PMID- 26676856
OWN - NLM
STAT- MEDLINE
DCOM- 20161019
LR  - 20181202
IS  - 1573-4838 (Electronic)
IS  - 0957-4530 (Linking)
VI  - 27
IP  - 1
DP  - 2016 Jan
TI  - Anti-Toxoplasma activity and impact evaluation of lyophilization, hot molding 
      process, and gamma-irradiation techniques on CLH-PLGA intravitreal implants.
PG  - 10
LID - 10.1007/s10856-015-5621-1 [doi]
AB  - Intraocular delivery systems have been developed to treat many eye diseases, 
      especially those affecting the posterior segment of the eye. However, ocular 
      toxoplasmosis, the leading cause of infectious posterior uveitis in the world, 
      still lacks an effective treatment. Therefore, our group developed an 
      intravitreal polymeric implant to release clindamycin, a potent anti-Toxoplasma 
      antibiotic. In this work, we used different techniques such as differential 
      scanning calorimetry, thermogravimetry, X-ray diffraction, scanning electron 
      microscopy, and fourier-transform infrared spectroscopy to investigate 
      drug/polymer properties while manufacturing the delivery system. We showed that 
      the lyophilization, hot molding process, and sterilization by gamma irradiation 
      did not change drug/polymer physical-chemistry properties. The drug was found to 
      be homogeneously dispersed into the poly lactic-co-glycolic acid (PLGA) chains 
      and the profile release was characterized by an initial burst followed by 
      prolonged release. The drug profile release was not modified after gamma 
      irradiation and non-covalent interaction was found between the drug and the PLGA. 
      We also observed the preservation of the drug activity by showing the potent 
      anti-Toxoplasma effect of the implant, after 24-72 h in contact with cells 
      infected by the parasite, which highlights this system as an alternative to treat 
      toxoplasmic retinochoroiditis.
FAU - Fernandes-Cunha, Gabriella M
AU  - Fernandes-Cunha GM
AD  - Faculty of Pharmacy of the Federal University of Minas Gerais, Av. Presidente 
      Antonio Carlos, 6627, Belo Horizonte, MG, 31270-901, Brazil. 
      gabriellafcunha@gmail.com.
FAU - Rezende, Cintia M F
AU  - Rezende CM
AD  - Department of Biochemistry and Immunology Department, Institute of Biological 
      Science of the Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Mussel, Wagner N
AU  - Mussel WN
AD  - Chemistry Department of the Federal University of Minas Gerais, Belo Horizonte, 
      MG, Brazil.
FAU - da Silva, Gisele R
AU  - da Silva GR
AD  - School of Pharmacy, Federal University of Sao Joao del-Rei, Divinopolis, MG, 
      Bazil.
FAU - de L Gomes, Elionai C
AU  - de L Gomes EC
AD  - Laboratory of Thermal Analysis, Chemistry Department of the Federal University of 
      Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Yoshida, Maria I
AU  - Yoshida MI
AD  - Laboratory of Thermal Analysis, Chemistry Department of the Federal University of 
      Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Fialho, Silvia L
AU  - Fialho SL
AD  - Ezequiel Dias Foundation, Belo Horizonte, MG, Brazil.
FAU - Goes, Alfredo M
AU  - Goes AM
AD  - Department of Biochemistry and Immunology Department, Institute of Biological 
      Science of the Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Gomes, Dawison A
AU  - Gomes DA
AD  - Department of Biochemistry and Immunology Department, Institute of Biological 
      Science of the Federal University of Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - de Almeida Vitor, Ricardo W
AU  - de Almeida Vitor RW
AD  - Department of Parasitology, Institute of Biological Science of the Federal 
      University of Minas Gerais, Belo Horizonte, MG, Brazil.
FAU - Silva-Cunha, Armando
AU  - Silva-Cunha A
AD  - Faculty of Pharmacy of the Federal University of Minas Gerais, Av. Presidente 
      Antonio Carlos, 6627, Belo Horizonte, MG, 31270-901, Brazil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151216
PL  - United States
TA  - J Mater Sci Mater Med
JT  - Journal of materials science. Materials in medicine
JID - 9013087
RN  - 0 (Antiprotozoal Agents)
RN  - 1SIA8062RS (Polylactic Acid-Polyglycolic Acid Copolymer)
RN  - 26009-03-0 (Polyglycolic Acid)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 3U02EL437C (Clindamycin)
SB  - IM
MH  - Antiprotozoal Agents/*administration & dosage
MH  - Calorimetry, Differential Scanning
MH  - Cell Line
MH  - Clindamycin/*administration & dosage
MH  - Freeze Drying
MH  - *Gamma Rays
MH  - *Hot Temperature
MH  - Humans
MH  - *Lactic Acid
MH  - Microscopy, Electron, Scanning
MH  - *Polyglycolic Acid
MH  - Polylactic Acid-Polyglycolic Acid Copolymer
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Thermogravimetry
MH  - Toxoplasma/*drug effects
MH  - *Vitreous Body
MH  - X-Ray Diffraction
EDAT- 2015/12/18 06:00
MHDA- 2016/11/12 06:00
CRDT- 2015/12/18 06:00
PHST- 2015/05/04 00:00 [received]
PHST- 2015/11/05 00:00 [accepted]
PHST- 2015/12/18 06:00 [entrez]
PHST- 2015/12/18 06:00 [pubmed]
PHST- 2016/11/12 06:00 [medline]
AID - 10.1007/s10856-015-5621-1 [pii]
AID - 10.1007/s10856-015-5621-1 [doi]
PST - ppublish
SO  - J Mater Sci Mater Med. 2016 Jan;27(1):10. doi: 10.1007/s10856-015-5621-1. Epub 
      2015 Dec 16.