PMID- 26670356
OWN - NLM
STAT- MEDLINE
DCOM- 20170119
LR  - 20170119
IS  - 1896-494X (Electronic)
IS  - 1232-1087 (Linking)
VI  - 29
IP  - 2
DP  - 2016
TI  - Occupational exposure to 50 Hz magnetic fields does not alter responses of 
      inflammatory genes and activation of splenic lymphocytes in mice.
PG  - 277-91
LID - 58554 [pii]
LID - 10.13075/ijomeh.1896.00519 [doi]
AB  - OBJECTIVES: The objective of the present study was to observe the effects of 50 
      Hz magnetic fields (MFs) on the immune function of splenic lymphocytes in mice. 
      MATERIAL AND METHODS: Twenty male Kunming mice (6 weeks old), weighing 18- 25 g, 
      were randomly divided into sham exposure (N = 10) and 500 muT MFs (N = 10) groups. 
      The mice in the MFs group were exposed to 500 muT MFs for 8 h daily (5 days/week) 
      for up to 60 days. In vitro study was carried out to examine the effects of 50 Hz 
      MFs on the expression of inflammatory factor genes and a cluster of 
      differentiation 69 (CD69) in mouse prime splenic lymphocytes activated by 
      para-Methoxyamphetamine (PMA) and ionomycin. In the in vitro experiments, 
      lymphocytes were isolated from the spleen of 10 healthy Kunming mice, the cells 
      were cultured in the Roswell Park Memorial Institute 1640 medium (RPMI-1640) and 
      exposed to 0 muT, 250 muT, 500 muT, or 1 mT MFs in an incubator under 5% carbon 
      dioxide (CO2) at 37 degrees C for 6 h. The levels of interleukin-2 (IL-2), IL-4, 
      interferon-gamma (IFN-gamma), GATA binding protein 3 (GATA-3) and T cell-specific 
      T-box transcription factor (T-bet) were assessed by the real-time quantitative 
      reverse transcription polymerase chain reaction (RT-qPCR), respectively. The 
      expression of CD69 was checked using the flow cytometry. RESULTS: Under our 
      experimental conditions, body weight of the mice exposed to occupational, 
      extremely low frequency- electromagnetic fields (ELF-EMFs) significantly 
      decreased on day 20 and day 30. There were no significant changes observed in 
      vivo in spleen weight, splenic coefficient, splenic histology profile and 
      cytokine production in spleen tissues. Our in vitro experiments showed that 50 Hz 
      MFs had no effect on the expression of these genes and CD69 to primary splenic 
      cells. CONCLUSIONS: In conclusion, under the applied experimental conditions, 
      occupational exposure to 50 Hz magnetic field did not alter responses of 
      inflammatory genes and activation of splenic lymphocytes in mice, except for body 
      weight.
CI  - This work is available in Open Access model and licensed under a CC BY-NC 3.0 PL 
      license.
FAU - Luo, Xue
AU  - Luo X
AD  - Third Military Medical University, Chongqing, China (Institute of Tropical 
      Medicine). luoxuecq@hotmail.com.
FAU - Jia, Shujie
AU  - Jia S
AD  - Eighth Hospital of Xi'an, Shaanxi, China (Department of Pharmacy). 
      jiashujie8855@hotmail.com.
FAU - Li, Renyan
AU  - Li R
AD  - Key Laboratory of Birth Defects and Reproductive Health, Chongqing, China 
      (Chongqing Institute of Population and Family Planning). lirenyan2011@sina.cn.
FAU - Gao, Peng
AU  - Gao P
AD  - Third Military Medical University, Chongqing, China (Department of Occupational 
      Health).
FAU - Zhang, Yanwen
AU  - Zhang Y
AD  - Third Military Medical University, Chongqing, China (Department of Occupational 
      Health). zhangyw20022002@aliyun.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Poland
TA  - Int J Occup Med Environ Health
JT  - International journal of occupational medicine and environmental health
JID - 9437093
RN  - 0 (Cytokines)
SB  - IM
MH  - Animals
MH  - Cytokines/*metabolism
MH  - Disease Models, Animal
MH  - Inflammation/*genetics/metabolism
MH  - Lymphocytes/*pathology/radiation effects
MH  - Magnetic Fields
MH  - Male
MH  - Mice
MH  - Occupational Exposure/*adverse effects
MH  - Spleen/*pathology
OTO - NOTNLM
OT  - cytokine
OT  - inflammation
OT  - lymphocytes
OT  - magnetic fields
OT  - mice
OT  - spleen
EDAT- 2015/12/17 06:00
MHDA- 2017/01/20 06:00
CRDT- 2015/12/17 06:00
PHST- 2015/12/17 06:00 [entrez]
PHST- 2015/12/17 06:00 [pubmed]
PHST- 2017/01/20 06:00 [medline]
AID - 58554 [pii]
AID - 10.13075/ijomeh.1896.00519 [doi]
PST - ppublish
SO  - Int J Occup Med Environ Health. 2016;29(2):277-91. doi: 
      10.13075/ijomeh.1896.00519.