PMID- 26674618
OWN - NLM
STAT- MEDLINE
DCOM- 20160510
LR  - 20220309
IS  - 1545-7885 (Electronic)
IS  - 1544-9173 (Print)
IS  - 1544-9173 (Linking)
VI  - 13
IP  - 12
DP  - 2015 Dec
TI  - Prolonged Intracellular Na+ Dynamics Govern Electrical Activity in Accessory 
      Olfactory Bulb Mitral Cells.
PG  - e1002319
LID - 10.1371/journal.pbio.1002319 [doi]
LID - e1002319
AB  - Persistent activity has been reported in many brain areas and is hypothesized to 
      mediate working memory and emotional brain states and to rely upon network or 
      biophysical feedback. Here, we demonstrate a novel mechanism by which persistent 
      neuronal activity can be generated without feedback, relying instead on the slow 
      removal of Na+ from neurons following bursts of activity. We show that mitral 
      cells in the accessory olfactory bulb (AOB), which plays a major role in 
      mammalian social behavior, may respond to a brief sensory stimulation with 
      persistent firing. By combining electrical recordings, Ca2+ and Na+ imaging, and 
      realistic computational modeling, we explored the mechanisms underlying the 
      persistent activity in AOB mitral cells. We found that the exceptionally slow 
      inward current that underlies this activity is governed by prolonged dynamics of 
      intracellular Na+ ([Na+]i), which affects neuronal electrical activity via 
      several pathways. Specifically, elevated dendritic [Na+]i reverses the Na+-Ca2+ 
      exchanger activity, thus modifying the [Ca2+]i set-point. This process, which 
      relies on ubiquitous membrane mechanisms, is likely to play a role in other 
      neuronal types in various brain regions.
FAU - Zylbertal, Asaph
AU  - Zylbertal A
AD  - Department of Neurobiology, Institute of Life Sciences, Hebrew University and the 
      Edmond and Lily Safra Center for Brain Sciences, Jerusalem, Israel.
FAU - Kahan, Anat
AU  - Kahan A
AD  - School of Medicine, Department of Medical Neurobiology, Hebrew University, 
      Jerusalem, Israel.
FAU - Ben-Shaul, Yoram
AU  - Ben-Shaul Y
AD  - School of Medicine, Department of Medical Neurobiology, Hebrew University, 
      Jerusalem, Israel.
FAU - Yarom, Yosef
AU  - Yarom Y
AD  - Department of Neurobiology, Institute of Life Sciences, Hebrew University and the 
      Edmond and Lily Safra Center for Brain Sciences, Jerusalem, Israel.
FAU - Wagner, Shlomo
AU  - Wagner S
AD  - Sagol Department of Neurobiology, University of Haifa, Haifa, Israel.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151216
PL  - United States
TA  - PLoS Biol
JT  - PLoS biology
JID - 101183755
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Sodium-Calcium Exchanger)
SB  - IM
CIN - PLoS Biol. 2015 Dec;13(12):e1002320. PMID: 26674750
MH  - Animals
MH  - Computational Biology/methods
MH  - Dendrites/*metabolism
MH  - Electrophysiological Phenomena
MH  - Female
MH  - Ion Channel Gating
MH  - Kinetics
MH  - Male
MH  - Mice, Inbred BALB C
MH  - Mice, Inbred C57BL
MH  - Microscopy, Fluorescence
MH  - Microscopy, Video
MH  - *Models, Neurological
MH  - Nerve Tissue Proteins/*metabolism
MH  - *Neural Conduction
MH  - Neurons/cytology/*metabolism
MH  - Olfactory Bulb/cytology/*metabolism
MH  - Single-Cell Analysis
MH  - Sodium-Calcium Exchanger/*metabolism
MH  - Specific Pathogen-Free Organisms
PMC - PMC4684409
COIS- The authors have declared that no competing interests exist.
EDAT- 2015/12/18 06:00
MHDA- 2016/05/11 06:00
PMCR- 2015/12/16
CRDT- 2015/12/18 06:00
PHST- 2015/09/07 00:00 [received]
PHST- 2015/11/05 00:00 [accepted]
PHST- 2015/12/18 06:00 [entrez]
PHST- 2015/12/18 06:00 [pubmed]
PHST- 2016/05/11 06:00 [medline]
PHST- 2015/12/16 00:00 [pmc-release]
AID - PBIOLOGY-D-15-02588 [pii]
AID - 10.1371/journal.pbio.1002319 [doi]
PST - epublish
SO  - PLoS Biol. 2015 Dec 16;13(12):e1002319. doi: 10.1371/journal.pbio.1002319. 
      eCollection 2015 Dec.