PMID- 26667931
OWN - NLM
STAT- MEDLINE
DCOM- 20170327
LR  - 20240426
IS  - 1432-0886 (Electronic)
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 125
IP  - 4
DP  - 2016 Sep
TI  - Mapping centromeres of microchromosomes in the zebra finch (Taeniopygia guttata) 
      using half-tetrad analysis.
PG  - 757-68
LID - 10.1007/s00412-015-0560-7 [doi]
AB  - Centromeres usually consist of hundreds of kilobases of repetitive sequence which 
      renders them difficult to assemble. As a consequence, centromeres are often 
      missing from assembled genomes and their locations on physical chromosome maps 
      have to be inferred from flanking sequences via fluorescence in situ 
      hybridization (FISH). Alternatively, centromere positions can be mapped using 
      linkage analyses in accidentally triploid individuals formed by half-tetrads 
      (resulting from the inheritance of two chromatids from a single meiosis). The 
      current genome assembly of the zebra finch (Taeniopygia guttata) comprises 32 
      chromosomes, but only for the ten largest chromosomes centromere positions have 
      been mapped using FISH. We here map the positions of most of the remaining 
      centromeres using half-tetrad analyses. For this purpose, we genotyped 37 zebra 
      finches that were triploid or tetraploid due to inheritance errors (and mostly 
      died as embryos) together with their parents at 64 microsatellite markers (at 
      least two per chromosome). Using the information on centromere positions on the 
      ten largest chromosomes, we were able to identify 12 cases of non-disjunction in 
      maternal meiosis I and 10 cases of non-disjunction in maternal meiosis II. These 
      22 informative cases allowed us to infer centromere positions on additional 19 
      microchromosomes in reference to the current genome assembly. This knowledge will 
      be valuable for studies of chromosome evolution, meiotic drive and species 
      divergence in the avian lineage.
FAU - Knief, Ulrich
AU  - Knief U
AD  - Department of Behavioural Ecology and Evolutionary Genetics, Max Planck Institute 
      for Ornithology, 82319, Seewiesen, Germany. uknief@orn.mpg.de.
FAU - Forstmeier, Wolfgang
AU  - Forstmeier W
AD  - Department of Behavioural Ecology and Evolutionary Genetics, Max Planck Institute 
      for Ornithology, 82319, Seewiesen, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151215
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
SB  - IM
MH  - Animals
MH  - Centromere/*physiology
MH  - Chromosomes/genetics/*ultrastructure
MH  - Finches/*genetics
MH  - Genetic Linkage
MH  - Genome/genetics
MH  - Microsatellite Repeats/genetics
MH  - Physical Chromosome Mapping/methods/*veterinary
MH  - Spindle Apparatus/*metabolism
MH  - Telomere/*physiology
PMC - PMC5023761
OTO - NOTNLM
OT  - Chromosomal anomalies
OT  - Half-tetrad
OT  - Linkage analysis
OT  - Polyploidy
OT  - Telomere
COIS- Compliance with ethical standards Ethical standards All institutional and 
      national guidelines for the care and use of laboratory animals were followed. 
      Conflict of interest The authors declare that they have no competing interests.
EDAT- 2015/12/17 06:00
MHDA- 2017/03/28 06:00
PMCR- 2015/12/15
CRDT- 2015/12/16 06:00
PHST- 2015/09/14 00:00 [received]
PHST- 2015/11/13 00:00 [accepted]
PHST- 2015/11/12 00:00 [revised]
PHST- 2015/12/16 06:00 [entrez]
PHST- 2015/12/17 06:00 [pubmed]
PHST- 2017/03/28 06:00 [medline]
PHST- 2015/12/15 00:00 [pmc-release]
AID - 10.1007/s00412-015-0560-7 [pii]
AID - 560 [pii]
AID - 10.1007/s00412-015-0560-7 [doi]
PST - ppublish
SO  - Chromosoma. 2016 Sep;125(4):757-68. doi: 10.1007/s00412-015-0560-7. Epub 2015 Dec 
      15.