PMID- 26663674
OWN - NLM
STAT- MEDLINE
DCOM- 20171018
LR  - 20180314
IS  - 1552-4957 (Electronic)
IS  - 1552-4949 (Linking)
VI  - 90
IP  - 6
DP  - 2016 Nov
TI  - Analytical and diagnostic validation of a flow cytometric strategy to quantify 
      blood and marrow infiltration in dogs with large B-cell lymphoma.
PG  - 525-530
LID - 10.1002/cyto.b.21353 [doi]
AB  - BACKGROUND: Lymph node (LN), peripheral blood (PB), and bone marrow (BM) samples 
      are commonly analyzed by flow cytometry (FC) for the immunophenotyping and 
      staging of canine lymphomas. A prognostic value for FC BM infiltration in dogs 
      with large B-cell lymphoma (LBCL) was demonstrated. Aim of this study was to 
      define the analytical performances of this technique, and to establish a cutoff 
      suitable to safely discriminate between infiltrated and noninfiltrated PB and BM 
      samples. METHODS: Large B-cells were added to control PB and BM samples, to 
      achieve twelve different large B-cells concentrations, ranging from 0 to 50%. The 
      percentage of large B-cells was recorded for each dilution, using a BD Accuri C6 
      FC. Accuracy was evaluated by Passing-Bablok regression analysis. Intra-assay 
      precision was assessed at 0%, 1, 3, and 10% dilutions evaluating the CVs of 10 
      repeated acquisitions. ROC curves were drawn to identify the cutoffs most 
      suitable to discriminate between 25 infiltrated (PARR-positive) and 25 
      noninfiltrated (PARR-negative) PB and BM samples, respectively. RESULTS: Optimal 
      analytical accuracy and precision were achieved. Almost all CVs were <10%. 
      Negative controls had up to 0.5% large B-cells, with 50 and 22% CV in PB and BM 
      samples, respectively, 0.56 and 2.45% cutoffs were selected based on the ROC 
      curves for PB and BM samples, respectively. CONCLUSIONS: Quantification of large 
      B-cells in PB and BM samples by FC is reliable and analytical performances met 
      the acceptance criteria. Assessment of performances of different instruments and 
      protocols is warranted. (c) 2016 International Clinical Cytometry Society.
CI  - (c) 2016 International Clinical Cytometry Society.
FAU - Riondato, Fulvio
AU  - Riondato F
AD  - Department of Veterinary Sciences, University of Turin, Grugliasco, Turin, Italy.
FAU - Miniscalco, Barbara
AU  - Miniscalco B
AD  - Department of Veterinary Sciences, University of Turin, Grugliasco, Turin, Italy.
FAU - Poggi, Alessia
AU  - Poggi A
AD  - Department of Veterinary Sciences, University of Turin, Grugliasco, Turin, Italy.
FAU - Arico, Arianna
AU  - Arico A
AD  - Department of Comparative Biomedicine and Food Science, University of Padua, 
      Legnaro, Padua, Italy.
FAU - Aresu, Luca
AU  - Aresu L
AD  - Department of Comparative Biomedicine and Food Science, University of Padua, 
      Legnaro, Padua, Italy.
FAU - Comazzi, Stefano
AU  - Comazzi S
AD  - Department of Veterinary Sciences and Public Health, University of Milan, Italy.
FAU - Martini, Valeria
AU  - Martini V
AD  - Department of Veterinary Sciences and Public Health, University of Milan, Italy.
LA  - eng
PT  - Journal Article
DEP - 20160203
PL  - United States
TA  - Cytometry B Clin Cytom
JT  - Cytometry. Part B, Clinical cytometry
JID - 101235690
SB  - IM
MH  - Animals
MH  - B-Lymphocytes/pathology
MH  - Bone Marrow/*pathology
MH  - Dogs
MH  - Flow Cytometry/*methods
MH  - Immunophenotyping/methods
MH  - Lymph Nodes/pathology
MH  - Lymphoma, Large B-Cell, Diffuse/*diagnosis/*pathology
MH  - Prognosis
OTO - NOTNLM
OT  - dog
OT  - flow cytometry
OT  - large B-cell lymphoma
OT  - staging
OT  - validation
EDAT- 2015/12/15 06:00
MHDA- 2017/10/19 06:00
CRDT- 2015/12/15 06:00
PHST- 2015/06/05 00:00 [received]
PHST- 2015/11/10 00:00 [revised]
PHST- 2015/12/09 00:00 [accepted]
PHST- 2015/12/15 06:00 [pubmed]
PHST- 2017/10/19 06:00 [medline]
PHST- 2015/12/15 06:00 [entrez]
AID - 10.1002/cyto.b.21353 [doi]
PST - ppublish
SO  - Cytometry B Clin Cytom. 2016 Nov;90(6):525-530. doi: 10.1002/cyto.b.21353. Epub 
      2016 Feb 3.