, Southmead Hospital, 
      University of Bristol, Bristol, UK.
FAU - Gillespie, Kathleen M
AU  - Gillespie KM
AD  - Diabetes and Metabolism Unit, School of Clinical Sciences, Southmead Hospital, 
      University of Bristol, Bristol, UK. K.M.Gillespie@bristol.ac.uk.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - *Chimerism
MH  - *Chromosomes, Human, X
MH  - *Chromosomes, Human, Y
MH  - Fluorescent Antibody Technique/*methods
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Pancreas/cytology/*metabolism
MH  - Phenotype
OTO - NOTNLM
OT  - Fluorescence in situ hybridization
OT  - Microchimerism
OT  - Transplantation
EDAT- 2015/12/15 06:00
MHDA- 2017/10/21 06:00
CRDT- 2015/12/15 06:00
PHST- 2015/12/15 06:00 [entrez]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2017/10/21 06:00 [medline]
AID - 10.1007/7651_2015_288 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2016;1433:153-8. doi: 10.1007/7651_2015_288.