PMID- 26662303
OWN - NLM
STAT- MEDLINE
DCOM- 20170206
LR  - 20200624
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 37
IP  - 5
DP  - 2016 May
TI  - miR-22 inhibits the proliferation, motility, and invasion of human glioblastoma 
      cells by directly targeting SIRT1.
PG  - 6761-8
LID - 10.1007/s13277-015-4575-8 [doi]
AB  - Recently, microRNAs (miRNAs), a kind of small and non-coding RNA, can target the 
      downstream molecules. Increasing evidence demonstrates that miRNAs meditate the 
      onset and progression of a variety of tumors. In the present study, we carried 
      out gene transfection, western blot, and reverse transcription PCR (RT-PCR) to 
      explore the role of miR-22 in glioblastoma tissues and cell lines. Here, we 
      verified that the expression of miR-22 was downregulated in glioblastoma tissues 
      and cells rather than matched non-tumor tissues and normal human astrocyte (NHA) 
      cells (p < 0.001). By contrast, SIRT1 messenger RNA (mRNA) and protein were 
      upregulated in glioblastoma tissues and cells (p < 0.001). In vitro miR-22 mimics 
      interfered with cell proliferation, migration, and invasion of U87 and U251 
      cells. Mechanically, the 3'-untranslated regions (3'-UTRs) of SIRT1 were a direct 
      target of miR-22, leading to the decreased expression of SIRT1 protein in U87 and 
      U251 cells. Meanwhile, miR-22 mimics also inhibited the expression of epidermal 
      growth factor receptor (EGFR) and matrix metallopeptidase 9 (MMP9). In 
      conclusion, miR-22 inhibited cell proliferation, migration, and invasion via 
      targeting the 3'-UTR of SIRT1 in the progression of glioblastoma and miR-22-SIRT1 
      pathway can be recommended as a potential target for treatment of glioblastoma.
FAU - Chen, Hanchun
AU  - Chen H
AD  - Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiao Tong 
      University School of Medicine, Suzhou, Jiangsu, 215021, People's Republic of 
      China.
FAU - Lu, Qiong
AU  - Lu Q
AD  - Department of Laboratory Medicine, Changhai Hospital, Second Military Medical 
      University, Shanghai, 200433, People's Republic of China.
FAU - Fei, Xifeng
AU  - Fei X
AD  - Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiao Tong 
      University School of Medicine, Suzhou, Jiangsu, 215021, People's Republic of 
      China.
FAU - Shen, Likui
AU  - Shen L
AD  - Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiao Tong 
      University School of Medicine, Suzhou, Jiangsu, 215021, People's Republic of 
      China.
FAU - Jiang, Dongyi
AU  - Jiang D
AD  - Department of Neurosurgery, Suzhou Kowloon Hospital, Shanghai Jiao Tong 
      University School of Medicine, Suzhou, Jiangsu, 215021, People's Republic of 
      China.
FAU - Dai, Dongwei
AU  - Dai D
AD  - Department of Neurosurgery, Changhai Hospital, Second Military Medical 
      University, Shanghai, 200433, People's Republic of China. daidwshanghai@163.com.
LA  - eng
PT  - Journal Article
DEP - 20151210
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (3' Untranslated Regions)
RN  - 0 (MIRN22 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - EC 3.5.1.- (Sirtuin 1)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Gene Expression Profiling
MH  - *Gene Expression Regulation, Neoplastic
MH  - Glioblastoma/*genetics/mortality/pathology
MH  - Humans
MH  - MicroRNAs/chemistry/*genetics
MH  - *RNA Interference
MH  - RNA, Messenger/chemistry/genetics
MH  - Sirtuin 1/chemistry/*genetics
MH  - Transcriptome
OTO - NOTNLM
OT  - Glioblastoma
OT  - SIRT1
OT  - miR-22
EDAT- 2015/12/15 06:00
MHDA- 2017/02/07 06:00
CRDT- 2015/12/15 06:00
PHST- 2015/11/14 00:00 [received]
PHST- 2015/12/01 00:00 [accepted]
PHST- 2015/12/15 06:00 [entrez]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2017/02/07 06:00 [medline]
AID - 10.1007/s13277-015-4575-8 [pii]
AID - 10.1007/s13277-015-4575-8 [doi]
PST - ppublish
SO  - Tumour Biol. 2016 May;37(5):6761-8. doi: 10.1007/s13277-015-4575-8. Epub 2015 Dec 
      10.