PMID- 26674416
OWN - NLM
STAT- MEDLINE
DCOM- 20160920
LR  - 20220310
IS  - 2046-2441 (Electronic)
IS  - 2046-2441 (Linking)
VI  - 5
IP  - 12
DP  - 2015 Dec
TI  - Transcriptional and phenotypical heterogeneity of Trypanosoma cruzi cell 
      populations.
PG  - 150190
LID - 10.1098/rsob.150190 [doi]
LID - 150190
AB  - Trypanosoma cruzi has a complex life cycle comprising pools of cell populations 
      which circulate among humans, vectors, sylvatic reservoirs and domestic animals. 
      Recent experimental evidence has demonstrated the importance of clonal variations 
      for parasite population dynamics, survival and evolution. By limiting dilution 
      assays, we have isolated seven isogenic clonal cell lines derived from the Pan4 
      strain of T. cruzi. Applying different molecular techniques, we have been able to 
      provide a comprehensive characterization of the expression heterogeneity in the 
      mucin-associated surface protein (MASP) gene family, where all the clonal 
      isogenic populations were transcriptionally different. Hierarchical cluster 
      analysis and sequence comparison among different MASP cDNA libraries showed that, 
      despite the great variability in MASP expression, some members of the 
      transcriptome (including MASP pseudogenes) are conserved, not only in the 
      life-cycle stages but also among different strains of T. cruzi. Finally, other 
      important aspects for the parasite, such as growth, spontaneous metacyclogenesis 
      or excretion of different catabolites, were also compared among the clones, 
      demonstrating that T. cruzi populations of cells are also phenotypically 
      heterogeneous. Although the evolutionary strategy that sustains the MASP 
      expression polymorphism remains unknown, we suggest that MASP clonal variability 
      and phenotypic heterogeneities found in this study might provide an advantage, 
      allowing a rapid response to environmental pressure or changes during the life 
      cycle of T. cruzi.
CI  - (c) 2015 The Authors.
FAU - Seco-Hidalgo, Victor
AU  - Seco-Hidalgo V
AD  - Biochemistry and Molecular Parasitology Research Group, Department of 
      Parasitology, University of Granada, Campus de Fuentenueva, Granada, Spain 
      vsecoh@gmail.com.
FAU - De Pablos, Luis Miguel
AU  - De Pablos LM
AD  - Biochemistry and Molecular Parasitology Research Group, Department of 
      Parasitology, University of Granada, Campus de Fuentenueva, Granada, Spain Centre 
      for Immunology and Infection (CII), Biology Department, University of York, York, 
      UK luis.depablostorro@york.ac.uk.
FAU - Osuna, Antonio
AU  - Osuna A
AD  - Biochemistry and Molecular Parasitology Research Group, Department of 
      Parasitology, University of Granada, Campus de Fuentenueva, Granada, Spain 
      aosuna@ugr.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Open Biol
JT  - Open biology
JID - 101580419
RN  - 0 (Membrane Proteins)
RN  - 0 (Protozoan Proteins)
SB  - IM
MH  - Membrane Proteins/genetics/*metabolism
MH  - Protozoan Proteins/genetics/*metabolism
MH  - Trypanosoma cruzi/genetics/*metabolism
PMC - PMC4703061
OTO - NOTNLM
OT  - RNA
OT  - gene expression
OT  - kinetoplastid
OT  - mucin-associated surface protein
OT  - protozoan
EDAT- 2015/12/18 06:00
MHDA- 2016/09/22 06:00
PMCR- 2015/12/16
CRDT- 2015/12/18 06:00
PHST- 2015/12/18 06:00 [entrez]
PHST- 2015/12/18 06:00 [pubmed]
PHST- 2016/09/22 06:00 [medline]
PHST- 2015/12/16 00:00 [pmc-release]
AID - rsob.150190 [pii]
AID - rsob150190 [pii]
AID - 10.1098/rsob.150190 [doi]
PST - ppublish
SO  - Open Biol. 2015 Dec;5(12):150190. doi: 10.1098/rsob.150190.