PMID- 26662640
OWN - NLM
STAT- MEDLINE
DCOM- 20171018
LR  - 20200422
IS  - 1865-1682 (Electronic)
IS  - 1865-1674 (Print)
IS  - 1865-1674 (Linking)
VI  - 64
IP  - 3
DP  - 2017 Jun
TI  - Multiplex PCR and Microarray for Detection of Swine Respiratory Pathogens.
PG  - 834-848
LID - 10.1111/tbed.12449 [doi]
AB  - Porcine respiratory disease complex (PRDC) is one of the most important health 
      concerns for pig producers and can involve multiple viral and bacterial 
      pathogens. No simple, single-reaction diagnostic test currently exists for the 
      simultaneous detection of major pathogens commonly associated with PRDC. 
      Furthermore, the detection of most of the bacterial pathogens implicated in PRDC 
      currently requires time-consuming culture-based methods that can take several 
      days to obtain results. In this study, a novel prototype automated microarray 
      that integrates and automates all steps of post-PCR microarray processing for the 
      simultaneous detection and typing of eight bacteria and viruses commonly 
      associated with PRDC is described along with associated multiplex reverse 
      transcriptase PCR. The user-friendly assay detected and differentiated between 
      four viruses [porcine reproductive and respiratory syndrome virus (PRRSV), 
      influenza A virus, porcine circovirus type 2, porcine respiratory corona virus], 
      four bacteria (Mycoplasma hyopneumoniae, Pasteurella multocida, Salmonella 
      enterica serovar Choleraesuis, Streptococcus suis), and further differentiated 
      between type 1 and type 2 PRRSV as well as toxigenic and non-toxigenic 
      P. multocida. The assay accurately identified and typed a panel of 34 strains 
      representing the eight targeted pathogens and was negative when tested with 34 
      relevant and/or closely related non-target bacterial and viral species. All 
      targets were also identified singly or in combination in a panel of clinical lung 
      samples and/or experimentally inoculated biological material.
CI  - (c) Her Majesty the Queen in Right of Canada 2015.
FAU - Lung, O
AU  - Lung O
AD  - Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food 
      Inspection Agency, Lethbridge, AB, Canada.
FAU - Ohene-Adjei, S
AU  - Ohene-Adjei S
AD  - Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food 
      Inspection Agency, Lethbridge, AB, Canada.
FAU - Buchanan, C
AU  - Buchanan C
AD  - Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food 
      Inspection Agency, Lethbridge, AB, Canada.
FAU - Joseph, T
AU  - Joseph T
AD  - Animal Health Centre, BC Ministry of Agriculture, Abbotsford, BC, Canada.
FAU - King, R
AU  - King R
AD  - Animal Health and Assurance Division, Alberta Agriculture and Rural Development, 
      Edmonton, AB, Canada.
FAU - Erickson, A
AU  - Erickson A
AD  - Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food 
      Inspection Agency, Lethbridge, AB, Canada.
FAU - Detmer, S
AU  - Detmer S
AD  - Department of Veterinary Pathology, Western College of Veterinary Medicine, 
      University of Saskatchewan, Saskatoon, SK, Canada.
FAU - Ambagala, A
AU  - Ambagala A
AD  - Lethbridge Laboratory, National Centres for Animal Disease, Canadian Food 
      Inspection Agency, Lethbridge, AB, Canada.
LA  - eng
PT  - Journal Article
DEP - 20151212
PL  - Germany
TA  - Transbound Emerg Dis
JT  - Transboundary and emerging diseases
JID - 101319538
SB  - IM
MH  - Animals
MH  - Bacteria/classification/*isolation & purification
MH  - Multiplex Polymerase Chain Reaction/*veterinary
MH  - Protein Array Analysis/methods/*veterinary
MH  - Sensitivity and Specificity
MH  - Swine
MH  - Swine Diseases/*diagnosis/microbiology/virology
MH  - Viruses/classification/*isolation & purification
PMC - PMC7169873
OTO - NOTNLM
OT  - microarray
OT  - multiplex PCR
OT  - porcine respiratory disease complex
OT  - swine respiratory disease
COIS- None of the authors of this paper has a financial or personal relationship with 
      other people or organizations that could inappropriately influence or bias the 
      content of the paper.
EDAT- 2015/12/15 06:00
MHDA- 2017/10/19 06:00
PMCR- 2020/04/20
CRDT- 2015/12/15 06:00
PHST- 2015/04/17 00:00 [received]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2017/10/19 06:00 [medline]
PHST- 2015/12/15 06:00 [entrez]
PHST- 2020/04/20 00:00 [pmc-release]
AID - TBED12449 [pii]
AID - 10.1111/tbed.12449 [doi]
PST - ppublish
SO  - Transbound Emerg Dis. 2017 Jun;64(3):834-848. doi: 10.1111/tbed.12449. Epub 2015 
      Dec 12.