PMID- 26677200
OWN - NLM
STAT- MEDLINE
DCOM- 20161101
LR  - 20220410
IS  - 1556-679X (Electronic)
IS  - 1556-6811 (Print)
IS  - 1556-679X (Linking)
VI  - 23
IP  - 2
DP  - 2016 Feb
TI  - Serum Concentrations of Antibodies against Outer Membrane Protein P6, Protein D, 
      and T- and B-Cell Combined Antigenic Epitopes of Nontypeable Haemophilus 
      influenzae in Children and Adults of Different Ages.
PG  - 155-61
LID - 10.1128/CVI.00506-15 [doi]
AB  - Nontypeable Haemophilus influenzae (NTHi) is one of the most common etiologies of 
      acute otitis media, rhinosinusitis, and pneumonia. Outer membrane proteins (OMPs) 
      are the main focus in new vaccine development against NTHi, as the H. influenzae 
      type b (Hib) vaccine does not cover noncapsulated NTHi. The OMPs P6 and protein D 
      are the most promising candidate antigens for an NTHi vaccine, and low antibody 
      levels against them in serum may be correlated with infection caused by NTHi. In 
      the current study, we measured the antibody titers against P6, protein D, and 
      their T- and B-cell combined peptide epitopes in healthy individuals of different 
      ages. We found that children <1 month old had the lowest antibody levels against 
      NTHi P6, protein D, and their T- and B-cell combined antigenic epitopes. Antibody 
      titers increased at ages 1 to 6 months, peaked at 7 months to 3 years, and 
      remained high at 4 to 6 years. The antibody titers started to decrease after 6 
      years and were the lowest in the 21- to 30-year group. The geometric mean titers 
      (GMTs) of T- and B-cell combined antigenic epitopes in P6 and protein D were 
      positively correlated with those of the protein antigens. Among 12 peptides 
      tested, P6-61, P6-123, and protein D-167 epitopes were better recognized than 
      others in human serum. These findings might contribute to the development of an 
      effective serotype-independent vaccine for H. influenzae.
CI  - Copyright (c) 2016, American Society for Microbiology. All Rights Reserved.
FAU - Hua, Chun-Zhen
AU  - Hua CZ
AD  - Division of Infectious Diseases, Children's Hospital, Zhejiang University School 
      of Medicine, Hangzhou, Zhejiang, People's Republic of China.
FAU - Hu, Wei-Lin
AU  - Hu WL
AD  - Department of Medical Microbiology and Parasitology, Zhejiang University School 
      of Medicine, Hangzhou, Zhejiang, People's Republic of China Division of Basic 
      Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of 
      Infectious Diseases, First Affiliated Hospital, Zhejiang University School of 
      Medicine, Hangzhou, Zhejiang, People's Republic of China.
FAU - Shang, Shi-Qiang
AU  - Shang SQ
AD  - Department of Clinical Laboratory, Children's Hospital, Zhejiang University 
      School of Medicine, Hangzhou, Zhejiang, People's Republic of China 
      shangsq33@sina.com med_bp@zju.edu.cn.
FAU - Li, Jian-Ping
AU  - Li JP
AD  - Department of Clinical Laboratory, Children's Hospital, Zhejiang University 
      School of Medicine, Hangzhou, Zhejiang, People's Republic of China.
FAU - Hong, Li-Quan
AU  - Hong LQ
AD  - Department of Clinical Laboratory, The Affiliated Hospital of Hangzhou Normal 
      University, Hangzhou, Zhejiang, People's Republic of China.
FAU - Yan, Jie
AU  - Yan J
AD  - Department of Medical Microbiology and Parasitology, Zhejiang University School 
      of Medicine, Hangzhou, Zhejiang, People's Republic of China Division of Basic 
      Medical Microbiology, State Key Laboratory for Diagnosis and Treatment of 
      Infectious Diseases, First Affiliated Hospital, Zhejiang University School of 
      Medicine, Hangzhou, Zhejiang, People's Republic of China shangsq33@sina.com 
      med_bp@zju.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151216
PL  - United States
TA  - Clin Vaccine Immunol
JT  - Clinical and vaccine immunology : CVI
JID - 101252125
RN  - 0 (Antibodies, Bacterial)
RN  - 0 (Bacterial Outer Membrane Proteins)
RN  - 0 (Bacterial Proteins)
RN  - 0 (Epitopes)
RN  - 0 (Haemophilus Vaccines)
RN  - 0 (Immunoglobulin G)
RN  - 0 (P6 outer membrane protein, Haemophilus)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Age Factors
MH  - Aged
MH  - Aged, 80 and over
MH  - Antibodies, Bacterial/*blood
MH  - Bacterial Outer Membrane Proteins/*immunology
MH  - Bacterial Proteins/chemistry/*immunology
MH  - Child
MH  - Child, Preschool
MH  - Epitopes
MH  - Female
MH  - Haemophilus Infections/immunology/microbiology/*prevention & control
MH  - Haemophilus Vaccines/*immunology
MH  - Haemophilus influenzae/classification/*immunology
MH  - Healthy Volunteers
MH  - Humans
MH  - Immunoglobulin G/*blood
MH  - Infant
MH  - Infant, Newborn
MH  - Male
MH  - Middle Aged
MH  - Young Adult
PMC - PMC4744915
EDAT- 2015/12/18 06:00
MHDA- 2016/11/02 06:00
PMCR- 2016/08/05
CRDT- 2015/12/18 06:00
PHST- 2015/09/18 00:00 [received]
PHST- 2015/12/07 00:00 [accepted]
PHST- 2015/12/18 06:00 [entrez]
PHST- 2015/12/18 06:00 [pubmed]
PHST- 2016/11/02 06:00 [medline]
PHST- 2016/08/05 00:00 [pmc-release]
AID - CVI.00506-15 [pii]
AID - 00506-15 [pii]
AID - 10.1128/CVI.00506-15 [doi]
PST - epublish
SO  - Clin Vaccine Immunol. 2015 Dec 16;23(2):155-61. doi: 10.1128/CVI.00506-15. Print 
      2016 Feb.