PMID- 26660178
OWN - NLM
STAT- MEDLINE
DCOM- 20161005
LR  - 20161230
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1383
DP  - 2016
TI  - Quantification of 11-Carboxy-Delta-9-Tetrahydrocannabinol (THC-COOH) in Meconium 
      Using Gas Chromatography/Mass Spectrometry (GC/MS).
PG  - 97-103
LID - 10.1007/978-1-4939-3252-8_11 [doi]
AB  - Maternal substance abuse is an ongoing concern and detecting drug use during 
      pregnancy is an important component of neonatal care when drug abuse is 
      suspected. Meconium is the preferred specimen for drug testing because it is 
      easier to collect than neonatal urine and it provides a much broader time frame 
      of drug exposure. We describe a method for quantifying 
      11-carboxy-delta-9-tetrahydrocannabinol (THC-COOH) in meconium. After adding a 
      labeled internal standard (THC-COOH D9) and acetonitrile, samples are sonicated 
      to release both free and conjugated THC-COOH. The acetonitrile/aqueous layer is 
      removed and mixed with a strong base to hydrolyze the conjugated THC-COOH. The 
      samples are then extracted with an organic solvent mixture as part of a sample 
      "cleanup." The organic solvent layer is discarded and the remaining aqueous 
      sample is acidified. Following extraction with a second organic mixture, the 
      organic layer is removed and concentrated to dryness. The resulting residue is 
      converted to a trimethylsilyl (TMS) derivative and analyzed using gas 
      chromatography/mass spectrometry (GC/MS) in selective ion monitoring (SIM) mode.
FAU - Peat, Judy
AU  - Peat J
AD  - Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and 
      Clinics, 2401 Gillham Road, Kansas City, MO, 64108, USA.
FAU - Davis, Brehon
AU  - Davis B
AD  - Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and 
      Clinics, 2401 Gillham Road, Kansas City, MO, 64108, USA.
FAU - Frazee, Clint
AU  - Frazee C
AD  - Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and 
      Clinics, 2401 Gillham Road, Kansas City, MO, 64108, USA.
FAU - Garg, Uttam
AU  - Garg U
AD  - Department of Pathology and Laboratory Medicine, Children's Mercy Hospitals and 
      Clinics, 2401 Gillham Road, Kansas City, MO, 64108, USA. ugarg@cmh.edu.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 16849-44-8 (delta(9-11)-tetrahydrocannabinol)
RN  - 7J8897W37S (Dronabinol)
SB  - IM
MH  - Dronabinol/*analogs & derivatives/analysis
MH  - Female
MH  - Gas Chromatography-Mass Spectrometry/*methods
MH  - Humans
MH  - Infant, Newborn
MH  - Meconium/*chemistry
MH  - Pregnancy
MH  - Substance Abuse Detection/*methods
OTO - NOTNLM
OT  - 11-Carboxy-delta-9-tetrahydrocannabinol
OT  - Carboxy-THC
OT  - Marijuana
OT  - Meconium
OT  - Substance abuse
EDAT- 2015/12/15 06:00
MHDA- 2016/10/07 06:00
CRDT- 2015/12/15 06:00
PHST- 2015/12/15 06:00 [entrez]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2016/10/07 06:00 [medline]
AID - 10.1007/978-1-4939-3252-8_11 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2016;1383:97-103. doi: 10.1007/978-1-4939-3252-8_11.