PMID- 26671198
OWN - NLM
STAT- MEDLINE
DCOM- 20160927
LR  - 20151216
IS  - 1091-7691 (Electronic)
IS  - 0895-8378 (Linking)
VI  - 27
IP  - 14
DP  - 2015
TI  - Cigarette smoke extract-treated mast cells promote alveolar macrophage 
      infiltration and polarization in experimental chronic obstructive pulmonary 
      disease.
PG  - 822-31
LID - 10.3109/08958378.2015.1116644 [doi]
AB  - OBJECTIVE: Cigarette smoking is the main cause of chronic obstructive pulmonary 
      disease (COPD) and may modulate the immune response of exposed individuals. Mast 
      cell function can be altered by cigarette smoking, but the role of smoking in 
      COPD remains poorly understood. The current study aimed to explore the role of 
      cigarette smoke extract (CSE)-treated mast cells in COPD pathogenesis. METHODS: 
      Cytokine and chemokine expression as well as degranulation of bone marrow-derived 
      mast cells (BMMCs) were detected in cells exposed to immunoglobulin E (IgE) and 
      various doses of CSE. Adoptive transfer of CSE-treated BMMCs into C57BL/6J mice 
      was performed, and macrophage infiltration and polarization were evaluated by 
      fluorescence-activated cell sorting (FACS). Furthermore, a coculture system of 
      BMMCs and macrophages was established to examine macrophage phenotype transition. 
      The role of protease serine member S31 (Prss31) was also investigated in the 
      co-culture system and in COPD mice. RESULTS: CSE exposure suppressed cytokine 
      expression and degranulation in BMMCs, but promoted the expressions of chemokines 
      and Prss31. Adoptive transfer of CSE-treated BMMCs induced macrophage 
      infiltration and M2 polarization in the mouse lung. Moreover, CSE-treated BMMCs 
      triggered macrophage M2 polarization via Prss31 secretion. Recombinant Prss31 was 
      shown to activate interleukin (IL)-13/IL-13Ralpha/Signal transducers and activators 
      of transcription (Stat) 6 signaling in macrophages. Additionally, a positive 
      correlation was found between Prss31 expression and the number of M2 macrophages 
      in COPD mice. CONCLUSION: In conclusion, CSE-treated mast cells may induce 
      macrophage infiltration and M2 polarization via Prss31 expression, and 
      potentially contribute to COPD progression.
FAU - Li, Hong
AU  - Li H
AD  - a Department of Respiratory and Critical Care Medicine , The First Affiliated 
      Hospital of Xi'an Jiaotong University , Xi'an , China.
FAU - Yang, Tian
AU  - Yang T
AD  - a Department of Respiratory and Critical Care Medicine , The First Affiliated 
      Hospital of Xi'an Jiaotong University , Xi'an , China.
FAU - Ning, Qian
AU  - Ning Q
AD  - a Department of Respiratory and Critical Care Medicine , The First Affiliated 
      Hospital of Xi'an Jiaotong University , Xi'an , China.
FAU - Li, Feiyan
AU  - Li F
AD  - a Department of Respiratory and Critical Care Medicine , The First Affiliated 
      Hospital of Xi'an Jiaotong University , Xi'an , China.
FAU - Chen, Tianjun
AU  - Chen T
AD  - a Department of Respiratory and Critical Care Medicine , The First Affiliated 
      Hospital of Xi'an Jiaotong University , Xi'an , China.
FAU - Yao, Yan
AU  - Yao Y
AD  - a Department of Respiratory and Critical Care Medicine , The First Affiliated 
      Hospital of Xi'an Jiaotong University , Xi'an , China.
FAU - Sun, Zhongmin
AU  - Sun Z
AD  - a Department of Respiratory and Critical Care Medicine , The First Affiliated 
      Hospital of Xi'an Jiaotong University , Xi'an , China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Inhal Toxicol
JT  - Inhalation toxicology
JID - 8910739
RN  - 0 (Complex Mixtures)
RN  - 0 (Cytokines)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Smoke)
RN  - EC 3.4.21.59 (Prss31 protein, mouse)
RN  - EC 3.4.21.59 (Tryptases)
SB  - IM
MH  - Animals
MH  - Complex Mixtures/*toxicity
MH  - Cytokines/genetics/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Lung/cytology/drug effects/pathology
MH  - Macrophages, Alveolar/*physiology
MH  - Male
MH  - Mast Cells/*drug effects
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Pulmonary Disease, Chronic Obstructive/*chemically induced/pathology
MH  - Recombinant Proteins/genetics/metabolism
MH  - Smoke/*adverse effects/analysis
MH  - Tobacco Products/*adverse effects/analysis
MH  - Tryptases/genetics/metabolism
OTO - NOTNLM
OT  - M2 polarization
OT  - Prss31
OT  - chronic objective pulmonary disease
OT  - cigarette smoke extract
OT  - macrophage
OT  - mast cell
EDAT- 2015/12/17 06:00
MHDA- 2016/09/28 06:00
CRDT- 2015/12/17 06:00
PHST- 2015/12/17 06:00 [entrez]
PHST- 2015/12/17 06:00 [pubmed]
PHST- 2016/09/28 06:00 [medline]
AID - 10.3109/08958378.2015.1116644 [doi]
PST - ppublish
SO  - Inhal Toxicol. 2015;27(14):822-31. doi: 10.3109/08958378.2015.1116644.