PMID- 26674381
OWN - NLM
STAT- MEDLINE
DCOM- 20161028
LR  - 20170731
IS  - 1673-8527 (Print)
IS  - 1673-8527 (Linking)
VI  - 42
IP  - 11
DP  - 2015 Nov 20
TI  - Competitive Expression of Endogenous Wheat CENH3 May Lead to Suppression of Alien 
      ZmCENH3 in Transgenic Wheat x Maize Hybrids.
PG  - 639-649
LID - S1673-8527(15)00136-8 [pii]
LID - 10.1016/j.jgg.2015.05.006 [doi]
AB  - Uniparental chromosome elimination in wheat x maize hybrid embryos is widely used 
      in double haploid production of wheat. Several explanations have been proposed 
      for this phenomenon, one of which is that the lack of cross-species CENH3 
      incorporation may act as a barrier to interspecies hybridization. However, it is 
      unknown if this mechanism applies universally. To study the role of CENH3 in 
      maize chromosome elimination of wheat x maize hybrid embryos, maize ZmCENH3 and 
      wheat alphaTaCENH3-B driven by the constitutive CaMV35S promoter were transformed 
      into wheat variety Yangmai 158. Five transgenic lines for ZmCENH3 and six 
      transgenic lines for alphaTaCENH3-B were identified. RT-PCR analysis showed that the 
      transgene could be transcribed at a low level in all ZmCENH3 transgenic lines, 
      whereas transcription of endogenous wheat CENH3 was significantly up-regulated. 
      Interestingly, the expression levels of both wheat CENH3 and ZmCENH3 in the 
      ZmCENH3 transgenic wheat x maize hybrid embryos were higher than those in the 
      non-transformed Yangmai 158 x maize hybrid embryos. This indicates that the alien 
      ZmCENH3 in wheat may induce competitive expression of endogenous wheat CENH3, 
      leading to suppression of ZmCENH3 over-expression. Eliminations of maize 
      chromosomes in hybrid embryos of ZmCENH3 transgenic wheat x maize and Yangmai 
      158 x maize were compared by observations on micronuclei presence, by marker 
      analysis using maize SSRs (simple sequence repeats), and by FISH (fluorescence in 
      situ hybridization) using 45S rDNA as a probe. The results indicate that maize 
      chromosome elimination events in the two crosses are not significantly different. 
      Fusion protein ZmCENH3-YFP could not be detected in ZmCENH3 transgenic wheat by 
      either Western blotting or immnunostaining, whereas accumulation and loading of 
      the alphaTaCENH3-B-GFP fusion protein was normal in alphaTaCENH3-B transgenic lines. As 
      ZmCENH3-YFP did not accumulate after AM114 treatment, we speculate that low 
      levels of ZmCENH3 protein in transgenic wheat may be one of the factors that lead 
      to failure of suppression of maize chromatin elimination in ZmCENH3 transgenic 
      wheat x maize hybrids.
CI  - Copyright (c) 2015 Institute of Genetics and Developmental Biology, Chinese Academy 
      of Sciences, and Genetics Society of China. Published by Elsevier Ltd. All rights 
      reserved.
FAU - Chen, Wei
AU  - Chen W
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics 
      Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China.
FAU - Zhu, Qilin
AU  - Zhu Q
AD  - National Maize Improvmental Center, China Agricultural University, Beijing 
      100193, China.
FAU - Wang, Haiyan
AU  - Wang H
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics 
      Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China.
FAU - Xiao, Jin
AU  - Xiao J
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics 
      Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China.
FAU - Xing, Liping
AU  - Xing L
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics 
      Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China.
FAU - Chen, Peidu
AU  - Chen P
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics 
      Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China.
FAU - Jin, Weiwei
AU  - Jin W
AD  - National Maize Improvmental Center, China Agricultural University, Beijing 
      100193, China. Electronic address: weiweijin@cau.edu.cn.
FAU - Wang, Xiu-E
AU  - Wang XE
AD  - State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics 
      Institute, Nanjing Agricultural University/JCIC-MCP, Nanjing 210095, China. 
      Electronic address: xiuew@njau.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20150810
PL  - China
TA  - J Genet Genomics
JT  - Journal of genetics and genomics = Yi chuan xue bao
JID - 101304616
RN  - 0 (Plant Proteins)
SB  - IM
MH  - Chromosomes, Plant/genetics/metabolism
MH  - Down-Regulation
MH  - *Gene Expression Regulation, Plant
MH  - *Hybridization, Genetic
MH  - Plant Proteins/*genetics/metabolism
MH  - Plants, Genetically Modified/*genetics/metabolism
MH  - Triticum/*genetics/metabolism
MH  - Zea mays/*genetics/metabolism
OTO - NOTNLM
OT  - CENH3
OT  - Transgenic wheat
OT  - Triticum aestivum
OT  - Uniparental chromosome elimination
OT  - Zea mays
EDAT- 2015/12/18 06:00
MHDA- 2016/11/01 06:00
CRDT- 2015/12/18 06:00
PHST- 2015/01/21 00:00 [received]
PHST- 2015/04/15 00:00 [revised]
PHST- 2015/05/06 00:00 [accepted]
PHST- 2015/12/18 06:00 [entrez]
PHST- 2015/12/18 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
AID - S1673-8527(15)00136-8 [pii]
AID - 10.1016/j.jgg.2015.05.006 [doi]
PST - ppublish
SO  - J Genet Genomics. 2015 Nov 20;42(11):639-649. doi: 10.1016/j.jgg.2015.05.006. 
      Epub 2015 Aug 10.