PMID- 2666067
OWN - NLM
STAT- MEDLINE
DCOM- 19890907
LR  - 20190908
IS  - 0168-8227 (Print)
IS  - 0168-8227 (Linking)
VI  - 7
IP  - 1
DP  - 1989 Jun 20
TI  - IgE antibodies to insulin and related peptides, a result of insulin treatment?
PG  - 41-6
AB  - Anti-insulin immunoglobulin E (IgE) antibodies in sera of insulin-treated 
      diabetic patients were characterized by affinity and concentration, and by 
      binding to proinsulin and insulin A and B chains. The affinities of IgE to 
      insulin scattered between less than 10(7) and 0.6 x 10(9) l/mol, the 
      concentrations between 0.3 and 3.5 ng/ml. All positive sera of type 1 and few of 
      type 2 patients recognized proinsulin, A chain, and B chain with comparable 
      affinities and concentrations. Elevated concentrations of total serum IgE in 12 
      of the 16 anti-insulin IgE-positive sera from insulin-treated patients indicate 
      that these patients are predisposed to allergies. The incidence of elevated 
      levels of total serum IgE in type 1 (17.7%) and in type 2 (7.8%) diabetic 
      patients did not differ from the general population. Reagins to insulin and 
      related antigens were also observed in sera of non-diabetic allergic persons 
      without previous contact with exogenous insulin. The natural occurrence of 
      insulin-specific reagins makes the use of IgE as a marker of antigenicity of 
      insulin questionable. Discrepancies between the insulin-positive 
      radioallergosorbent test (RAST) or skin test and clinical manifestations of 
      insulin allergy exist, because IgE antibodies with low affinities require high 
      concentrations of insulin for binding. Such amounts of insulin may occur at the 
      injection site, but not in the circulation.
FAU - Petersen, K G
AU  - Petersen KG
AD  - Department of Endocrinology, Albert-Ludwigs-Universitat, Freiburg, F.R.G.
FAU - Khalaf, A
AU  - Khalaf A
FAU - Naithani, V
AU  - Naithani V
FAU - Gattner, H
AU  - Gattner H
FAU - Kerp, L
AU  - Kerp L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Ireland
TA  - Diabetes Res Clin Pract
JT  - Diabetes research and clinical practice
JID - 8508335
RN  - 0 (Insulin)
RN  - 0 (Insulin Antibodies)
RN  - 0 (Macromolecular Substances)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 9035-68-1 (Proinsulin)
SB  - IM
MH  - Diabetes Mellitus, Type 1/drug therapy/*immunology
MH  - Humans
MH  - Immunoglobulin E/*analysis
MH  - Insulin/*adverse effects/therapeutic use
MH  - Insulin Antibodies/*analysis
MH  - Macromolecular Substances
MH  - Proinsulin/immunology
MH  - Random Allocation
MH  - Reference Values
EDAT- 1989/06/20 00:00
MHDA- 1989/06/20 00:01
CRDT- 1989/06/20 00:00
PHST- 1989/06/20 00:00 [pubmed]
PHST- 1989/06/20 00:01 [medline]
PHST- 1989/06/20 00:00 [entrez]
AID - 0168-8227(89)90043-0 [pii]
AID - 10.1016/0168-8227(89)90043-0 [doi]
PST - ppublish
SO  - Diabetes Res Clin Pract. 1989 Jun 20;7(1):41-6. doi: 
      10.1016/0168-8227(89)90043-0.