PMID- 26675801
OWN - NLM
STAT- MEDLINE
DCOM- 20160301
LR  - 20181202
IS  - 0376-2491 (Print)
IS  - 0376-2491 (Linking)
VI  - 95
IP  - 21
DP  - 2015 Jun 2
TI  - [Preparation of vanilline cross-linked rhBMP-2/chitosan microspheres and its 
      effect on mesenchymal stem cells].
PG  - 1691-4
AB  - OBJECTIVE: To prepare rhBMP-2/chitosan microspheres (rhBMP-2 CMs) with vanilline 
      as a cross-linking reagent and study the biocompatibility and drug release 
      characteristic of microspheres in vitro. METHODS: Emulsion cross-linking method 
      was utilized to prepare rhBMP-2 CMs, Scanning electron microscope (SEM) was used 
      to observe the microstructure of microspheres.Leaching solution of microspheres 
      and blank culture medium were designated as experimental and control groups 
      respectively. Both groups were cultured with human mesenchymal stem cells (hMSCs) 
      to determine its cytotoxicity and its effect on the proliferation of hMSCs. 
      Dynamic immersion method was used to examine the in vitro release characteristic 
      of rhBMP-2. And the alkaline phosphatase (ALP) activity of hMSCs was determined 
      to reveal the bioactivity of released rhBMP-2. RESULTS: The rhBMP-2 CMs were 
      spherical under SEM.After treating with leaching solution for 24 and 48 h, there 
      was no inter-group statistical difference in optical density (OD) values at both 
      timepoints (24 h:0.72 +/- 0.07 vs 0.73 +/- 0.05, P > 0.05; 48 h:1.19 +/- 0.11 vs 1.27 +/- 
      0.06, P > 0.05). After culturing with leaching solution for 1, 3 and 7 days, the 
      number of cells increased with time for both groups. And the OD values were not 
      statistically different at each timepoint. Five milligram rhBMP-2 CMs soaked for 
      19 days with a gradual release of rhBMP-2. The concentration of rhBMP-2 was 216.1 
      +/- 20.0 ng/ml at Day 19. At Days 3 and 7, the ALP activities of hMSCs were (0.50 +/- 
      0.07) and (0.68 +/- 0.06) micromol pNPP.min(-)(1).mg(-)(1) protein respectively and both were 
      higher than that of blank culture medium group (0.14 +/- 0.01) (P < 0.05). 
      CONCLUSION: With an excellent biocompatibility, rhBMP-2 CMs may be an ideal 
      carrier for control-released rhBMP-2 and encapsulated rhBMP-2 remains bioactive.
FAU - Wu, Gui
AU  - Wu G
AD  - Department of Orthopedic Surgery, Peking Union Medical College Hospital, Chinese 
      Academy of Medical Sciences & Peking Union Medical College, Beijing 100730, 
      China.
FAU - Wang, Hai
AU  - Wang H
FAU - Qiu, Guixing
AU  - Qiu G
FAU - Yu, Xin
AU  - Yu X
FAU - Su, Xinlin
AU  - Su X
FAU - Ma, Pei
AU  - Ma P
FAU - Yin, Bo
AU  - Yin B
FAU - Wu, Zhihong
AU  - Wu Z
AD  - Email: wuzh3000@126.com.
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Za Zhi
JT  - Zhonghua yi xue za zhi
JID - 7511141
RN  - 0 (BMP2 protein, human)
RN  - 0 (Bone Morphogenetic Protein 2)
RN  - 0 (Recombinant Proteins)
RN  - 9012-76-4 (Chitosan)
SB  - IM
MH  - Bone Morphogenetic Protein 2
MH  - Chitosan
MH  - Humans
MH  - *Mesenchymal Stem Cells
MH  - *Microspheres
MH  - Recombinant Proteins
EDAT- 2015/12/18 06:00
MHDA- 2016/03/02 06:00
CRDT- 2015/12/18 06:00
PHST- 2015/12/18 06:00 [entrez]
PHST- 2015/12/18 06:00 [pubmed]
PHST- 2016/03/02 06:00 [medline]
PST - ppublish
SO  - Zhonghua Yi Xue Za Zhi. 2015 Jun 2;95(21):1691-4.