PMID- 26674009
OWN - NLM
STAT- MEDLINE
DCOM- 20170106
LR  - 20170107
IS  - 1096-0805 (Electronic)
IS  - 0022-2011 (Linking)
VI  - 133
DP  - 2016 Jan
TI  - Detection and distribution of ostreid herpesvirus 1 in experimentally infected 
      Pacific oyster spat.
PG  - 59-65
LID - S0022-2011(15)30042-2 [pii]
LID - 10.1016/j.jip.2015.11.013 [doi]
AB  - High mortality rates are reported in spat and larvae of Pacific oyster 
      Crassostrea gigas and associated with ostreid herpesvirus 1 (OsHV-1) detection in 
      France. Although the viral infection has been experimentally reproduced in oyster 
      larvae and spat, little knowledge is currently available concerning the viral 
      entry and its distribution in organs and tissues. This study compares OsHV-1 DNA 
      and RNA detection and localization in experimentally infected oysters using two 
      virus doses: a low dose that did not induce any mortality and a high dose 
      inducing high mortality. Real time PCR demonstrated significant differences in 
      terms of viral DNA amounts between the two virus doses. RNA transcripts were 
      detected in oysters receiving the highest dose of viral suspension whereas no 
      transcript was observed in oysters injected with the low dose. This study also 
      allowed observing kinetics of viral DNA and RNA detection in different tissues of 
      oyster spat. Finally, viral detection was significantly different in function of 
      tissues (p<0.005), time (p<0.005) with an interaction between tissues and time 
      (p<0.005) for each probe.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Segarra, Amelie
AU  - Segarra A
AD  - Ifremer (Institut Francais de Recherche pour l'Exploitation de la Mer), Unite 
      Sante, Genetique et Microbiologie des Mollusques (SG2M), Laboratoire de Genetique 
      et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La 
      Tremblade, France.
FAU - Baillon, Laury
AU  - Baillon L
AD  - Ifremer (Institut Francais de Recherche pour l'Exploitation de la Mer), Unite 
      Sante, Genetique et Microbiologie des Mollusques (SG2M), Laboratoire de Genetique 
      et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La 
      Tremblade, France.
FAU - Faury, Nicole
AU  - Faury N
AD  - Ifremer (Institut Francais de Recherche pour l'Exploitation de la Mer), Unite 
      Sante, Genetique et Microbiologie des Mollusques (SG2M), Laboratoire de Genetique 
      et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La 
      Tremblade, France.
FAU - Tourbiez, Delphine
AU  - Tourbiez D
AD  - Ifremer (Institut Francais de Recherche pour l'Exploitation de la Mer), Unite 
      Sante, Genetique et Microbiologie des Mollusques (SG2M), Laboratoire de Genetique 
      et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La 
      Tremblade, France.
FAU - Renault, Tristan
AU  - Renault T
AD  - Ifremer (Institut Francais de Recherche pour l'Exploitation de la Mer), Unite 
      Sante, Genetique et Microbiologie des Mollusques (SG2M), Laboratoire de Genetique 
      et Pathologie des Mollusques Marins (LGPMM), Avenue de Mus de Loup, 17390 La 
      Tremblade, France. Electronic address: Tristan.Renault@ifremer.fr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151207
PL  - United States
TA  - J Invertebr Pathol
JT  - Journal of invertebrate pathology
JID - 0014067
RN  - 0 (DNA, Viral)
RN  - 0 (RNA, Viral)
SB  - IM
MH  - Animals
MH  - DNA, Viral/analysis/chemistry
MH  - Herpesviridae/isolation & purification/*physiology
MH  - Host-Pathogen Interactions
MH  - In Situ Hybridization
MH  - Kinetics
MH  - Larva/virology
MH  - Ostreidae/*virology
MH  - RNA, Viral/analysis/chemistry
MH  - Real-Time Polymerase Chain Reaction
MH  - Virus Internalization
OTO - NOTNLM
OT  - Crassostrea gigas
OT  - In situ hybridization
OT  - Ostreid herpesvirus 1
OT  - Viral DNA
OT  - Viral RNA
EDAT- 2015/12/18 06:00
MHDA- 2017/01/07 06:00
CRDT- 2015/12/18 06:00
PHST- 2015/08/31 00:00 [received]
PHST- 2015/11/27 00:00 [revised]
PHST- 2015/11/30 00:00 [accepted]
PHST- 2015/12/18 06:00 [entrez]
PHST- 2015/12/18 06:00 [pubmed]
PHST- 2017/01/07 06:00 [medline]
AID - S0022-2011(15)30042-2 [pii]
AID - 10.1016/j.jip.2015.11.013 [doi]
PST - ppublish
SO  - J Invertebr Pathol. 2016 Jan;133:59-65. doi: 10.1016/j.jip.2015.11.013. Epub 2015 
      Dec 7.