PMID- 26667767
OWN - NLM
STAT- MEDLINE
DCOM- 20160914
LR  - 20181202
IS  - 1165-158X (Electronic)
IS  - 0145-5680 (Linking)
VI  - 61
IP  - 8
DP  - 2015 Dec 9
TI  - Trans-resveratrol induces a potential anti-lipogenic effect in 
      lipopolysaccharide-stimulated enterocytes.
PG  - 9-16
AB  - A DNA microarray analysis was conducted in Caco-2 cells to analyse the protective 
      effects of trans-resveratrol on enterocyte physiology and metabolism in 
      pro-inflammatory conditions. Cells were pre-treated with 50 muMu of 
      trans-resveratrol and, subsequently, lipopolysaccharide (LPS) was added for 48 h. 
      The microarray analysis revealed 121 genes differentially expressed between 
      resveratrol-treated and non-treated cells (B> 0, is the odd thatthe gene is 
      differentially expressed). Inhibitor of DNA binding 1 (ID1), histidine-rich 
      glycoprotein (HRG), NADPH oxidase (NOX1) and sprouty homolog 1 (SPRY), were 
      upregulated by LPS treatment, but significantly blocked by trans-resveratrol 
      pre-treatment (padj< 0.05, after adjusting for Benjamini-Hocheberg procedure). 
      Moreover, genes implicated in synthesis of lipids (z-score= -1.195) and 
      concentration of cholesterol (z-score= -0.109), were markedly downregulated by 
      trans-resveratrol. Other genes involved in fat turnover, but also in cell death 
      and survival function, such as transcription factors Kruppel-like factor 5 (KLF5) 
      and amphiregulin (AREG), were also significantly inhibited by trans-resveratrol 
      pre-treatment. RT-qPCR-data confirmed the microarray results. Special mention 
      deserves acyl-CoA synthetase long-chain family member 3 (ACSL3) and endothelial 
      lipase (LIPG), which were downregulated by this stilbene and have been previously 
      associated with fatty acid synthesis and obesity in other tissues. This study 
      envisages that trans-resveratrol might exert an important anti-lipogenic effect 
      at intestinal level under pro-inflammatory conditions, which has not been 
      previously described.
FAU - Etxeberria, U
AU  - Etxeberria U
AD  - University of Navarra Department of Nutrition, Food Science and Physiology, 
      Centre for Nutrition Research Pamplona Spain.
FAU - Castilla-Madrigal, R
AU  - Castilla-Madrigal R
AD  - University of Navarra Department of Nutrition, Food Science and Physiology, 
      Centre for Nutrition Research Pamplona Spain.
FAU - Lostao, M P
AU  - Lostao MP
AD  - University of Navarra Department of Nutrition, Food Science and Physiology, 
      Centre for Nutrition Research Pamplona Spain.
FAU - Martinez, J A
AU  - Martinez JA
AD  - University of Navarra Department of Nutrition, Food Science and Physiology, 
      Centre for Nutrition Research Pamplona Spain jalfmtz@unav.es.
FAU - Milagro, F I
AU  - Milagro FI
AD  - University of Navarra Department of Nutrition, Food Science and Physiology, 
      Centre for Nutrition Research Pamplona Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151209
PL  - France
TA  - Cell Mol Biol (Noisy-le-grand)
JT  - Cellular and molecular biology (Noisy-le-Grand, France)
JID - 9216789
RN  - 0 (AREG protein, human)
RN  - 0 (Amphiregulin)
RN  - 0 (Anticholesteremic Agents)
RN  - 0 (EGF Family of Proteins)
RN  - 0 (ID1 protein, human)
RN  - 0 (Inhibitor of Differentiation Protein 1)
RN  - 0 (KLF5 protein, human)
RN  - 0 (Kruppel-Like Transcription Factors)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Membrane Proteins)
RN  - 0 (Phosphoproteins)
RN  - 0 (Proteins)
RN  - 0 (SPRY1 protein, human)
RN  - 0 (Stilbenes)
RN  - 0 (histidine-rich proteins)
RN  - 97C5T2UQ7J (Cholesterol)
RN  - EC 1.6.3.- (NADPH Oxidase 1)
RN  - EC 1.6.3.- (NADPH Oxidases)
RN  - EC 1.6.3.- (NOX1 protein, human)
RN  - EC 3.1.1.3 (LIPG protein, human)
RN  - EC 3.1.1.3 (Lipase)
RN  - EC 6.2.1.- (Coenzyme A Ligases)
RN  - EC 6.2.1.3 (long-chain-fatty-acid-CoA ligase)
RN  - Q369O8926L (Resveratrol)
SB  - IM
MH  - Amphiregulin
MH  - Anticholesteremic Agents/*pharmacology
MH  - Caco-2 Cells
MH  - Cell Death/drug effects/genetics
MH  - Cholesterol/*metabolism
MH  - Coenzyme A Ligases/genetics/metabolism
MH  - EGF Family of Proteins/genetics/metabolism
MH  - Enterocytes/cytology/*drug effects/metabolism
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Humans
MH  - Inhibitor of Differentiation Protein 1/genetics/metabolism
MH  - Kruppel-Like Transcription Factors/genetics/metabolism
MH  - Lipase/genetics/metabolism
MH  - Lipogenesis/*drug effects/genetics
MH  - Lipopolysaccharides/antagonists & inhibitors/pharmacology
MH  - Membrane Proteins/genetics/metabolism
MH  - Microarray Analysis
MH  - NADPH Oxidase 1
MH  - NADPH Oxidases/genetics/metabolism
MH  - Phosphoproteins/genetics/metabolism
MH  - Proteins
MH  - Resveratrol
MH  - Signal Transduction
MH  - Stilbenes/*pharmacology
EDAT- 2015/12/17 06:00
MHDA- 2016/09/15 06:00
CRDT- 2015/12/16 06:00
PHST- 2015/10/28 00:00 [received]
PHST- 2015/12/05 00:00 [accepted]
PHST- 2015/12/16 06:00 [entrez]
PHST- 2015/12/17 06:00 [pubmed]
PHST- 2016/09/15 06:00 [medline]
PST - epublish
SO  - Cell Mol Biol (Noisy-le-grand). 2015 Dec 9;61(8):9-16.