PMID- 26672200
OWN - NLM
STAT- MEDLINE
DCOM- 20151229
LR  - 20190923
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 33
IP  - 7
DP  - 2015 Jul
TI  - [Analysis of 17 cytokinins in rice by solid phase extraction purification and 
      liquid chromatography-tandem mass spectrometry].
PG  - 715-21
AB  - A method was developed for the determination of 17 cytokinins in rice by solid 
      phase extraction and liquid chromatography-tandem mass spectrometry. The rice 
      samples were cryogenically ground under liquid nitrogen and extracted with 
      methanol-water (80:20, v/v) at 4  degrees C for 16 h. The supernatant was purified on a 
      column packed with polymer cation exchange resin (PCX). The samples were 
      transported and eluted on an analytical column ZORBAX Extend-C18 (100 mm x 2.1 
      mm, 1.8 mum) by methanol and 5 mmol/L ammonium formate aqueous solution. All the 
      analytes were detected in selected reaction monitoring (SRM) mode under positive 
      electrospray ionization (ESI+) and quantified by external standard method. The 
      separation conditions were optimized in order to achieve the sufficient 
      separation for the several isomers of cytokinins, such as 
      trans-zeatin-7-glucoside (tZ7G), trans-zeatin-9-glucoside (tZ9G), and 
      trans-zeatin-O-glucoside (tZOG). Moreover, the extraction efficiency of different 
      extraction solvents and clean-up effects of PCX cartridge for each analyte were 
      further investigated. The results showed that the correlation coefficients were 
      not less than 0.9984 in the linear range, and the limits of detection were ranged 
      from 0.01 to 0.05 ng/g. The mean recoveries of the 17 cytokinins at three spiked 
      levels of 0.2, 1 and 5 ng/g were from 60.2% to 125.4% with the relative standard 
      deviations (RSDs) of 5.4%-29.7% (n = 6). Finally, five endogenous cytokinins were 
      successfully quantified in real sample, and their contents were between 0.02 and 
      0.93 ng/g. It means that this method is reliable for quantitative analysis of 
      cytokinins in rice.
FAU - Cao, Zhaoyun
AU  - Cao Z
FAU - Ma, Youning
AU  - Ma Y
FAU - Mou, Renxiang
AU  - Mou R
FAU - Yu, Shasha
AU  - Yu S
FAU - Chen, Mingxue
AU  - Chen M
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 0 (Cytokinins)
SB  - IM
MH  - Chromatography, Liquid
MH  - Cytokinins/*chemistry
MH  - Mass Spectrometry
MH  - Oryza/*chemistry
MH  - Solid Phase Extraction
EDAT- 2015/12/18 06:00
MHDA- 2015/12/30 06:00
CRDT- 2015/12/18 06:00
PHST- 2015/12/18 06:00 [entrez]
PHST- 2015/12/18 06:00 [pubmed]
PHST- 2015/12/30 06:00 [medline]
AID - 10.3724/sp.j.1123.2015.03013 [doi]
PST - ppublish
SO  - Se Pu. 2015 Jul;33(7):715-21. doi: 10.3724/sp.j.1123.2015.03013.