PMID- 26663048
OWN - NLM
STAT- MEDLINE
DCOM- 20160523
LR  - 20190221
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 32
IP  - 6
DP  - 2015 Dec
TI  - [Comparison of results of improved FISH and conventional karyotyping analysis of 
      2607 amniotic fluid samples].
PG  - 785-8
LID - 10.3760/cma.j.issn.1003-9406.2015.06.006 [doi]
AB  - OBJECTIVE: To compare the results of fluorescence in situ hybridization (FISH) 
      assay and conventional karyotyping analysis for the detection of chromosomal 
      aneuploidies. METHODS: In total 2607 amniotic fluid samples were subjected to an 
      improved FISH technique. Meanwhile, karyotype analysis was also ordered for each 
      sample. RESULTS: Of the 2607 samples, 62 abnormalities were identified by FISH, 
      which included 62 cases of trisomy 21, 5 cases of 45,X, 12 cases of trisomy 18, 3 
      cases of trisomy 13, and 1 case of 47, XYY. Conventional karyotyping analysis has 
      identified 63 cases of trisomy 21, 5 cases of 45,X, 12 cases of trisomy 18, 3 
      cases of trisomy 13, 1 case of 47, XYY, and 57 cases of balanced translocations. 
      The success rate of FISH detection was 98.4% for trisomy 21, and 100% for 45,X, 
      trisomy 18 and trisomy 13. CONCLUSION: For the detection of chromosomal 
      aneuploidies, FISH assay is quick, simple, accurate and can reduce workload when 
      aminocyte culture has failed. As an auxiliary method for amniocytic analysis, it 
      can provide reference for the consultation of those with advanced age and high 
      pregnancy risk.
FAU - Yang, Yan
AU  - Yang Y
AD  - Prenatal Diagnosis Center of Jiangxi Women and Children's Hospital, Nanchang, 
      Jiangxi 330006 P.R. China. lyq0914@126.com.
FAU - Liu, Yanqiu
AU  - Liu Y
FAU - Huang, Ning
AU  - Huang N
FAU - Xie, Kang
AU  - Xie K
LA  - chi
PT  - Comparative Study
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
SB  - IM
MH  - Adult
MH  - Amniocentesis/*methods
MH  - Amniotic Fluid/cytology/*metabolism
MH  - Chromosomes, Human, Pair 18/genetics
MH  - Chromosomes, Human, Pair 3/genetics
MH  - Chromosomes, Human, Y/genetics
MH  - Down Syndrome/genetics
MH  - Female
MH  - Fetal Diseases/diagnosis/genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Karyotype
MH  - Karyotyping/*methods
MH  - Middle Aged
MH  - Pregnancy
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Sex Chromosome Aberrations
MH  - Trisomy/genetics
MH  - Trisomy 18 Syndrome
MH  - Turner Syndrome/genetics
MH  - Young Adult
EDAT- 2015/12/15 06:00
MHDA- 2016/05/24 06:00
CRDT- 2015/12/15 06:00
PHST- 2015/12/15 06:00 [entrez]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2016/05/24 06:00 [medline]
AID - 940632164 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2015.06.006 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2015 Dec;32(6):785-8. doi: 
      10.3760/cma.j.issn.1003-9406.2015.06.006.