PMID- 26667361
OWN - NLM
STAT- MEDLINE
DCOM- 20170403
LR  - 20181113
IS  - 1573-742X (Electronic)
IS  - 0929-5305 (Print)
IS  - 0929-5305 (Linking)
VI  - 42
IP  - 1
DP  - 2016 Jul
TI  - The role of mononuclear cell tissue factor and inflammatory cytokines in patients 
      with chronic thromboembolic pulmonary hypertension.
PG  - 38-45
LID - 10.1007/s11239-015-1323-2 [doi]
AB  - Thrombosis and inflammation are two major factors underlying chronic 
      thromboembolic pulmonary hypertension (CTEPH). Tissue factor (TF), C-reactive 
      protein (CRP), tumor necrosis factor-alpha (TNF-alpha) and monocyte chemoattractant 
      protein 1 (MCP-1) may play critical roles in the process of CTEPH thrombosis and 
      pulmonary vascular remodeling. Ten patients with a confirmed diagnosis of CTEPH, 
      20 patients with acute pulmonary thromboembolism and 15 patients with other types 
      of pulmonary hypertension were enrolled in this study, along with 20 healthy 
      subjects as the control group. The immunoturbidimetric method was used to 
      determine the plasma content of CRP. The plasma levels of TNF-alpha, MCP-1, and TF 
      antigen were measured by an enzyme-linked immunosorbent assay, and TF activity 
      was measured by the chromogenic substrate method. Percoll density gradient 
      centrifugation was used to separate peripheral blood mononuclear cells from 
      plasma. The level of monocyte TF mRNA was examined by reverse 
      transcriptase-polymerase chain reaction. The correlations between all indices 
      described above were analyzed. In CTEPH patients, the expression of CRP, TNF-alpha, 
      and MCP-1 was significantly higher than that in controls (P < 0.05). The levels 
      of TF activity, TF antigen, and TF mRNA in monocyte cells were increased in CTEPH 
      patients when compared with control subjects, but only the TF antigen and TF mRNA 
      levels were significantly different (P < 0.05). In CTEPH patients, levels of CRP, 
      MCP-1, and TNF-alpha significantly correlated with the level of TF antigen in plasma. 
      TF gene expression was increased in patients with CTEPH, suggesting that 
      blood-borne TF mainly comes from mononuclear cells. TF expression significantly 
      correlated with levels of CRP, TNF-alpha and MCP-1. These factors may play an 
      important role in the development of CTEPH via the 
      inflammation-coagulation-thrombosis cycle.
FAU - Yang, Minxia
AU  - Yang M
AD  - Intensive Care Unit, First Affiliated Hospital of Fujian Medical University, 
      Fuzhou, 350005, Fujian Province, China.
FAU - Deng, Chaosheng
AU  - Deng C
AD  - Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of 
      Fujian Medical University, Fuzhou, 350005, Fujian Province, China. 
      jasonsci7333@163.com.
FAU - Wu, Dawen
AU  - Wu D
AD  - Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of 
      Fujian Medical University, Fuzhou, 350005, Fujian Province, China.
FAU - Zhong, Zhanghua
AU  - Zhong Z
AD  - Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of 
      Fujian Medical University, Fuzhou, 350005, Fujian Province, China.
FAU - Lv, Xiaoting
AU  - Lv X
AD  - Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of 
      Fujian Medical University, Fuzhou, 350005, Fujian Province, China.
FAU - Huang, Zhihua
AU  - Huang Z
AD  - Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of 
      Fujian Medical University, Fuzhou, 350005, Fujian Province, China.
FAU - Lian, Ningfang
AU  - Lian N
AD  - Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of 
      Fujian Medical University, Fuzhou, 350005, Fujian Province, China.
FAU - Liu, Kaixiong
AU  - Liu K
AD  - Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of 
      Fujian Medical University, Fuzhou, 350005, Fujian Province, China.
FAU - Zhang, Qiaoxian
AU  - Zhang Q
AD  - Division of Respiratory and Critical Care Medicine, First Affiliated Hospital of 
      Fujian Medical University, Fuzhou, 350005, Fujian Province, China.
LA  - eng
PT  - Journal Article
PL  - Netherlands
TA  - J Thromb Thrombolysis
JT  - Journal of thrombosis and thrombolysis
JID - 9502018
RN  - 0 (Chemokine CCL2)
RN  - 0 (Cytokines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 9007-41-4 (C-Reactive Protein)
RN  - 9035-58-9 (Thromboplastin)
SB  - IM
MH  - C-Reactive Protein/analysis
MH  - Case-Control Studies
MH  - Chemokine CCL2/blood
MH  - Chronic Disease
MH  - Cytokines/immunology/*physiology
MH  - Humans
MH  - Hypertension, Pulmonary/*blood
MH  - Monocytes/metabolism
MH  - Pulmonary Embolism/*blood
MH  - RNA, Messenger/analysis
MH  - Thromboplastin/analysis/genetics/*physiology
MH  - Tumor Necrosis Factor-alpha/blood
PMC - PMC4877417
OTO - NOTNLM
OT  - C-reactive protein
OT  - Chronic thromboembolic pulmonary hypertension
OT  - Monocyte chemoattractant protein-1
OT  - Tissue factor
OT  - Tumor necrosis factor-alpha
EDAT- 2015/12/17 06:00
MHDA- 2017/04/04 06:00
PMCR- 2015/12/14
CRDT- 2015/12/16 06:00
PHST- 2015/12/16 06:00 [entrez]
PHST- 2015/12/17 06:00 [pubmed]
PHST- 2017/04/04 06:00 [medline]
PHST- 2015/12/14 00:00 [pmc-release]
AID - 10.1007/s11239-015-1323-2 [pii]
AID - 1323 [pii]
AID - 10.1007/s11239-015-1323-2 [doi]
PST - ppublish
SO  - J Thromb Thrombolysis. 2016 Jul;42(1):38-45. doi: 10.1007/s11239-015-1323-2.