PMID- 26667930
OWN - NLM
STAT- MEDLINE
DCOM- 20170327
LR  - 20181113
IS  - 1432-0886 (Electronic)
IS  - 0009-5915 (Linking)
VI  - 125
IP  - 4
DP  - 2016 Sep
TI  - The 14/15 association as a paradigmatic example of tracing karyotype evolution in 
      New World monkeys.
PG  - 747-56
LID - 10.1007/s00412-015-0565-2 [doi]
AB  - Fluorescence in situ hybridization (FISH), especially chromosome painting, has 
      been extensively exploited in the phylogenetic reconstruction of primate 
      evolution. Although chromosome painting is a key method to map translocations, it 
      is not effective in detecting chromosome inversions, which may be up to four 
      times more frequent than other chromosomal rearrangements. BAC-FISH instead can 
      economically delineate marker order and reveal intrachromosomal rearrangements. 
      However, up to now, BAC-FISH was rarely used to study the chromosomes of New 
      World monkeys partly due to technical difficulties. In this paper, we used 
      BAC-FISH to disentangle the complex evolutionary history of the ancestral 14/15 
      association in NWMs, beginning from the squirrel monkey (Saimiri boliviensis). To 
      improve the hybridization efficiency of BAC-FISH in NWMs, we "translated" the 
      human BACs into Callithrix jacchus (CJA) BACs, which yielded much higher 
      hybridization efficiencies on other NWM species than human BACs. Our results 
      disclosed 14 synteny blocks in squirrel monkeys, 7 more than with chromosome 
      painting. We then applied a subset of CJA BACs on six other NWM species. The 
      comparison of the hybridization pattern of these species contained phylogenetic 
      information to discriminate evolutionary relationships. Notably Aotus was found 
      to share an inversion with Callithrix, thus definitely assigning the genus Aotus 
      to Cebidae. The present study can be seen as a paradigmatic approach to 
      investigate the phylogenetics of NWMs by molecular cytogenetics.
FAU - Capozzi, Oronzo
AU  - Capozzi O
AD  - Department of Biology, University of Bari, Bari, Italy.
FAU - Archidiacono, Nicoletta
AU  - Archidiacono N
AD  - Department of Biology, University of Bari, Bari, Italy.
FAU - Lorusso, Nicola
AU  - Lorusso N
AD  - Department of Biology, University of Bari, Bari, Italy.
FAU - Stanyon, Roscoe
AU  - Stanyon R
AD  - Department of Biology, University of Florence, Florence, Italy. 
      roscoe.stanyon@unifi.it.
FAU - Rocchi, Mariano
AU  - Rocchi M
AD  - Department of Biology, University of Bari, Bari, Italy. rocchi@biologia.uniba.it.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151214
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
SB  - IM
MH  - Animals
MH  - Atelinae
MH  - Biological Evolution
MH  - Cell Line
MH  - Chromosome Inversion/*genetics
MH  - Chromosome Painting/*methods
MH  - Chromosomes, Artificial, Bacterial/*genetics
MH  - Evolution, Molecular
MH  - Humans
MH  - Karyotype
MH  - Phylogeny
MH  - Pitheciidae
MH  - Synteny/*genetics
MH  - Translocation, Genetic/*genetics
OTO - NOTNLM
OT  - BAC-FISH
OT  - BLAT
OT  - Disrupted synteny
OT  - Molecular cytogenetics
OT  - Phylogenomics
OT  - Rapid chromosome evolution
EDAT- 2015/12/17 06:00
MHDA- 2017/03/28 06:00
CRDT- 2015/12/16 06:00
PHST- 2015/09/04 00:00 [received]
PHST- 2015/11/30 00:00 [accepted]
PHST- 2015/11/24 00:00 [revised]
PHST- 2015/12/16 06:00 [entrez]
PHST- 2015/12/17 06:00 [pubmed]
PHST- 2017/03/28 06:00 [medline]
AID - 10.1007/s00412-015-0565-2 [pii]
AID - 10.1007/s00412-015-0565-2 [doi]
PST - ppublish
SO  - Chromosoma. 2016 Sep;125(4):747-56. doi: 10.1007/s00412-015-0565-2. Epub 2015 Dec 
      14.