PMID- 26671478
OWN - NLM
STAT- MEDLINE
DCOM- 20160429
LR  - 20151216
IS  - 1559-4106 (Electronic)
IS  - 1559-4106 (Linking)
VI  - 10
IP  - 4
DP  - 2015 Dec 15
TI  - ToF-SIMS multivariate analysis of surface-grafted small bioactive molecules.
PG  - 04A310
LID - 10.1116/1.4937464 [doi]
AB  - In the development of bioactive coatings on biomaterials, it is essential to 
      characterize the successful fabrication and the uniformity of intended coatings 
      by sensitive surface analytical techniques, so as to ensure reliable 
      interpretation of observed biointerfacial responses. This can, however, be 
      challenging when small bioactive molecules are grafted onto biomaterials surfaces 
      at sub- and near-monolayer densities. Time-of-flight secondary ion mass 
      spectrometry (ToF-SIMS) provides the required sensitivity, but ion signals from 
      small grafted molecules may still be dominated by fragment ions from the 
      underlying polymer. In such cases, multivariate analysis provides valuable 
      enhancement of spectral data, as illustrated here by examples comprising the 
      surface grafting of bioactive serrulatane molecules, the peptide GRGDSP, the 
      oligonucleotide 15-thymidine, and the antifungal compound Amphotericin B. The 
      authors also show how ToF-SIMS plus principal component analysis can distinguish 
      between covalent grafting and physisorption of the antibiotics caspofungin and 
      micafungin.
FAU - Jasieniak, Marek
AU  - Jasieniak M
AD  - Mawson Institute, University of South Australia, Mawson Lakes, South Australia 
      5095, Australia.
FAU - Coad, Bryan R
AU  - Coad BR
AD  - Mawson Institute, University of South Australia, Mawson Lakes, South Australia 
      5095, Australia.
FAU - Griesser, Hans J
AU  - Griesser HJ
AD  - Mawson Institute, University of South Australia, Mawson Lakes, South Australia 
      5095, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151215
PL  - United States
TA  - Biointerphases
JT  - Biointerphases
JID - 101275679
RN  - 0 (Biological Products)
RN  - 0 (Coated Materials, Biocompatible)
SB  - IM
MH  - Biological Products/*analysis
MH  - Coated Materials, Biocompatible/*chemistry
MH  - Multivariate Analysis
MH  - Spectrometry, Mass, Secondary Ion
MH  - *Surface Properties
EDAT- 2015/12/17 06:00
MHDA- 2016/04/30 06:00
CRDT- 2015/12/17 06:00
PHST- 2015/12/17 06:00 [entrez]
PHST- 2015/12/17 06:00 [pubmed]
PHST- 2016/04/30 06:00 [medline]
AID - 10.1116/1.4937464 [doi]
PST - epublish
SO  - Biointerphases. 2015 Dec 15;10(4):04A310. doi: 10.1116/1.4937464.